Pregunta 1
Pregunta
Name the components of nutrient media
Respuesta
-
Glucose Broth
-
Meat-Peptone Broth
-
Meat-Peptone agar
-
Blood Agar
Pregunta 2
Pregunta
Most of the bacterial colonies are visualized after:
Respuesta
-
20 minutes
-
2 hours
-
1-2 days
-
1 week
Pregunta 3
Pregunta
According to their cell wall structures bacteria divide into
Pregunta 4
Pregunta
Extra chromosomal genetic elements in bacteria are
Respuesta
-
Mesosomes
-
Phagosomes
-
Transposons
-
Plasmids
Pregunta 5
Pregunta
How many phases have growth curve of bacteria?
Ans: [blank_start]4[blank_end]
Name them:
a/ [blank_start]Lag[blank_end] Phase
b/ [blank_start]Log[blank_end] Phase
c/ [blank_start]Stationary[blank_end] Phase
d/ [blank_start]Decline[blank_end] Phase
Respuesta
-
4
-
Lag
-
Log
-
Stationary
-
Decline
Pregunta 6
Pregunta
Sex-pili of bacteria helps for their
Respuesta
-
Movement
-
Conjugation
-
Nutrition
-
Protection
Pregunta 7
Pregunta
Name the three shapes of bacteria:
a/ [blank_start]Round[blank_end] ([blank_start]Cocci[blank_end])
b/ [blank_start]Rods[blank_end]
c/ [blank_start]Spiral[blank_end] ([blank_start]Spirilli[blank_end])
Respuesta
-
Round
-
Cocci
-
Rods
-
Spiral
-
Spirilli
Pregunta 8
Pregunta
What is the most resistant to an unappropiate conditions bacterial form?
Pregunta 9
Pregunta
Which of the following bacterial structures prevents phagocytosis?
Respuesta
-
Cytoplasm
-
Peptidoglycan
-
Capsule
-
Nuclues
Pregunta 10
Pregunta
Are flagellas an obligatory structure of bacterium?
Pregunta 11
Pregunta
What chemical is responsible for the acid-fast property of mycobacteria?
Ans: [blank_start]Mycolic acids[blank_end]
Pregunta 12
Pregunta
Name two bacteria that have capsules:
a/ [blank_start]E. coli[blank_end]
b/ [blank_start]Salmonella[blank_end]
Pregunta 13
Pregunta
In microbiology what is the most commonly used objective
Ans: [blank_start]Oil[blank_end]
Explain:
This is because [blank_start]oil[blank_end] unlike other liquids like water has very [blank_start]little light refraction[blank_end]
Respuesta
-
Oil
-
oil
-
little light refraction
Pregunta 14
Pregunta
What is the name of staining method for Corynebacterium diphtheriae?
Respuesta
-
Gram
-
Moller
-
Zhiehl-Neelsen
-
Neisser
Pregunta 15
Pregunta
Gram (+) bacteria are stained in [blank_start]blue[blank_end] colour and Gram (-) in [blank_start]red[blank_end] colour
Pregunta 16
Pregunta
The main taxonomic category for bacteria is
Respuesta
-
Genus
-
Species
-
Family
-
Ordo
Pregunta 17
Pregunta
According Gram, the Gram (-) bacteria are stained in [blank_start]red[blank_end] colour
Pregunta 18
Pregunta
The unit for bacterial size is:
Respuesta
-
milimeter
-
micrometer
-
nanometer
-
angstrom
Pregunta 19
Pregunta
Vibrions are curved bacteria with
Respuesta
-
4-6 curves
-
many curves
-
one curve
-
two curves
Pregunta 20
Pregunta
Ziehl-Neelsen is a staining procedure in:
Respuesta
-
encapsulated bacteria
-
spore-forming bacteria
-
acid-fast bacteria
-
motile bacteria
Pregunta 21
Pregunta
Bacterial cytoplasm contains:
Respuesta
-
capsule
-
ribosomes
-
nucleotide
-
peptidoglycan
-
pili
-
inclusions
Pregunta 22
Pregunta
Name the purposes of simple staining:
a/ Determine cell [blank_start]shape[blank_end]
b/ Determine cell [blank_start]size[blank_end]
c/ Determine cell [blank_start]arrangement[blank_end]
Pregunta 23
Pregunta
The main staining procedure that detect morphology of bacteria is:
Respuesta
-
Loeffler
-
Neisser
-
Gram
-
Pfeiffer
Pregunta 24
Pregunta
The name Baccilus antracis defines certain bacterial
Pregunta 25
Pregunta
Is it true: Bacterial spores are formed in unfavorable conditions and are means of multiplying
Pregunta 26
Pregunta
Staining according Victoria blau is a [blank_start]simple[blank_end] method
Pregunta 27
Pregunta
Which of the following organelles are essential for bacteria?
