Bacillus - Corynebacterium

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Microbiology - Bacteriology Fichas sobre Bacillus - Corynebacterium, creado por smrrc el 01/07/2015.
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Spore-forming aerobic gram positive bacilli Bacillus spp.
Non-spore forming aerobic gram positive bacilli Listeria Erysipelothrix Corynebacterium
Are all Bacillus species found in soil? YES!
Is Bacillus catalase positive or negative? POSITIVE!
Name the most common disease you can get from Bacillus cereus. Food poisoning mediated by toxin (Pwede ding eye & wound infection kung opportunistic)
Enumerate the two forms of food poisoning Diarrheal (24 hours) caused by eating meat & Emetic (10 hours) caused by fried rice Note: Both of them are self limiting
Type of hemolysis on agar plate produced by Bacillus cereus Beta hemolysis
How can you acquire anthrax? Through contact with anthrax infected animals or animal products. There is no human-to-human spread.
The causative agent of woolsorter's disease Bacillus anthracis
Three forms of anthrax Cutaneous Inhalation Gastrointestinal
Describe the disease progression of cutaneous anthrax (PVU) Starts as a PAPULE VESICULAR Depressed black necrotic ULCER *eschar*
Describe the pathology of inhalation anthrax Begins as "viral-like" Respiratory symptoms, fever, fatigue, myalgia leading to hypoxia Possibility of rhinorrhea/runny nose
This form of anthrax is sometimes seen as oropharyngeal ulcerations with cervical adenopathy and fever GI anthrax
Give some complications of anthrax Meningitis (5%) Coma, Death (1-6 days after exposure)
Does recovery from anthrax confer infection? Fortunately, yes!
Give some antibiotics used after anthrax exposure Ciprofloxacin Tetracycline
Describe the characteristic appearance of Bacillus colonies Medusa head appearance Non-hemolytic Catalase positive
Bacteria associated with meat processors and veterinarians Listeria monocytogenes
Is congenital transmission possible for Listeriosis? Sadly, yes. :(
Fill in the data for Listeria monocytogenes Appearance on Gram stain: Oxygen requirements: Spore formation: Acid-fast: Temperature requirements: Hemolytic pattern: Motility pattern: GP short rods or coccobacilli Aerobic Non-spore-forming Not acid fast either Grows at 4 degrees Beta hemolysis Umbrella pattern
Listeriosis is predominantly food-borne. Yes or No? Yes!
Agent of swine erysipelas and human erysipeloid Erysipelothrix rhusiopathiae
Small GP bacilli Singly, short chains, filaments Aerobic or FA Nonmotile Catalase (-) Erysipelothrix rhusiopathiae
Erysipelothrix is resistant to what kind of environment? Cold and alkaline
Cutaneous human erysipelothrix infection consisting of localized cellulitis after acquisition through the skin Erysipeloid (-pelas for animals) (-peloid for man) *sounds like keloid*
Specimen of choice for diagnosis of erysipelothrix infection Skin biopsy
Medium for cultivation of Erysipelothrix SBA with 5% to 10% CO2
Triple Sugar Iron Agar Slant for Erysipelothrix: H2s Positive or Negative? Positive!
Gram positive bacilli resembling "Chinese letters" and have a characteristic "dry colonial morphology" Corynebacterium spp.
Hallmark of corynebacterium disease Adherent membrane of the tonsils, pharynx, nose
5 Complications of corynebacterium infection (5 top organs) Heart: myocarditis Kidney and Liver: inflammation Brain: peripheral neuropathy Lungs: airway obstruction DEATH
How does corynebacterium invade the body? Colonizing and proliferating in the throat, creating pseudomembrane and producing an EXOTOXIN leading to cell death
Culture medium selective for Corynebacteirum diphtheriae (black to gray) Cystine-Tellurite Medium (CTBA)
Culture medium differential for Corynebacteirum diphtheriae (black with brown halos) Tinsdale Agar
Biochemical characteristics of Corynebacteirum diphtheriae CUN :: PNP Catalalse (+) Urease (-) Nitrate (+)
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