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Microbiology Practical F

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Raymond MCC
Elle Ashe
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Elle Ashe
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Pregunta 1

Pregunta
Obligate intracellular parasite
Respuesta
  • Organisms that can only grow inside another living host.
  • Viruses that infect bacteria.
  • Viral replication cycle that ends in the death of the host cell.

Pregunta 2

Pregunta
Bacteriophage
Respuesta
  • Viruses that infect bacteria.
  • Organisms that can only grow inside another living host cell.
  • Viral replication cycle that ends in death of its host cell.

Pregunta 3

Pregunta
Lytic cycle
Respuesta
  • Viral replication cycle that ends in death of its host cell.
  • Organisms that can only grow inside another living host cell.
  • Viruses that infect bacteria.

Pregunta 4

Pregunta
How does a medium used for bacterial growth differ from media used for bacteriophage growth?
Respuesta
  • Use ordinary media to grow bacteria but, a bacteriophage needs a host cell.
  • Use ordinary media to grow bacteriophages but, bacteria needs a host cell.

Pregunta 5

Pregunta
What is the purpose of making serial dilutions of the T-4 phage?
Respuesta
  • To obtain a countable plate of 30 - 300 plaques.
  • To obtain a countable plate of 30 - 300 colonies.
  • To obtain a countable plate of 30 - 300 individual viruses.

Pregunta 6

Pregunta
What is a viral plaque and what does it represent?
Respuesta
  • An area of clearing on an agar representing a spot where a virus landed, infected a bacteria and lysed it.
  • An area on an agar representing a spot where a bacteria landed, infected a virus and lysed it.
  • An area on an agar representing a spot where a virus landed, infect a yeast and lysed it.

Pregunta 7

Pregunta
What purpose does the E. coli serve in this procedure?
Respuesta
  • It is the host cell for the T4 bacteriophage
  • It is the host cell of the T4 bacteria.
  • It is the host cell of the T4 fungus.

Pregunta 8

Pregunta
Explain why Coccidioides immitis is considered to be a dimorphic fungus.
Respuesta
  • It can grow as a yeast and as a mold.
  • It can grow as a yeast and a virus.
  • It can grow as a mold and a virus.

Pregunta 9

Pregunta
What are the growth differences between fungus and bacteria?
Respuesta
  • Fungi grow faster, at a lower temperature and at a lower pH than bacteria.
  • Fungi grow faster, at a higher temperature and at a higher pH than bacteria.
  • Fungi grow slower, at a lower temperature and at a lower pH than bacteria.
  • Fungi grow slower, at a higher temperature and at a higher pH than bacteria.

Pregunta 10

Pregunta
Staphylococcus and Micrococcus are"
Respuesta
  • Gram (+) cocci in clusters
  • Gram (-) cocci in clusters
  • Staphylococcus is gram (+) in clusters and Micrococcus is gram (-) in pairs
  • Staphylococcus is gram (-) in clusters and Micrococcus is gram (+) in pairs

Pregunta 11

Pregunta
Where are Staphylococcus and Micrococcus normally found in the body?
Respuesta
  • Skin and mucus membranes
  • Intestines
  • Gastrointestinal tract
  • Skin and hair

Pregunta 12

Pregunta
Where are Staphylococcus and Micrococcus normally found in the body?
Respuesta
  • Skin and mucus membranes
  • Intestines
  • Gastrointestinal tract
  • Skin and hair

Pregunta 13

Pregunta
People that harbor the pathogen but exhibit no symptoms.
Respuesta
  • Carrier
  • MRSA
  • Nosocomial
  • Giver

Pregunta 14

Pregunta
Methicillin-resistant Staphylococcus aureus
Respuesta
  • MRSA
  • Carrier
  • Nosocomial
  • Multiple Resistant Streptococcus Aureus

Pregunta 15

Pregunta
Hospital acquired
Respuesta
  • Nosocomial
  • Carrier
  • Giver
  • Catalase

Pregunta 16

Pregunta
Which substances that Staphylococcus aureus produce to cause disease
Respuesta
  • Catalase
  • Coagulase
  • Hyaluronidase
  • Carilase

Pregunta 17

Pregunta
When do staphylococcus epidermidis and Micrococcus cause disease?
Respuesta
  • They are opportunistic pathogens.
  • All the time
  • Never

Pregunta 18

Pregunta
Differentiate the colony appearance of Staphylococcus and Streptococcus
Respuesta
  • Staph are larger, convex, opague and pigmented, Strep are smaller, translucent to slightly opaque.
  • Strep are larger, convex, opague and pigmented, Straph are smaller, translucent to slightly opaque.
  • Staph are smaller, convex, opague and pigmented, Strep are larger, translucent to slightly opaque.