Respuesta
-
cytoplasm membrane
-
pili
-
capsule
-
flagella
-
nucleoid
-
ribosomes
Pregunta 28
Pregunta
What are the curved bacteria?
Respuesta
-
staphylococci
-
helicobacter
-
vibrions
-
bacilli
Pregunta 29
Pregunta
Diplococci are [blank_start]round[blank_end] shaped bacteria that are arranged in [blank_start]pairs[blank_end]
Pregunta 30
Pregunta
Staphylococci are round shaped bacteria arranged in [blank_start]clusters[blank_end]
Pregunta 31
Pregunta
The volutine bodies are inclusions in the cytoplasm of:
Respuesta
-
menigococci
-
diptheria bacteria
-
genococci
-
pneumococci
Pregunta 32
Pregunta
Where is lipopolysaccaride situated?
Pregunta 33
Pregunta
Name at least two rod-shaped bacteria
a/ [blank_start]Bacillus anthracis[blank_end]
b/ [blank_start]Clostridium tetani[blank_end]
Respuesta
-
Bacillus anthracis
-
Clostridium tetani
Pregunta 34
Pregunta
How many spores form one bacteria?
Respuesta
-
one
-
two
-
three
-
more than one
Pregunta 35
Pregunta
Loefler technique stain bacteria in [blank_start]blue[blank_end] colour
Pregunta 36
Pregunta
The causative agent of tuberculosis is stained in red color, using [blank_start]Ziehl[blank_end] [blank_start]Neelsen[blank_end] (name the staining method)
Pregunta 37
Pregunta
What are the two purposes of heat fixation
a/ [blank_start]kill[blank_end] organisms
b/ allows stain to be [blank_start]absorbed[blank_end]
Pregunta 38
Pregunta
Each bacteria has two names and they define its
Respuesta
-
family and genus
-
family and kingdom
-
genus and species
Pregunta 39
Pregunta
Bacteria cell is
Pregunta 40
Pregunta
Which of the following is NOT found in the cell wall of gram (-) bacteria?
Respuesta
-
Lipid A
-
Teichoic acids
-
O-antigen
-
peptidoglycan
Pregunta 41
Pregunta
Name the different types of bacteria pili and what are their function
a/ [blank_start]common[blank_end] and its function is [blank_start]adhesion[blank_end]
b/ [blank_start]sex[blank_end] and its function is [blank_start]conjugation[blank_end]
Respuesta
-
common
-
adhesion
-
sex
-
conjugation
Pregunta 42
Pregunta
Loeffler stain requires
Respuesta
-
Methylene blue
-
Ziehl fuschin
-
Gentian violet
-
Iodine solution
Pregunta 43
Pregunta
Which of the following methods of staining are complex
Respuesta
-
Pfeiffer
-
Neisser
-
Klett
-
Loeffler
Pregunta 44
Pregunta
Bacterium Diphteri and its structure : [blank_start]Volutine granule[blank_end]
Pregunta 45
Pregunta
The volutine granules of Bacterium Diphteri are visualized by : [blank_start]Neisser[blank_end] staining
Pregunta 46
Pregunta
The following structures are localized in the cytoplasm:
Respuesta
-
Spores
-
Nucleoids
-
Flagellum
-
Capsules
Pregunta 47
Pregunta
Based on their cellular structure bacteria are divided into:
Respuesta
-
Cocci and rods
-
Gram + and Gram –
-
Spares and spiral
-
Aerobes and anaerobe
Pregunta 48
Pregunta
Peptides Glycan polymer are found only in :
Respuesta
-
Bacterial cell
-
Animal cell
-
In viruses
Pregunta 49
Pregunta
Chemical structure of the capsule is composed of :
Respuesta
-
Complex polysaccharides
-
Vitamins
-
Polypeptides
Pregunta 50
Pregunta
Sporulation appears to be :
Respuesta
-
An initiation of reproduction of bacterial cell
-
A reaction of conditions that are unfavorable for normal growth
-
A status to resist desiccation and other harmful conditions
-
a & b
-
b & c
Pregunta 51
Pregunta
The complete killing or removal of all microorganisms including bacteria spores is called :
Respuesta
-
Sterilization
-
Disinfection
-
Antisepsis
-
Sepsis
Pregunta 52
Pregunta
What is the color of the simple method of Pfeiffer staining : [blank_start]red[blank_end]
Pregunta 53
Pregunta
