6356499
Descripción
Test por Anna Ullrich, actualizado hace más de 1 año
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Creado por Anna Ullrich
hace casi 8 años
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Pregunta 1
Pregunta
What's right: Synthetic lethality ...
Respuesta
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Combinatorial effects between alleles or substances
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Effects in synthetic biology
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An effect specific to a double mutation (not seen in the singles)
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An effect specific to a mutation in combination with a SMI-small molecule inhibitor (not seen by either mutation or SMI alone)
Pregunta 2
Pregunta
What's right: Phage lambda lysogen ...
Respuesta
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C1 represses lambda's lysogenic genes, c2 activates int- and c1 transcription, c3 stabilizes c2
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C1 represses lambda's lysogenic genes, c2 represses int- and c1 transcription, c3 destabilizes c2
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C1 represses lambda's lytic genes, c2 activates int- and c1 transcription, c3 stabilizes c2
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C1 represses lambda's lytic genes, c2 represses int- and c1 transcription, c3 destabilizes c2
Pregunta 3
Pregunta
What's right: Bacterial transformation
Respuesta
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The mechanism of gram+ and gram- DNA uptake is not related
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Gram- bacteria possess an outer membrane requiring an additional pore for DNA uptake
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Gram+ bacteria possess an outer membrane requiring an additional pore for DNA uptake
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Gram+ and gram- bacteria have a pilus structure, a dsDNA receptor and an inner membrane pore in common
Pregunta 4
Pregunta
What's right: Intra- vs extragenic complementation
Respuesta
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Genetic complementation between mutations in different genes does not require wild type alleles
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Extragenic complementation is rare and requires mutations in different functional domains
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Intragenic complementation is rare and depends on the presence of domains that function independently
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Molecular complementation depends on addition of the wild gene
Pregunta 5
Pregunta
What's right: Dominant vs epistatic
Respuesta
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a is epistatic to locus b , if the double mutant (a,a/b,b) has the same phenotype as the single mutant (a,a)
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a is epistatic to b, means: Pht(a,b)=Pht(a,B) .. (although Pht(A,b)≠Pht(A,B))
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dominant : Pht(A,A)=Pht(a,A)
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dominant relates alleles of 1 gene, epistatic relates an allele to another gene
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A is dominant over a: Pht(a,A) = Pht(a,a)
Pregunta 6
Pregunta
What's right: gene vs allele
Respuesta
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a ncRNA is not encoded by a gene, because it is not translated
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a centromere is not a gene, because it is not transcribed
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a bacterial cistron is not a gene, because it contains several functional units
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a gene is a functional unit in the genome, activated by transcription while an allele is a sequence variant of a gene
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a bacterial operon is not a gene, because it contains several functional units
Pregunta 7
Pregunta
What's right: Falsifyability
Respuesta
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According to Popper’s critical rationalism (P.c.r), a Sc.Hyp can’t be claimed true, without evidence
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According to P.c.r. a single experiment, if well performed, can prove a hypothesis forever
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According to P.c.r. a single experiment, if well performed, can disprove a hypothesis forever
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According to P.c.r. a scientific hypothesis must make a prediction, which can be proven wrong
Pregunta 8
Pregunta
What's right: Suppressor mutation
Respuesta
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A suppressor mutation blocks the expression of another gene
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A suppressor mutation can’t occur in the same gene
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A suppressor mutation must occur in tRNA
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A suppressor blocks the appearance of a mutant phenotype
Pregunta 9
Pregunta
What's right: What is the phenotype?
Respuesta
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Many genes do not cause a phenotype
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The phenotype of a given mutation must be detectable under all conditions
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The phenotype of a mutation can change according to the situation
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The phenotype ist the observable impact of an allele
Pregunta 10
Pregunta
Connect the topic with the correct boxes/key words ...
BACTERIAL RECOMBINEERING
Respuesta
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attB/P/L/R
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targetted DSB
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RE enzyme free assembly of bacterial, genome-size DNA
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Efficient, RE enzyme free transfer of inserts between plasmids
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Inhibition of RecBCD
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RE enzyme free sub cloning from large inserts
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RE enzyme free correction of genetic defects in vivo
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Bacterial immune system
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Exo, Beta, Gam
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Edition through DSB-repair
Pregunta 11
Pregunta
Connect the topic with the correct boxes/key words ...
GATEWAY TECHNOLOGY
Respuesta
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attB/P/L/R
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targetted DSB
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RE enzyme free assembly of bacterial, genome-sized DNA
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Efficient, RE enzyme free transfer of inserts between plasmids
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RE enzyme free sub cloning from large inserts
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RE enzyme free correction of genetic defects in vivo
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Bacterial immune system
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Exo, Beta, Gam
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Editing through DSB-repair
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Cloning from donor to recipient
Pregunta 12
Pregunta
Connect the topic with the correct boxes/key words ...
CRISPR
Respuesta
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attB/P/L/R
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targetted DSB
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RE enzyme free assembly of bacterial, genome-size DNA
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Efficient, RE enzyme free transfer of inserts between plasmids
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Inhibition of RecBCD
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RE enzyme free subcloning from large inserts
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RE enzyme free correction of genetic defects in vivo
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Bacterial immune system
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Exo, Beta, Gam
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Editing through DSB-repair
Pregunta 13
Pregunta
Connect the topic with the correct boxes/key words ...
YEAST RECOMBINEERING
Respuesta
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attB/P/L/R
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targetted DSB
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RE enzyme free assembly of bacterial, genome-size DNA
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Efficient, RE enzyme free transfer of inserts between plasmids
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Inhibition of RecBCD
-
RE enzyme free subcloning from large inserts
-
RE enzyme free correction of genetic defects in vivo
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Bacterial immune system
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Exo, Beta, Gam
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Editing through DSB-repair
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