Pregunta 19

Pregunta
Which enzyme helps differentiate all staphylococci from streptococci?
Respuesta
  • Catalase
  • Streptocase
  • Staphoolase

Pregunta 20

Pregunta
How did Lancefield divide the beta-hemolytic streptococci into groups?
Respuesta
  • C polysaccharide extracted from the cell walls
  • Peptidoglycan extracted from the cell walls
  • Lipids extracted from the cell walls

Pregunta 21

Pregunta
What is the species name of Lancefield Group A streptococci.
Respuesta
  • Streptococcus pyogenes
  • Streptococcus aureus
  • Streptococcus epidermidis
  • Streptococcus agalaticae

Pregunta 22

Pregunta
What is the species name of Lancefield Group B streptococci?
Respuesta
  • Streptococcus agalaticae
  • Streptococcus aureus
  • Streptococcus pyogenes
  • Streptococcus cocci

Pregunta 23

Pregunta
Where are Enterococcus normally found int he body?
Respuesta
  • Gastrointestinal tract
  • Skin and mucus
  • Liver
  • Kidneys

Pregunta 24

Pregunta
VRE?
Respuesta
  • Vancomycin resistant enterococcus
  • Vancomycin resistant ebolacoccus
  • Vancomycin resistant enteroboccus

Pregunta 25

Pregunta
Viridans streptococci
Respuesta
  • Alpha hemolytic streptococci other than streptococcus pneumonea
  • Beta hemolytic streptococci other than streptococcus pneumonea
  • Alpha heolytic staphylococci other than staphylococcus pneumonea
  • Beta heolytic staphylococci other than staphylococcus pneumonea

Pregunta 26

Pregunta
Group A Strep causes __________________
Respuesta
  • Strep throat
  • Community-acquired pneumonia
  • Neonatal septicemia
  • Urinary tract infection

Pregunta 27

Pregunta
Group B Strep causes _____________
Respuesta
  • Neonatal septicemia
  • Strep throat
  • Urinary tract infection
  • Community-acquired pneumonia

Pregunta 28

Pregunta
Enterococcus causes _________________
Respuesta
  • Urinary tract infection
  • Dental cavities
  • Strep throat
  • Neonatal speticemia

Pregunta 29

Pregunta
Streptococcus pneumoniae causes _________________
Respuesta
  • Community-acquired pneumonia
  • Neonatal septicemia
  • Urinary tract infection
  • Strep throat

Pregunta 30

Pregunta
Viridans streptococci
Respuesta
  • Dental cavities
  • Strep throat
  • Urinary tract infection
  • Neonatal septicemia

Pregunta 31

Pregunta
Why is Coccidioides immitis considered to be a dimorphic fungus?
Respuesta
  • It can grow as a yeast and as a mold
  • It can grow in pairs.
  • It can grow as coccus or bacillus.

Pregunta 32

Pregunta
What are the growth factors between fungus and bacteria?
Respuesta
  • Fungi grow slower, at a lower temp and lower pH than bacteria
  • Fungi grow faster, at a higher temp and higher pH than bacteria
  • Fungi grow slower, at a higher temp and lower pH than bacteria
  • Fungi grow faster, at a lower temp and higher pH than bacteria

Pregunta 33

Pregunta
Explain the difference between vegetative hyphae and aerial hyphae.
Respuesta
  • Vegetative hyphae grow on or down into the agar surface; aerial hyphae grow above the agar surface.
  • Aerial hyphae grow on or down into the agar surface; vegetative hyphae grow above the agar surface.

Pregunta 34

Pregunta
The difference between sporangiospores and conidiospores:
Respuesta
  • Sporangipospores are inside of a sac and condidiospores are the end of hyphae.
  • Condidiospores are inside of a sac and sporangipospores are the end of hyphae.