Pfeiffer technical staining makes use of the following :
Respuesta
-
Methylene blue
-
Carbon Fuchsine
-
Neutral red
-
Gentian violet
Pregunta 54
Pregunta
The cell wall of gram (+) bacteria is composed mainly :
Pregunta 55
Pregunta
Which of the following bacteria have peptidoglycan layer:
Respuesta
-
Gram (+) cocci
-
Gram (-) bacteria
-
Both of them
-
None above
Pregunta 56
Pregunta
Cellular DNA is concentrated in (what place) :
Pregunta 57
Pregunta
The spores stain by Moller are :
Respuesta
-
Blue color
-
Red color
-
Black color
-
Yellow color
Pregunta 58
Pregunta
Which structure is NOT protecting bacteria from phagocytosis from leukocytes:
Respuesta
-
Spores
-
Capsule
-
Flagella
-
Ribosome
Pregunta 59
Pregunta
Name two simple (basic) stains
a/ [blank_start]Loeffler[blank_end]
b/ [blank_start]Pfeiffer[blank_end]
Pregunta 60
Pregunta
The concentration of ethyl alcohol as an antiseptic for disinfection is :
Pregunta 61
Pregunta
Bacterial nucleoid consists of :
Respuesta
-
No chromosomes
-
Only one chromosome
-
Several chromosomes
Pregunta 62
Pregunta
What is the name of staining procedure for bacterial capsule:
Respuesta
-
Gram stain
-
Loeffler stain
-
Klett stain
-
Moller stain
Pregunta 63
Pregunta
Lipopolysaccharide can be found in large amount :
Respuesta
-
Gram (+)
-
Gram (-)
-
Both of them
-
None of above
Pregunta 64
Pregunta
Complex method for bacterial staining are :
Respuesta
-
Loeffler
-
Pfeiffer
-
Gram
-
Ziehl-Neelsen
Pregunta 65
Pregunta
Bacterial antibiotic resistance is frequently covered by :
Respuesta
-
Tempered bacteriophage
-
R-plasmids
-
Lipid bacteriophage
-
Intron
Pregunta 66
Pregunta
What constitutes a bacterial culture :
a/ [blank_start]Solute[blank_end]
b/ dry [blank_start]agar[blank_end]
c/ [blank_start]broth[blank_end]
Pregunta 67
Pregunta
Which one is a liquid medium for bacterial culture:
Respuesta
-
Blood agar
-
Glucose broth
-
Both of them
-
None above
Pregunta 68
Pregunta
Anaerobes are bacteria that can tolerate high oxygen concentration
Pregunta 69
Pregunta
What bacterial physiologist studies :
Respuesta
-
Morphology of bacteria
-
Bacterial structure
-
Bacterial growth
-
Bacterial nutrient
Pregunta 70
Pregunta
70% ethanol is :
Respuesta
-
Sterilizing agent
-
Antiseptic
-
Antibiotic
-
None is true
Pregunta 71
Pregunta
Which of the following is most important structure related to microbial attachment to cells :
Respuesta
-
Flagellum
-
Plasmid
-
Peptidoglycan
-
Ordinary Pilli
Pregunta 72
Pregunta
Which statement about genetic transfer among bacteria requires cell to cell contact:
Pregunta 73
Pregunta
Which of the following related to endotoxin is not true:
Respuesta
-
Endotoxins are secreted from living cells
-
Endotoxins are part of cellular gram (-) bacteria
-
They are secreted only when the cell is disrupted
-
They can be linked to meningococcemia
-
They are produced by gram(-) microorganisms
-
They cause fever
Pregunta 74
Pregunta
What cause (hint) to be pink :
Respuesta
-
Carbon Fuchsin
-
Iodine solution
-
Crystal violet
-
Neisser A
Pregunta 75
Pregunta
Gram (-) bacteria differ from Gram (+) ones because the posses :
Pregunta 76
Pregunta
The auto-sterilisation requires the following :
Respuesta
-
1:30 hours at 160 C
-
134 C for 10-15 minutes
Pregunta 77
Pregunta
The chlorine is :
Pregunta 78
Pregunta
Concerning bacterial cell division everything is true except that :
Respuesta
-
All bacteria double their numbers every 10 hours
-
Bacterial generation times are influenced by environmental conditions
-
Two bacterial cells following division are genetically identical and they are once
Pregunta 79
Pregunta
How does high temperature cause cell death?
