Microbiology Test 1 2nd Year PMU

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Microbiology Test 1 2nd Year PMU
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Resumen del Recurso

Pregunta 1

Pregunta
Name the components of nutrient media
Respuesta
  • Glucose Broth
  • Meat-Peptone Broth
  • Meat-Peptone agar
  • Blood Agar

Pregunta 2

Pregunta
Most of the bacterial colonies are visualized after:
Respuesta
  • 20 minutes
  • 2 hours
  • 1-2 days
  • 1 week

Pregunta 3

Pregunta
According to their cell wall structures bacteria divide into
Respuesta
  • Aerobes and Anarobes
  • Prokaryotes and Eukaryotes
  • Gram (+) and Gram (-)
  • Prototrophs and Auxotrophs

Pregunta 4

Pregunta
Extra chromosomal genetic elements in bacteria are
Respuesta
  • Mesosomes
  • Phagosomes
  • Transposons
  • Plasmids

Pregunta 5

Pregunta
How many phases have growth curve of bacteria? Ans: [blank_start]4[blank_end] Name them: a/ [blank_start]Lag[blank_end] Phase b/ [blank_start]Log[blank_end] Phase c/ [blank_start]Stationary[blank_end] Phase d/ [blank_start]Decline[blank_end] Phase
Respuesta
  • 4
  • Lag
  • Log
  • Stationary
  • Decline

Pregunta 6

Pregunta
Sex-pili of bacteria helps for their
Respuesta
  • Movement
  • Conjugation
  • Nutrition
  • Protection

Pregunta 7

Pregunta
Name the three shapes of bacteria: a/ [blank_start]Round[blank_end] ([blank_start]Cocci[blank_end]) b/ [blank_start]Rods[blank_end] c/ [blank_start]Spiral[blank_end] ([blank_start]Spirilli[blank_end])
Respuesta
  • Round
  • Cocci
  • Rods
  • Spiral
  • Spirilli

Pregunta 8

Pregunta
What is the most resistant to an unappropiate conditions bacterial form?
Respuesta
  • Vegetative
  • Spore

Pregunta 9

Pregunta
Which of the following bacterial structures prevents phagocytosis?
Respuesta
  • Cytoplasm
  • Peptidoglycan
  • Capsule
  • Nuclues

Pregunta 10

Pregunta
Are flagellas an obligatory structure of bacterium?
Respuesta
  • True
  • False

Pregunta 11

Pregunta
What chemical is responsible for the acid-fast property of mycobacteria? Ans: [blank_start]Mycolic acids[blank_end]
Respuesta
  • Mycolic acids

Pregunta 12

Pregunta
Name two bacteria that have capsules: a/ [blank_start]E. coli[blank_end] b/ [blank_start]Salmonella[blank_end]
Respuesta
  • E. coli
  • Salmonella

Pregunta 13

Pregunta
In microbiology what is the most commonly used objective Ans: [blank_start]Oil[blank_end] Explain: This is because [blank_start]oil[blank_end] unlike other liquids like water has very [blank_start]little light refraction[blank_end]
Respuesta
  • Oil
  • oil
  • little light refraction

Pregunta 14

Pregunta
What is the name of staining method for Corynebacterium diphtheriae?
Respuesta
  • Gram
  • Moller
  • Zhiehl-Neelsen
  • Neisser

Pregunta 15

Pregunta
Gram (+) bacteria are stained in [blank_start]blue[blank_end] colour and Gram (-) in [blank_start]red[blank_end] colour
Respuesta
  • blue
  • red

Pregunta 16

Pregunta
The main taxonomic category for bacteria is
Respuesta
  • Genus
  • Species
  • Family
  • Ordo

Pregunta 17

Pregunta
According Gram, the Gram (-) bacteria are stained in [blank_start]red[blank_end] colour
Respuesta
  • red

