API 20E results and interpretations

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Year 2 BIO258 - Microbes and Man Fichas sobre API 20E results and interpretations, creado por Sophie Byrne el 14/11/2018.
Sophie Byrne
Fichas por Sophie Byrne, actualizado hace más de 1 año
Sophie Byrne
Creado por Sophie Byrne hace casi 6 años
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ONPG test Beta galactosidase digests ONPG to give ortho-nitrophenol Colourless --> yellow
ADH Arginine --> citruline + NH3 pH increase Yellow --> red/orange Phenol red indicator
LDC Lysine --> cadaverine pH increase Yellow --> red/orange Phenol red indicator
ODC Ornithine --> putriscine pH increase Yellow --> red/orange Phenol red indicator
CIT Citrate --> alkaline ammonium salt pH increase Bromothymol blue indicator --> blue
H2S Black deposit/thin line as ferrous sulfide produced
URE Degrades urea --> NH3 Increases pH Yellow --> orange/red
TDA Yellow --> dark red-brown Ferric hydrazone produced
IND Kovac's reagent turns pink if indole (product of 1st reaction) is present
VP Acetoin reacts w/ VP1, VP2, and creatine to give red colour
GEL Gelatinase degrades gelatine into a liquid, causing the black pigment (India ink) to diffuse
Others Fermentation/oxidation Blue --> yellow
OX Cytochrome oxidase oxidises cytochrome, releasing...
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