Pregunta 35

Pregunta
How are molds identified in the laboratory?
Respuesta
  • Macroscopic appearance
  • Microscopic appearance
  • Hyphal structure

Pregunta 36

Pregunta
What are the characteristics of Enterobacteriacea?
Respuesta
  • All are gram (-) rods
  • All are gram (+) cocci
  • All ferment glucose
  • All reduce nitrate to nitrite
  • They are oxidase (-)
  • They are oxidase (+)
  • They do not ferment glucose

Pregunta 37

Pregunta
What are the characteristics of non-fermenters?
Respuesta
  • All are gram (-)
  • All ferment glucose
  • They do not ferment glucose
  • They are all oxidase (-)
  • Many are oxidase (+)
  • All are gram (+)

Pregunta 38

Pregunta
What are the two major groups of gram (-) rods?
Respuesta
  • Enterobacteriacaea
  • Non-fermenters
  • Staphylococcus
  • Streptococcus

Pregunta 39

Pregunta
Which carbohydrate is used to determine whether a gram negative rod is a "Nonfermenter" or "Enterobacteriacae"?
Respuesta
  • Glucose
  • Lactose

Pregunta 40

Pregunta
What carbohydrate(s) are in the Kigler Iron Agar media?
Respuesta
  • Glucose
  • Lactose
  • Sucrose
  • Galactose

Pregunta 41

Pregunta
What carbohydrate(s) are in the MacConkey agar?
Respuesta
  • Glucose
  • Lactose
  • Sucrose
  • Galactose

Pregunta 42

Pregunta
Why is MacConkey agar considered to be a selective and differential media?
Respuesta
  • It is selective because it contains bile salts and crystal violet which inhibit the growth of gram (+) organisms. It is differential because it contains lactose making it differential for lactose fermentation.
  • It is selective because it contains bile salts and crystal violet which inhibit the growth of gram (-) organisms. It is differential because it contains lactose making it differential for lactose fermentation.
  • It is differential because it contains bile salts and crystal violet which inhibit the growth of gram (+) organisms. It is selective because it contains lactose making it differential for lactose fermentation.
  • It is differential because it contains bile salts and crystal violet which inhibit the growth of gram (-) organisms. It is selective because it contains lactose making it differential for lactose fermentation.

Pregunta 43

Pregunta
How many types of organisms will you identify in the mixed unknown exercise?
Respuesta
  • 1
  • 2
  • 4

Pregunta 44

Pregunta
What type of organism grows on MacConkey agar?
Respuesta
  • Gram (-)
  • Gram (+)

Pregunta 45

Pregunta
What ingredient on the MacConkey agar will differentiate the organisms growing on the agar?
Respuesta
  • Lactose
  • Bile salts and crystal violet
  • Blood

Pregunta 46

Pregunta
What type of organism grows on CNA?
Respuesta
  • Gram (+)
  • Gram (-)

Pregunta 47

Pregunta
What ingredient in the CNA media allows differentiation of the organisms growing on the agar?
Respuesta
  • Bile salts and crystal violet
  • Blood
  • Lactose

Pregunta 48

Pregunta
Why is CNA media incubated in a candle jar?
Respuesta
  • Streptococcus are microaerophiles
  • Streptococcus are anaerobes

Pregunta 49

Pregunta
Why is a cotton swab used to obtain the sample from the original broth?
Respuesta
  • To make sure we get both organisms from the mixed culture
  • To make sure enough sample is collected
  • To make it easier to inoculate the media

Pregunta 50

Pregunta
Why is T. soy agar used to grow the "stock cultures"?
Respuesta
  • It's a neutral media that all bacteria like
  • It is a selective media that only gram (-) bacteria like.
  • It is a selective media that only gram (+) bacteria like.

Pregunta 51

Pregunta
Why do you need to develop two flow charts in order to identify your unknown organisms?
Respuesta
  • You have to develop a dichotomous key for each of your unknown organisms and we have two organisms.
  • You have to develop a dichotomous key for each depending on the outcome of growth on the selective media.

Pregunta 52

Pregunta
What is an "enteric" organism?
Respuesta
  • Part of the normal flora of the intestinal tract
  • Bacteria that live inside another organism.