Pregunta 18

Pregunta
The unit for bacterial size is:
Respuesta
  • milimeter
  • micrometer
  • nanometer
  • angstrom

Pregunta 19

Pregunta
Vibrions are curved bacteria with
Respuesta
  • 4-6 curves
  • many curves
  • one curve
  • two curves

Pregunta 20

Pregunta
Ziehl-Neelsen is a staining procedure in:
Respuesta
  • encapsulated bacteria
  • spore-forming bacteria
  • acid-fast bacteria
  • motile bacteria

Pregunta 21

Pregunta
Bacterial cytoplasm contains:
Respuesta
  • capsule
  • ribosomes
  • nucleotide
  • peptidoglycan
  • pili
  • inclusions

Pregunta 22

Pregunta
Name the purposes of simple staining: a/ Determine cell [blank_start]shape[blank_end] b/ Determine cell [blank_start]size[blank_end] c/ Determine cell [blank_start]arrangement[blank_end]
Respuesta
  • shape
  • size
  • arrangement

Pregunta 23

Pregunta
The main staining procedure that detect morphology of bacteria is:
Respuesta
  • Loeffler
  • Neisser
  • Gram
  • Pfeiffer

Pregunta 24

Pregunta
The name Baccilus antracis defines certain bacterial
Respuesta
  • Genus
  • Species
  • Class

Pregunta 25

Pregunta
Is it true: Bacterial spores are formed in unfavorable conditions and are means of multiplying
Respuesta
  • True
  • False

Pregunta 26

Pregunta
Staining according Victoria blau is a [blank_start]simple[blank_end] method
Respuesta
  • simple
  • complex

Pregunta 27

Pregunta
Which of the following organelles are essential for bacteria?
Respuesta
  • cytoplasm membrane
  • pili
  • capsule
  • flagella
  • nucleoid
  • ribosomes

Pregunta 28

Pregunta
What are the curved bacteria?
Respuesta
  • staphylococci
  • helicobacter
  • vibrions
  • bacilli

Pregunta 29

Pregunta
Diplococci are [blank_start]round[blank_end] shaped bacteria that are arranged in [blank_start]pairs[blank_end]
Respuesta
  • round
  • pairs

Pregunta 30

Pregunta
Staphylococci are round shaped bacteria arranged in [blank_start]clusters[blank_end]
Respuesta
  • clusters

Pregunta 31

Pregunta
The volutine bodies are inclusions in the cytoplasm of:
Respuesta
  • menigococci
  • diptheria bacteria
  • genococci
  • pneumococci

Pregunta 32

Pregunta
Where is lipopolysaccaride situated?
Respuesta
  • in the cell wall of Gram (+) bacteria
  • in the cell wall of Gram (-) bacteria
  • in the both groups

Pregunta 33

Pregunta
Name at least two rod-shaped bacteria a/ [blank_start]Bacillus anthracis[blank_end] b/ [blank_start]Clostridium tetani[blank_end]
Respuesta
  • Bacillus anthracis
  • Clostridium tetani

Pregunta 34

Pregunta
How many spores form one bacteria?
Respuesta
  • one
  • two
  • three
  • more than one

Pregunta 35

Pregunta
Loefler technique stain bacteria in [blank_start]blue[blank_end] colour
Respuesta
  • blue

Pregunta 36

Pregunta
The causative agent of tuberculosis is stained in red color, using [blank_start]Ziehl[blank_end] [blank_start]Neelsen[blank_end] (name the staining method)
Respuesta
  • Ziehl
  • Neelsen

Pregunta 37

Pregunta
What are the two purposes of heat fixation a/ [blank_start]kill[blank_end] organisms b/ allows stain to be [blank_start]absorbed[blank_end]
Respuesta
  • kill
  • absorbed

Pregunta 38

Pregunta
Each bacteria has two names and they define its
Respuesta
  • family and genus
  • family and kingdom
  • genus and species

