Enterobeacteriaceae

How are Enterobacteraceae classified according to a gram stain?

All Enterobacteriaceae ferment glucose

Not all members of the Enterobacteriaceae family are cytochrom-oxidase negative.

Enterobacteriaceae are obligatory anaerobes

Match the Classification with the antigen Somatic [blank_start]O[blank_end] Envelope [blank_start]K[blank_end] Flagellar [blank_start]H[blank_end]

Match the Antigen with its description. Endotoxin, heat stable part of the outer cell wall [blank_start]O[blank_end] Heat Labile capsular polysaccharide surrounding the cell wall, covers the O antigen [blank_start]K[blank_end] Heat labile protein found in the flagellum of mobile Enterobacteriaceae [blank_start]H[blank_end]

Which Media are usually used for the isolation of Enterobacteriaceae?

What reaction would one expect to see on a blood agar if the organism was positive for Hydrogen Sulfide?

Match the IMViC test with the importance of the result it generates E. coli is always positive: If the bacteria is positive in this test it means that the bacteria contains tryptophanase to convert tryptophan to ammonia and indole [blank_start]Indole[blank_end] E. Coli is always positive: If the bacteria is positive in this test it means it is able to convert glucose to pyruvic acid [blank_start]Methyl Red[blank_end] E. Coli is negative/Klebsiella is Positive: If the bacteria is positive in this test it means that it is able to convert glucose to acetoin [blank_start]Voges-Prokauer[blank_end] E. Coli is negative/Kelbsiella is positive. If the bacteria is positive in this test it means that it uses citrate as its main source of carbon [blank_start]Citrate[blank_end]

Select the sugars present in Triple Sugar Iron (TSI) agar

Which test is used to look for slow/late fermenters?

Which test is used to determine if an organism can convert urea into ammonia, carbon dioxide and water?

What colour will a Urease test change to if the organism is Urease positive?

Match the urea producer will its speed of production Proetus [blank_start]fast producer[blank_end] Klebsiella [blank_start]slow producer[blank_end]

Which two organisms will test negative in a motility test?

Which organisms will test positive for a Deaminase/Phenylalanine Deaminsae (PAD) test?

Match the test colour result with its corresponding conclusion. Acid Production [blank_start]Yellow[blank_end] No Acid Production [blank_start]Red[blank_end] Base production due to use of peptone by organism [blank_start]Pink[blank_end]

Which test is a good choice when one wants to distinguish Enterobacteriaceae from gram negative rods that do NOT ferment glucose?

Match the colour result from a Triple Sugar Iron (TSI) test with the correct conclusion. Bacteria ferments glucose but not sucrose or lactose. Alkaline slant over acid deep (K/A). [blank_start]Red over Yellow[blank_end] Bacteria ferments glucose, lactose and/or sucrose. Acid slant over Acid deep (A/A). [blank_start]Yellow over Yellow[blank_end] Bacteria is not able to ferment glucose. Alkaline Slant over Alkaline deep (K/K) [blank_start]Red over Red[blank_end] Production of gas [blank_start]Cracks or medium pulling away[blank_end] Bacteria produces Hydrogen Sulfide (H2s) [blank_start]Black precipitate[blank_end]

Which Species of Enterobacteriaceae will produce an acid slant and a deep acid (A/A) result with Gas production and no production of Hydrogen Sulfide in a Triple Sugar Iron (TSI) test.

Which species of Enterobacteriaceae will produce an Alkaline slant over a deep acid with positive gas production and positive hydrogen sulfide production in a Triple Sugar Iron (TSI) test?

Which species of Enterobacteriaceae will produce an Alkaline slant over a deep acid with negative gas production and negative hyrdogen sulfide production in a Triple Sugar Iron (TSI) test?

Which species of Enterobacteriaceae will produce an Alkaline slant over a deep alkalin with negative gas production and negative hyrdogen sulfide production in a Triple Sugar Iron (TSI) test?