Pregunta 53

Pregunta
How are culture media designed to detect if a bacteria can produce a specific enzyme?
Respuesta
  • Substrates are incorporated into the culture media that the enzymes act upon and that along with indicator system lets you see the products.
  • Enzymes are incorporated into the media and an indicator systems lets you see if they match the organism.

Pregunta 54

Pregunta
What is the purpose of the Durham tube in the carbohydrate fermentation tubes?
Respuesta
  • Collects gas
  • Releases gas
  • Keeps tube from exploding

Pregunta 55

Pregunta
Name the specific tests that are part of the IMViC test
Respuesta
  • Indole
  • Methyl red
  • Voges-Proskauer
  • Citrate
  • Urease

Pregunta 56

Pregunta
Name 3 Enterobacteriaceae normally found in the gastrointestinal tract.
Respuesta
  • E. coli
  • Enterobacter aerogenes
  • Proteus vulgaris
  • Salmonella
  • Klebsiella pneumoniae
  • Shigella

Pregunta 57

Pregunta
Name two Enterobacteriaceae that are major pathogens and state the disease they cause.
Respuesta
  • Salmonella - Gastroenteritis, typhoid fever
  • Shigella - Bacterial dysentery
  • E. coli - Gastroenteritis, typhoid fever
  • Proteus vulgaris - Bacterial dysentery

Pregunta 58

Pregunta
Which commercial test systems are used to identify gram (-) rods in clinical labs?
Respuesta
  • Entero tube
  • API
  • 20E
  • Micro ID
  • Micro tube
  • APE

Pregunta 59

Pregunta
Why do you use the streak-stab method for blood agar inoculations?
Respuesta
  • So you can see the hemolysis better
  • To see if the bacteria like to grow in or on the agar

Pregunta 60

Pregunta
Where do you grow streptococcus and why?
Respuesta
  • In a candle jar because it's a microaerophile
  • In the air because it's an aerobe

Pregunta 61

Pregunta
Which bacteria are alpha and optochin sensitive?
Respuesta
  • Streptococcus pneumoniae
  • Streptococcus mitis
  • Streptococcus pyogenes
  • Group C Streptococcus
  • Enterococcis faecalis

Pregunta 62

Pregunta
Which bacteria are and optochin resistant?
Respuesta
  • Streptococcus mitis
  • Streptococcus pneumoniae
  • Streptococcus pyogenes
  • Group C Streptococcus
  • Enterococcus faecalis

Pregunta 63

Pregunta
Which bacteria are beta and bacitracin sensitive?
Respuesta
  • Streptococcus pyogenes
  • Streptococcus pneumoniae
  • Streptococcus mitis
  • Group C Streptococcus
  • Enterococcus faecalis

Pregunta 64

Pregunta
Which bacteria are beta and bacitracin resistant?
Respuesta
  • Group C Streptococcus
  • Streptococcus mitis
  • Streptococcus pneumoniae
  • Enterococcus faecalis
  • Streptococcus pyogenes

Pregunta 65

Pregunta
How do you tell the difference between the alpha hemolytic Streptococcus and and Enterococcus faecalis?
Respuesta
  • The alpha Streptococcus are bile esculin (-) and the Enterococcus faecalis is bile esculin (+)
  • The alpha Streptococcus are bile esculin (+) and the Enterococcus faecalis is bile esculin (-)

Pregunta 66

Pregunta
How does the catalase test differentiate between Staphylococcus and Streptococcus?
Respuesta
  • Staphylococcus is catalase (+) and Streptococcus is catalase (-)
  • Staphylococcus is catalase (-) and Streptococcus is catalase (+)

Pregunta 67

Pregunta
What kind of hemolysis is shown on this plate?
Image:
3_Alpha_hemolysis_Strep_mitis_CNA (image/jpg)
Respuesta
  • Alpha hemolyisis
  • Beta Hemolysis
  • Gamma Hemolysis

Pregunta 68

Pregunta
What is the mold pictured?
Image:
3a_Aspergillus_Septate_hyphae (image/jpg)
Respuesta
  • Aspergillis
  • Penicillium
  • Rhizopus