Pregunta 39

Pregunta
Bacteria cell is
Respuesta
  • eukaryotic
  • prokaryotic

Pregunta 40

Pregunta
Which of the following is NOT found in the cell wall of gram (-) bacteria?
Respuesta
  • Lipid A
  • Teichoic acids
  • O-antigen
  • peptidoglycan

Pregunta 41

Pregunta
Name the different types of bacteria pili and what are their function a/ [blank_start]common[blank_end] and its function is [blank_start]adhesion[blank_end] b/ [blank_start]sex[blank_end] and its function is [blank_start]conjugation[blank_end]
Respuesta
  • common
  • adhesion
  • sex
  • conjugation

Pregunta 42

Pregunta
Loeffler stain requires
Respuesta
  • Methylene blue
  • Ziehl fuschin
  • Gentian violet
  • Iodine solution

Pregunta 43

Pregunta
Which of the following methods of staining are complex
Respuesta
  • Pfeiffer
  • Neisser
  • Klett
  • Loeffler

Pregunta 44

Pregunta
Bacterium Diphteri and its structure : [blank_start]Volutine granule[blank_end]
Respuesta
  • Volutine granule

Pregunta 45

Pregunta
The volutine granules of Bacterium Diphteri are visualized by : [blank_start]Neisser[blank_end] staining
Respuesta
  • Neisser

Pregunta 46

Pregunta
The following structures are localized in the cytoplasm:
Respuesta
  • Spores
  • Nucleoids
  • Flagellum
  • Capsules

Pregunta 47

Pregunta
Based on their cellular structure bacteria are divided into:
Respuesta
  • Cocci and rods
  • Gram + and Gram –
  • Spares and spiral
  • Aerobes and anaerobe

Pregunta 48

Pregunta
Peptides Glycan polymer are found only in :
Respuesta
  • Bacterial cell
  • Animal cell
  • In viruses

Pregunta 49

Pregunta
Chemical structure of the capsule is composed of :
Respuesta
  • Complex polysaccharides
  • Vitamins
  • Polypeptides

Pregunta 50

Pregunta
Sporulation appears to be :
Respuesta
  • An initiation of reproduction of bacterial cell
  • A reaction of conditions that are unfavorable for normal growth
  • A status to resist desiccation and other harmful conditions
  • a & b
  • b & c

Pregunta 51

Pregunta
The complete killing or removal of all microorganisms including bacteria spores is called :
Respuesta
  • Sterilization
  • Disinfection
  • Antisepsis
  • Sepsis

Pregunta 52

Pregunta
What is the color of the simple method of Pfeiffer staining : [blank_start]red[blank_end]
Respuesta
  • red

Pregunta 53

Pregunta
Pfeiffer technical staining makes use of the following :
Respuesta
  • Methylene blue
  • Carbon Fuchsine
  • Neutral red
  • Gentian violet

Pregunta 54

Pregunta
The cell wall of gram (+) bacteria is composed mainly :
Respuesta
  • Hitine (typical for plants)
  • Peptidoglycan (90%)
  • Cellulose
  • Sugars

Pregunta 55

Pregunta
Which of the following bacteria have peptidoglycan layer:
Respuesta
  • Gram (+) cocci
  • Gram (-) bacteria
  • Both of them
  • None above

Pregunta 56

Pregunta
Cellular DNA is concentrated in (what place) :
Respuesta
  • Cytoplasm as a nucleoid
  • Mitochondria
  • Plasmids as an extra chromosomal genetic material
  • Ribosomes

Pregunta 57

Pregunta
The spores stain by Moller are :
Respuesta
  • Blue color
  • Red color
  • Black color
  • Yellow color

Pregunta 58

Pregunta
Which structure is NOT protecting bacteria from phagocytosis from leukocytes:
Respuesta
  • Spores
  • Capsule
  • Flagella
  • Ribosome

