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Microbiology Test 1 2nd Year PMU

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Microbiology Test 1 2nd Year PMU
Med Student
Quiz by Med Student , updated more than 1 year ago
Med Student
Created by Med Student over 7 years ago
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Question 1

Question
Name the components of nutrient media
Answer
  • Glucose Broth
  • Meat-Peptone Broth
  • Meat-Peptone agar
  • Blood Agar

Question 2

Question
Most of the bacterial colonies are visualized after:
Answer
  • 20 minutes
  • 2 hours
  • 1-2 days
  • 1 week

Question 3

Question
According to their cell wall structures bacteria divide into
Answer
  • Aerobes and Anarobes
  • Prokaryotes and Eukaryotes
  • Gram (+) and Gram (-)
  • Prototrophs and Auxotrophs

Question 4

Question
Extra chromosomal genetic elements in bacteria are
Answer
  • Mesosomes
  • Phagosomes
  • Transposons
  • Plasmids

Question 5

Question
How many phases have growth curve of bacteria? Ans: [blank_start]4[blank_end] Name them: a/ [blank_start]Lag[blank_end] Phase b/ [blank_start]Log[blank_end] Phase c/ [blank_start]Stationary[blank_end] Phase d/ [blank_start]Decline[blank_end] Phase
Answer
  • 4
  • Lag
  • Log
  • Stationary
  • Decline

Question 6

Question
Sex-pili of bacteria helps for their
Answer
  • Movement
  • Conjugation
  • Nutrition
  • Protection

Question 7

Question
Name the three shapes of bacteria: a/ [blank_start]Round[blank_end] ([blank_start]Cocci[blank_end]) b/ [blank_start]Rods[blank_end] c/ [blank_start]Spiral[blank_end] ([blank_start]Spirilli[blank_end])
Answer
  • Round
  • Cocci
  • Rods
  • Spiral
  • Spirilli

Question 8

Question
What is the most resistant to an unappropiate conditions bacterial form?
Answer
  • Vegetative
  • Spore

Question 9

Question
Which of the following bacterial structures prevents phagocytosis?
Answer
  • Cytoplasm
  • Peptidoglycan
  • Capsule
  • Nuclues

Question 10

Question
Are flagellas an obligatory structure of bacterium?
Answer
  • True
  • False

Question 11

Question
What chemical is responsible for the acid-fast property of mycobacteria? Ans: [blank_start]Mycolic acids[blank_end]
Answer
  • Mycolic acids

Question 12

Question
Name two bacteria that have capsules: a/ [blank_start]E. coli[blank_end] b/ [blank_start]Salmonella[blank_end]
Answer
  • E. coli
  • Salmonella

Question 13

Question
In microbiology what is the most commonly used objective Ans: [blank_start]Oil[blank_end] Explain: This is because [blank_start]oil[blank_end] unlike other liquids like water has very [blank_start]little light refraction[blank_end]
Answer
  • Oil
  • oil
  • little light refraction

Question 14

Question
What is the name of staining method for Corynebacterium diphtheriae?
Answer
  • Gram
  • Moller
  • Zhiehl-Neelsen
  • Neisser

Question 15

Question
Gram (+) bacteria are stained in [blank_start]blue[blank_end] colour and Gram (-) in [blank_start]red[blank_end] colour
Answer
  • blue
  • red

Question 16

Question
The main taxonomic category for bacteria is
Answer
  • Genus
  • Species
  • Family
  • Ordo

Question 17

Question
According Gram, the Gram (-) bacteria are stained in [blank_start]red[blank_end] colour
Answer
  • red

Question 18

Question
The unit for bacterial size is:
Answer
  • milimeter
  • micrometer
  • nanometer
  • angstrom

Question 19

Question
Vibrions are curved bacteria with
Answer
  • 4-6 curves
  • many curves
  • one curve
  • two curves

Question 20

Question
Ziehl-Neelsen is a staining procedure in:
Answer
  • encapsulated bacteria
  • spore-forming bacteria
  • acid-fast bacteria
  • motile bacteria

Question 21

Question
Bacterial cytoplasm contains:
Answer
  • capsule
  • ribosomes
  • nucleotide
  • peptidoglycan
  • pili
  • inclusions

Question 22

Question
Name the purposes of simple staining: a/ Determine cell [blank_start]shape[blank_end] b/ Determine cell [blank_start]size[blank_end] c/ Determine cell [blank_start]arrangement[blank_end]
Answer
  • shape
  • size
  • arrangement