Pregunta 69

Pregunta
What is the mold pictured?
Image:
3a_Aspergillus__10x__Lactophenol_cotton_blue (image/jpg)
Respuesta
  • Aspergillus
  • Penicillium
  • Rhizopus

Pregunta 70

Pregunta
What is the mold pictured?
Image:
3a_Aspergillus__10x__Lactophenol_cotton_blue (image/jpg)
Respuesta
  • Aspergillus
  • Penicillium
  • Rhizopus

Pregunta 71

Pregunta
What is the mold pictured?
Image:
3a_Aspergillus__400x (image/jpg)
Respuesta
  • Aspergillus
  • Penicillium
  • Rhuzopus

Pregunta 72

Pregunta
What is the mold pictured?
Image:
3a_Aspergillus__400x (image/jpg)
Respuesta
  • Aspergillus
  • Penicillium
  • Rhizopus

Pregunta 73

Pregunta
What is the mold pictured?
Image:
3a_Aspergillus_niger (image/jpg)
Respuesta
  • Aspergillus
  • Penicillium
  • Rhizopus

Pregunta 74

Pregunta
What is the mold pictured?
Image:
3a_Penicillium_Culture (image/jpg)
Respuesta
  • Aspergillus
  • Penicillium
  • Rhizopus

Pregunta 75

Pregunta
What mold is pictured?
Image:
3a_Fungi_Apergillus_slide_culture (image/jpg)
Respuesta
  • Aspergillus
  • Penicillium
  • Rhizopus

Pregunta 76

Pregunta
What mold is pictured?
Image:
3a_Micro_Fungi_Micro_Fungi_032 (image/jpg)
Respuesta
  • Aspergillus
  • Penicillium
  • Rhizopus

Pregunta 77

Pregunta
What mold is pictured?
Image:
3a_Penicillium_Slide_Culture (image/jpg)
Respuesta
  • Aspergillus
  • Penicillium
  • Rhizopus

Pregunta 78

Pregunta
What mold is pictured?
Image:
3a_Penicillium__Lactophenol_cotton_blue__40x (image/jpg)
Respuesta
  • Aspergillus
  • Penicillium
  • Rhizopus

Pregunta 79

Pregunta
What mold is pictured?
Image:
3a_Rhizopu_on_a_plate (image/jpg)
Respuesta
  • Aspergillus
  • Penicillium
  • Rhizopus

Pregunta 80

Pregunta
What mold is pictured?
Image:
3a_Rhizopus_zygospore__40x (image/jpg)
Respuesta
  • Aspergillus
  • Penicillium
  • Rhizopus

Pregunta 81

Pregunta
What mold is pictured?
Image:
3a_Rhizopus__40x (image/jpg)
Respuesta
  • Aspergillus
  • Penicillium
  • Rhizopus

Pregunta 82

Pregunta
What mold is pictured?
Image:
3a_rhizopus (image/jpg)
Respuesta
  • Aspergillus
  • Penicillium
  • Rhizopus

Pregunta 83

Pregunta
Is this result positive or negative for beta lactamase?
Image:
3aBeta_lactamase_test_positive_A (image/jpg)
Respuesta
  • Positive
  • Negative

Pregunta 84

Pregunta
Is this result positive or negative for beta lactamase?
Image:
3a_Beta_lactamase_test_negative_A (image/jpg)
Respuesta
  • Positive
  • Negative

Pregunta 85

Pregunta
What are the results of the bile esculin test pictured?
Image:
3_Bile (image/jpg)
Respuesta
  • A = negative, B = positive
  • A = positive, B = negative

Pregunta 86

Pregunta
What are the results of the carbohydrate fermentation pictured?
Image:
3_Carbohydrate_fermentation.JPG (image/JPG)
Respuesta
  • A = negative, B = positive, C = positive + gas
  • A = positive, B = negative, C = negative +gas

Pregunta 87

Pregunta
What are the results of the citrate test pictured?
Image:
3_Citrate.JPG (image/JPG)
Respuesta
  • A = negative, B = positive
  • A = positive, B = negative

Pregunta 88

Pregunta
What are the results of the indole test pictured?
Image:
3_INDOLE.JPG (image/JPG)
Respuesta
  • A = negative, B = positive
  • A = positive, B = negative