Pregunta 59

Pregunta
Name two simple (basic) stains a/ [blank_start]Loeffler[blank_end] b/ [blank_start]Pfeiffer[blank_end]
Respuesta
  • Loeffler
  • Pfeiffer

Pregunta 60

Pregunta
The concentration of ethyl alcohol as an antiseptic for disinfection is :
Respuesta
  • 95%
  • 70%
  • 50%
  • 30%

Pregunta 61

Pregunta
Bacterial nucleoid consists of :
Respuesta
  • No chromosomes
  • Only one chromosome
  • Several chromosomes

Pregunta 62

Pregunta
What is the name of staining procedure for bacterial capsule:
Respuesta
  • Gram stain
  • Loeffler stain
  • Klett stain
  • Moller stain

Pregunta 63

Pregunta
Lipopolysaccharide can be found in large amount :
Respuesta
  • Gram (+)
  • Gram (-)
  • Both of them
  • None of above

Pregunta 64

Pregunta
Complex method for bacterial staining are :
Respuesta
  • Loeffler
  • Pfeiffer
  • Gram
  • Ziehl-Neelsen

Pregunta 65

Pregunta
Bacterial antibiotic resistance is frequently covered by :
Respuesta
  • Tempered bacteriophage
  • R-plasmids
  • Lipid bacteriophage
  • Intron

Pregunta 66

Pregunta
What constitutes a bacterial culture : a/ [blank_start]Solute[blank_end] b/ dry [blank_start]agar[blank_end] c/ [blank_start]broth[blank_end]
Respuesta
  • Solute
  • agar
  • broth

Pregunta 67

Pregunta
Which one is a liquid medium for bacterial culture:
Respuesta
  • Blood agar
  • Glucose broth
  • Both of them
  • None above

Pregunta 68

Pregunta
Anaerobes are bacteria that can tolerate high oxygen concentration
Respuesta
  • True
  • False

Pregunta 69

Pregunta
What bacterial physiologist studies :
Respuesta
  • Morphology of bacteria
  • Bacterial structure
  • Bacterial growth
  • Bacterial nutrient

Pregunta 70

Pregunta
70% ethanol is :
Respuesta
  • Sterilizing agent
  • Antiseptic
  • Antibiotic
  • None is true

Pregunta 71

Pregunta
Which of the following is most important structure related to microbial attachment to cells :
Respuesta
  • Flagellum
  • Plasmid
  • Peptidoglycan
  • Ordinary Pilli

Pregunta 72

Pregunta
Which statement about genetic transfer among bacteria requires cell to cell contact:
Respuesta
  • Process of conjugation
  • Process of transduction
  • Process of transcription
  • Process of auto combination
  • Process of transformation

Pregunta 73

Pregunta
Which of the following related to endotoxin is not true:
Respuesta
  • Endotoxins are secreted from living cells
  • Endotoxins are part of cellular gram (-) bacteria
  • They are secreted only when the cell is disrupted
  • They can be linked to meningococcemia
  • They are produced by gram(-) microorganisms
  • They cause fever

Pregunta 74

Pregunta
What cause (hint) to be pink :
Respuesta
  • Carbon Fuchsin
  • Iodine solution
  • Crystal violet
  • Neisser A

Pregunta 75

Pregunta
Gram (-) bacteria differ from Gram (+) ones because the posses :
Respuesta
  • Lipopolysaccharide
  • Peptidoglycan
  • Only cytoplasmic membrane
  • Lipoteichoic acid

Pregunta 76

Pregunta
The auto-sterilisation requires the following :
Respuesta
  • 1:30 hours at 160 C
  • 134 C for 10-15 minutes

Pregunta 77

Pregunta
The chlorine is :
Respuesta
  • Antiseptic
  • Disinfectant
  • Chemical agent for sterilization
  • All of above

Pregunta 78

Pregunta
Concerning bacterial cell division everything is true except that :
Respuesta
  • All bacteria double their numbers every 10 hours
  • Bacterial generation times are influenced by environmental conditions
  • Two bacterial cells following division are genetically identical and they are once