Question 23

Question
The main staining procedure that detect morphology of bacteria is:
Answer
  • Loeffler
  • Neisser
  • Gram
  • Pfeiffer

Question 24

Question
The name Baccilus antracis defines certain bacterial
Answer
  • Genus
  • Species
  • Class

Question 25

Question
Is it true: Bacterial spores are formed in unfavorable conditions and are means of multiplying
Answer
  • True
  • False

Question 26

Question
Staining according Victoria blau is a [blank_start]simple[blank_end] method
Answer
  • simple
  • complex

Question 27

Question
Which of the following organelles are essential for bacteria?
Answer
  • cytoplasm membrane
  • pili
  • capsule
  • flagella
  • nucleoid
  • ribosomes

Question 28

Question
What are the curved bacteria?
Answer
  • staphylococci
  • helicobacter
  • vibrions
  • bacilli

Question 29

Question
Diplococci are [blank_start]round[blank_end] shaped bacteria that are arranged in [blank_start]pairs[blank_end]
Answer
  • round
  • pairs

Question 30

Question
Staphylococci are round shaped bacteria arranged in [blank_start]clusters[blank_end]
Answer
  • clusters

Question 31

Question
The volutine bodies are inclusions in the cytoplasm of:
Answer
  • menigococci
  • diptheria bacteria
  • genococci
  • pneumococci

Question 32

Question
Where is lipopolysaccaride situated?
Answer
  • in the cell wall of Gram (+) bacteria
  • in the cell wall of Gram (-) bacteria
  • in the both groups

Question 33

Question
Name at least two rod-shaped bacteria a/ [blank_start]Bacillus anthracis[blank_end] b/ [blank_start]Clostridium tetani[blank_end]
Answer
  • Bacillus anthracis
  • Clostridium tetani

Question 34

Question
How many spores form one bacteria?
Answer
  • one
  • two
  • three
  • more than one

Question 35

Question
Loefler technique stain bacteria in [blank_start]blue[blank_end] colour
Answer
  • blue

Question 36

Question
The causative agent of tuberculosis is stained in red color, using [blank_start]Ziehl[blank_end] [blank_start]Neelsen[blank_end] (name the staining method)
Answer
  • Ziehl
  • Neelsen

Question 37

Question
What are the two purposes of heat fixation a/ [blank_start]kill[blank_end] organisms b/ allows stain to be [blank_start]absorbed[blank_end]
Answer
  • kill
  • absorbed

Question 38

Question
Each bacteria has two names and they define its
Answer
  • family and genus
  • family and kingdom
  • genus and species

Question 39

Question
Bacteria cell is
Answer
  • eukaryotic
  • prokaryotic

Question 40

Question
Which of the following is NOT found in the cell wall of gram (-) bacteria?
Answer
  • Lipid A
  • Teichoic acids
  • O-antigen
  • peptidoglycan

Question 41

Question
Name the different types of bacteria pili and what are their function a/ [blank_start]common[blank_end] and its function is [blank_start]adhesion[blank_end] b/ [blank_start]sex[blank_end] and its function is [blank_start]conjugation[blank_end]
Answer
  • common
  • adhesion
  • sex
  • conjugation

Question 42

Question
Loeffler stain requires
Answer
  • Methylene blue
  • Ziehl fuschin
  • Gentian violet
  • Iodine solution

Question 43

Question
Which of the following methods of staining are complex
Answer
  • Pfeiffer
  • Neisser
  • Klett
  • Loeffler

Question 44

Question
Bacterium Diphteri and its structure : [blank_start]Volutine granule[blank_end]
Answer
  • Volutine granule

Question 45

Question
The volutine granules of Bacterium Diphteri are visualized by : [blank_start]Neisser[blank_end] staining
Answer
  • Neisser

Question 46

Question
The following structures are localized in the cytoplasm:
Answer
  • Spores
  • Nucleoids
  • Flagellum
  • Capsules

Question 47

Question
Based on their cellular structure bacteria are divided into:
Answer
  • Cocci and rods
  • Gram + and Gram –
  • Spares and spiral
  • Aerobes and anaerobe

Question 48

Question
Peptides Glycan polymer are found only in :
Answer
  • Bacterial cell
  • Animal cell
  • In viruses

Question 49

Question
Chemical structure of the capsule is composed of :
Answer
  • Complex polysaccharides
  • Vitamins
  • Polypeptides