Pregunta 89

Pregunta
What is the result of the lactose fermentation pictured?
Image:
3_macconkey (image/jpg)
Respuesta
  • A = negative, B = positive, C = positive
  • A = negative, B = negative, C = positive
  • A = positive, B = negative, C = negative
  • A = positive, B = positive, C = negative

Pregunta 90

Pregunta
What are the results of the motility test pictured?
Image:
3_motilitylabeled (image/jpg)
Respuesta
  • A = non-motile, B = motile, C = motile
  • A = non-motile, B = non-motile, C = motile
  • A = motile, B =motile, C = non-motile
  • A = motile, B = non-motile, C = non-motile

Pregunta 91

Pregunta
What are the results of the sensitivity test pictured?
Image:
3_opto_srpn-srvi (image/jpg)
Respuesta
  • A = sensitive, B = resistant
  • A = resistant, B = sensitive

Pregunta 92

Pregunta
How do you calculate the titer of phage in the original sample given the number of plaques and the dilution of the plate?
Respuesta
  • # of plaques x dilution x 10^-1 = _________ PFU/mL
  • # of plaques x dilution x 10^1= __________ PFU/mL

Pregunta 93

Pregunta
What kind of hemolysis is pictured?
Image:
3_Gamma_hemolysis_M_luteus_Blood_Agar (image/jpg)
Respuesta
  • Gamma
  • Alpha
  • Beta

Pregunta 94

Pregunta
Which bacteria is cogulase positive and manitol slat agar positive?
Respuesta
  • Staphylococcus aureus
  • Staphylococcus epiderminis
  • Micrococcus roseus
  • Micrococcus luteus

Pregunta 95

Pregunta
Which bacteria is coagulase negative and manitol salt agar negative?
Respuesta
  • Staphylococcus epidermidis
  • Stapylococcus aureus
  • Micrococcus roseus
  • Micrococcus luteus

Pregunta 96

Pregunta
What bacteria is positive for nitrate reduction and rose for pigment production?
Respuesta
  • Micrococcus roseus
  • Micrococcus luteus
  • Staphylococcus aureus
  • Staphylococcus epidermidis

Pregunta 97

Pregunta
What bacteria is negative for nitrate reduction and yellow for pigment production?
Respuesta
  • Micrococcus luteus
  • Micrococcus roseus
  • Staphylococcus aureus
  • Staphylococcus epidermidis

Pregunta 98

Pregunta
How is Enterococcus faecalis differentiated from other alpha hemolytic streptococcus?
Respuesta
  • It is positive for bile esculin
  • It is negative for bile esculin

Pregunta 99

Pregunta
Which Streptococcus bacteria is optochin resistant?
Respuesta
  • Streptococcus mitis
  • Streptococcus pneumoniae
  • Streptococcus pyogenes
  • Group C streptococcus

Pregunta 100

Pregunta
Which streptococcus bacteria is optochin sensitive?
Respuesta
  • Streptococcus pneumoniae
  • Streptococcus mitis
  • Streptococcus pyogenes
  • Group C Streptococcus

Pregunta 101

Pregunta
Which bacteria is bacitration resistant?
Respuesta
  • Group C Streptococcus
  • Streptococcus pyogenes
  • Streptococcus pneumoniae
  • Streptococcus mitis

Pregunta 102

Pregunta
Which bacteria is bacitracin sensitive?
Respuesta
  • Streptococcus pyogenes
  • Streptococcus mitis
  • Streptococcus pneumoniae
  • Group C Streptococcus

Pregunta 103

Pregunta
Which bacteria are alpha hemolytic?
Respuesta
  • Streptococcus mitis
  • Streptococcus pneumoniae
  • Streptococcus pyogenes
  • Group C Streptococcus

Pregunta 104

Pregunta
Which bacteria are beta hemolytic?
Respuesta
  • Streptococcus mitis
  • Streptococcus pneumoniae
  • Streptococcus pyogenes
  • Group C Streptococcus

Pregunta 105

Pregunta
What hemolysis is pictured?
Image:
Staph_hemolysis_Blood_Agar (image/jpg)
Respuesta
  • A= alpha, B = beta, C = gamma
  • A = beta, B = alpha, C = gamma
  • A = gamma, B = alpha, C = beta
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