Pregunta 79

Pregunta
How does high temperature cause cell death?
Respuesta
  • Oxidative damage
  • High level of electrolytes
  • Protein denature
  • All of above

Pregunta 80

Pregunta
How are the actions of phenols and alcohols similar :
Respuesta
  • Both denature proteins
  • Both oxidize DNA
  • Both inhibit nucleic acid synthesis
  • All of above

Pregunta 81

Pregunta
What is pasteurization :
Respuesta
  • A method that disinfects the instruments
  • Sterilizes food
  • A method that can be used on skin and mucus
  • All of above

Pregunta 82

Pregunta
Bacterial flagella are :
Respuesta
  • Composed of pillin
  • A floppy structure made of flagellin
  • Rigger structure made of flagellin

Pregunta 83

Pregunta
Name the complex nutrient media :
Respuesta
  • Glucose broth
  • Mid peptone broth
  • Mid peptone agar
  • Blood agar

Pregunta 84

Pregunta
Extra chromosomal genetic elements in bacteria are :
Respuesta
  • Mesosomes
  • Phagosomes
  • Transposomes
  • Bacteriophages ( or plasmids)

Pregunta 85

Pregunta
What part of the bacterial cell is most involved with Gram staining and why? Ans: [blank_start]Cell wall[blank_end] Explanation: Gram [blank_start]positive[blank_end] bacteria have a [blank_start]thick[blank_end] peptidoglycan cell [blank_start]wall[blank_end] that holds the stain [blank_start]better[blank_end] than the [blank_start]thin[blank_end] peptidoglycan gram [blank_start]negative[blank_end] cell [blank_start]wall[blank_end]
Respuesta
  • positive
  • thick
  • wall
  • better
  • thin
  • negative
  • wall
  • Cell wall

Pregunta 86

Pregunta
What is the purpose of heat during the acid-fast staining procedure? The heat [blank_start]drives[blank_end] the stain [blank_start]into[blank_end] the cells
Respuesta
  • drives
  • into

Pregunta 87

Pregunta
Name two curved bacteria: a. [blank_start]Vibrio Cholerae[blank_end] b. [blank_start]Helicobacteria[blank_end]
Respuesta
  • Vibrio Cholerae
  • Helicobacteria

Pregunta 88

Pregunta
Name the reagent used and state the purpose of each of the following a/ mordant: Reagent- [blank_start]Iodine[blank_end] solution, Function: form a [blank_start]complex[blank_end] between the crystal violet and [blank_start]iodine[blank_end] b/ primary stain: Reagent- [blank_start]Gentian Violet[blank_end], Function: increases the [blank_start]contrast[blank_end] of both gram-negative and gram-positive bacteria c/ decolorizer: Reagent- [blank_start]Alcohol[blank_end], Function: [blank_start]dehydrates[blank_end] the peptidoglycan layer d/ counterstain: Reagent- [blank_start]carbol fuschin[blank_end], Function: stains solution light [blank_start]red[blank_end] allowing Gram [blank_start]negative[blank_end] to be seen
Respuesta
  • Alcohol
  • Iodine
  • complex
  • iodine
  • Gentian Violet
  • contrast
  • dehydrates
  • carbol fuschin
  • red
  • negative

Pregunta 89

Pregunta
Round bacteria in chains are named [blank_start]streptococci[blank_end]
Respuesta
  • streptococci

Pregunta 90

Pregunta
What suggestions about endotoxins are true? a/ they are typical for [blank_start]Gram (-)[blank_end] b/ they act [blank_start]non-specific[blank_end] c/ their chemical structure is [blank_start]lipopolysaccarides[blank_end] d/ they are heat [blank_start]stable[blank_end]
Respuesta
  • Gram (-)
  • Gram (+)
  • non-specific
  • specific
  • lipopolysaccarides
  • proteins
  • stable
  • labile
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