Question 50

Question
Sporulation appears to be :
Answer
  • An initiation of reproduction of bacterial cell
  • A reaction of conditions that are unfavorable for normal growth
  • A status to resist desiccation and other harmful conditions
  • a & b
  • b & c

Question 51

Question
The complete killing or removal of all microorganisms including bacteria spores is called :
Answer
  • Sterilization
  • Disinfection
  • Antisepsis
  • Sepsis

Question 52

Question
What is the color of the simple method of Pfeiffer staining : [blank_start]red[blank_end]
Answer
  • red

Question 53

Question
Pfeiffer technical staining makes use of the following :
Answer
  • Methylene blue
  • Carbon Fuchsine
  • Neutral red
  • Gentian violet

Question 54

Question
The cell wall of gram (+) bacteria is composed mainly :
Answer
  • Hitine (typical for plants)
  • Peptidoglycan (90%)
  • Cellulose
  • Sugars

Question 55

Question
Which of the following bacteria have peptidoglycan layer:
Answer
  • Gram (+) cocci
  • Gram (-) bacteria
  • Both of them
  • None above

Question 56

Question
Cellular DNA is concentrated in (what place) :
Answer
  • Cytoplasm as a nucleoid
  • Mitochondria
  • Plasmids as an extra chromosomal genetic material
  • Ribosomes

Question 57

Question
The spores stain by Moller are :
Answer
  • Blue color
  • Red color
  • Black color
  • Yellow color

Question 58

Question
Which structure is NOT protecting bacteria from phagocytosis from leukocytes:
Answer
  • Spores
  • Capsule
  • Flagella
  • Ribosome

Question 59

Question
Name two simple (basic) stains a/ [blank_start]Loeffler[blank_end] b/ [blank_start]Pfeiffer[blank_end]
Answer
  • Loeffler
  • Pfeiffer

Question 60

Question
The concentration of ethyl alcohol as an antiseptic for disinfection is :
Answer
  • 95%
  • 70%
  • 50%
  • 30%

Question 61

Question
Bacterial nucleoid consists of :
Answer
  • No chromosomes
  • Only one chromosome
  • Several chromosomes

Question 62

Question
What is the name of staining procedure for bacterial capsule:
Answer
  • Gram stain
  • Loeffler stain
  • Klett stain
  • Moller stain

Question 63

Question
Lipopolysaccharide can be found in large amount :
Answer
  • Gram (+)
  • Gram (-)
  • Both of them
  • None of above

Question 64

Question
Complex method for bacterial staining are :
Answer
  • Loeffler
  • Pfeiffer
  • Gram
  • Ziehl-Neelsen

Question 65

Question
Bacterial antibiotic resistance is frequently covered by :
Answer
  • Tempered bacteriophage
  • R-plasmids
  • Lipid bacteriophage
  • Intron

Question 66

Question
What constitutes a bacterial culture : a/ [blank_start]Solute[blank_end] b/ dry [blank_start]agar[blank_end] c/ [blank_start]broth[blank_end]
Answer
  • Solute
  • agar
  • broth

Question 67

Question
Which one is a liquid medium for bacterial culture:
Answer
  • Blood agar
  • Glucose broth
  • Both of them
  • None above

Question 68

Question
Anaerobes are bacteria that can tolerate high oxygen concentration
Answer
  • True
  • False

Question 69

Question
What bacterial physiologist studies :
Answer
  • Morphology of bacteria
  • Bacterial structure
  • Bacterial growth
  • Bacterial nutrient

Question 70

Question
70% ethanol is :
Answer
  • Sterilizing agent
  • Antiseptic
  • Antibiotic
  • None is true

Question 71

Question
Which of the following is most important structure related to microbial attachment to cells :
Answer
  • Flagellum
  • Plasmid
  • Peptidoglycan
  • Ordinary Pilli

Question 72

Question
Which statement about genetic transfer among bacteria requires cell to cell contact:
Answer
  • Process of conjugation
  • Process of transduction
  • Process of transcription
  • Process of auto combination
  • Process of transformation

Question 73

Question
Which of the following related to endotoxin is not true:
Answer
  • Endotoxins are secreted from living cells
  • Endotoxins are part of cellular gram (-) bacteria
  • They are secreted only when the cell is disrupted
  • They can be linked to meningococcemia
  • They are produced by gram(-) microorganisms
  • They cause fever

Question 74

Question
What cause (hint) to be pink :
Answer
  • Carbon Fuchsin
  • Iodine solution
  • Crystal violet
  • Neisser A