Pregunta 80
Pregunta
How are the actions of phenols and alcohols similar :
Pregunta 81
Pregunta
What is pasteurization :
Pregunta 82
Pregunta
Bacterial flagella are :
Pregunta 83
Pregunta
Name the complex nutrient media :
Respuesta
-
Glucose broth
-
Mid peptone broth
-
Mid peptone agar
-
Blood agar
Pregunta 84
Pregunta
Extra chromosomal genetic elements in bacteria are :
Pregunta 85
Pregunta
What part of the bacterial cell is most involved with Gram staining and why?
Ans: [blank_start]Cell wall[blank_end]
Explanation: Gram [blank_start]positive[blank_end] bacteria have a [blank_start]thick[blank_end] peptidoglycan cell [blank_start]wall[blank_end] that holds the stain [blank_start]better[blank_end] than the [blank_start]thin[blank_end] peptidoglycan gram [blank_start]negative[blank_end] cell [blank_start]wall[blank_end]
Respuesta
-
positive
-
thick
-
wall
-
better
-
thin
-
negative
-
wall
-
Cell wall
Pregunta 86
Pregunta
What is the purpose of heat during the acid-fast staining procedure?
The heat [blank_start]drives[blank_end] the stain [blank_start]into[blank_end] the cells
Pregunta 87
Pregunta
Name two curved bacteria:
a. [blank_start]Vibrio Cholerae[blank_end]
b. [blank_start]Helicobacteria[blank_end]
Respuesta
-
Vibrio Cholerae
-
Helicobacteria
Pregunta 88
Pregunta
Name the reagent used and state the purpose of each of the following
a/ mordant: Reagent- [blank_start]Iodine[blank_end] solution, Function: form a [blank_start]complex[blank_end] between the crystal violet and [blank_start]iodine[blank_end]
b/ primary stain: Reagent- [blank_start]Gentian Violet[blank_end], Function: increases the [blank_start]contrast[blank_end] of both gram-negative and gram-positive bacteria
c/ decolorizer: Reagent- [blank_start]Alcohol[blank_end], Function: [blank_start]dehydrates[blank_end] the peptidoglycan layer
d/ counterstain: Reagent- [blank_start]carbol fuschin[blank_end], Function: stains solution light [blank_start]red[blank_end] allowing Gram [blank_start]negative[blank_end] to be seen
Respuesta
-
Alcohol
-
Iodine
-
complex
-
iodine
-
Gentian Violet
-
contrast
-
dehydrates
-
carbol fuschin
-
red
-
negative
Pregunta 89
Pregunta
Round bacteria in chains are named [blank_start]streptococci[blank_end]
Pregunta 90
Pregunta
What suggestions about endotoxins are true?
a/ they are typical for [blank_start]Gram (-)[blank_end]
b/ they act [blank_start]non-specific[blank_end]
c/ their chemical structure is [blank_start]lipopolysaccarides[blank_end]
d/ they are heat [blank_start]stable[blank_end]
Respuesta
-
Gram (-)
-
Gram (+)
-
non-specific
-
specific
-
lipopolysaccarides
-
proteins
-
stable
-
labile