DISAGGREGATION OF ANIMAL TISSUES

Nodos de los diagramas

• DISAGGREGATION OF ANIMAL TISSUES

• PRIMARY EX-PLANT  TECHNIQUE

• MECHANICAL DISAGGGREGATION

• ENZYMATIC DISSAGGREGATION

• EX-PLANT

• OUTGROWTH

• TRANSFER

• 2ndry EXPLANT  CULTURE

•     COLD TRYPSIN

• COLLAGENASE

• WARM TRYPSIN

• OVERNIGHT,SHORT,COLD INCUBATON

• LONG INCUBATION,REPEAT SAMPELING

• LONG INCUBATION , COMPLETE MEDIUM

• CENTRIFUGE

• RESUSPENDED AND SEED

• DISPERSED PRIMARY CULTURE

• SUBCULTURE

• CELL LINE