Question 75

Question
Gram (-) bacteria differ from Gram (+) ones because the posses :
Answer
  • Lipopolysaccharide
  • Peptidoglycan
  • Only cytoplasmic membrane
  • Lipoteichoic acid

Question 76

Question
The auto-sterilisation requires the following :
Answer
  • 1:30 hours at 160 C
  • 134 C for 10-15 minutes

Question 77

Question
The chlorine is :
Answer
  • Antiseptic
  • Disinfectant
  • Chemical agent for sterilization
  • All of above

Question 78

Question
Concerning bacterial cell division everything is true except that :
Answer
  • All bacteria double their numbers every 10 hours
  • Bacterial generation times are influenced by environmental conditions
  • Two bacterial cells following division are genetically identical and they are once

Question 79

Question
How does high temperature cause cell death?
Answer
  • Oxidative damage
  • High level of electrolytes
  • Protein denature
  • All of above

Question 80

Question
How are the actions of phenols and alcohols similar :
Answer
  • Both denature proteins
  • Both oxidize DNA
  • Both inhibit nucleic acid synthesis
  • All of above

Question 81

Question
What is pasteurization :
Answer
  • A method that disinfects the instruments
  • Sterilizes food
  • A method that can be used on skin and mucus
  • All of above

Question 82

Question
Bacterial flagella are :
Answer
  • Composed of pillin
  • A floppy structure made of flagellin
  • Rigger structure made of flagellin

Question 83

Question
Name the complex nutrient media :
Answer
  • Glucose broth
  • Mid peptone broth
  • Mid peptone agar
  • Blood agar

Question 84

Question
Extra chromosomal genetic elements in bacteria are :
Answer
  • Mesosomes
  • Phagosomes
  • Transposomes
  • Bacteriophages ( or plasmids)

Question 85

Question
What part of the bacterial cell is most involved with Gram staining and why? Ans: [blank_start]Cell wall[blank_end] Explanation: Gram [blank_start]positive[blank_end] bacteria have a [blank_start]thick[blank_end] peptidoglycan cell [blank_start]wall[blank_end] that holds the stain [blank_start]better[blank_end] than the [blank_start]thin[blank_end] peptidoglycan gram [blank_start]negative[blank_end] cell [blank_start]wall[blank_end]
Answer
  • positive
  • thick
  • wall
  • better
  • thin
  • negative
  • wall
  • Cell wall

Question 86

Question
What is the purpose of heat during the acid-fast staining procedure? The heat [blank_start]drives[blank_end] the stain [blank_start]into[blank_end] the cells
Answer
  • drives
  • into

Question 87

Question
Name two curved bacteria: a. [blank_start]Vibrio Cholerae[blank_end] b. [blank_start]Helicobacteria[blank_end]
Answer
  • Vibrio Cholerae
  • Helicobacteria

Question 88

Question
Name the reagent used and state the purpose of each of the following a/ mordant: Reagent- [blank_start]Iodine[blank_end] solution, Function: form a [blank_start]complex[blank_end] between the crystal violet and [blank_start]iodine[blank_end] b/ primary stain: Reagent- [blank_start]Gentian Violet[blank_end], Function: increases the [blank_start]contrast[blank_end] of both gram-negative and gram-positive bacteria c/ decolorizer: Reagent- [blank_start]Alcohol[blank_end], Function: [blank_start]dehydrates[blank_end] the peptidoglycan layer d/ counterstain: Reagent- [blank_start]carbol fuschin[blank_end], Function: stains solution light [blank_start]red[blank_end] allowing Gram [blank_start]negative[blank_end] to be seen
Answer
  • Alcohol
  • Iodine
  • complex
  • iodine
  • Gentian Violet
  • contrast
  • dehydrates
  • carbol fuschin
  • red
  • negative

Question 89

Question
Round bacteria in chains are named [blank_start]streptococci[blank_end]
Answer
  • streptococci

Question 90

Question
What suggestions about endotoxins are true? a/ they are typical for [blank_start]Gram (-)[blank_end] b/ they act [blank_start]non-specific[blank_end] c/ their chemical structure is [blank_start]lipopolysaccarides[blank_end] d/ they are heat [blank_start]stable[blank_end]
Answer
  • Gram (-)
  • Gram (+)
  • non-specific
  • specific
  • lipopolysaccarides
  • proteins
  • stable
  • labile
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