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Transcription
Descripción
Degree (PH1402 - Cell and Molecular Biology) Pharmacy Mapa Mental sobre Transcription, creado por Has Maj el 25/12/2015.
Sin etiquetas
pharmacology
pharmacy
biology
cell biology
transcription
pharmacy
ph1402 - cell and molecular biology
degree
Mapa Mental por
Has Maj
, actualizado hace más de 1 año
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Creado por
Has Maj
hace casi 9 años
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Resumen del Recurso
Transcription
What is Transcription?
A section of DNA is decoded to create proteins, to create RNA
Differences from DNA Replication
Does not use SSB proteins
Only small section of DNA strand is broken
The RNA nucelotides have different sugar and uracil replaces thymine
Also single stranded
No Helicase needed
What are the sequence of events?
Elongation
The RNA Polymerase causes the alpha subunit of ribose to phosphodiester bond with -OH on the 3rd carbon. A pyrophosphate is lost
The RNA polymerase breaks open the RNA strand at -10 box
Initiation
Eukaryotes
Uses TATA box
Uses TF's (DABFEH)
RNA Polymerase II
Prokaryotes
Uses -35, -10 and +1 Box
RNA Polymerase attaches to the promoter region covering from -35- +1
Termination
A terminator region is reached as a result a stem loop forms. The RNA polymerase gets tangled with the stem loop and broken off
They are palindromes and RNA base pair itself
Factor Row
After this RNA can go straight into translatin
Prokaryotes
Eukaryotes
Pre-RNA is formed and further spliced into RNA
There are three parts to this: Capping, Polyadenylation and Intron Splicing
Capping
Guanine bond joined by 5 - 5 prime tri phosphae bond on 5 prime end base of the pre-MRNA
The guanine is then methylated
Polyadenylation
Signal reached, cleaved 10-30 bases downstream, 250 adenine bases added
Intron Splicing
Uses snRNP
OH on 2 prime carbon nucleophilic attack on 5 prime end of intron
The 5 prime -OH attackes 3 prime intron end of intron and a lariat forms
Structure of RNA Polymerase
Made up of five subunits, sigma subunit can detach to create a four subunit core enzyme
Sigma subunit tells RNA polymerase where promoter region starts
Recursos multimedia adjuntos
cedbccb3-8876-4de6-bce1-11637f65b027 (image/png)
a15a7206-8331-4325-be11-245967186ba9 (image/png)
dc60d584-f93a-48f4-b192-079b8806cf8e (image/png)
e8f6dd89-fa87-4754-adaf-ac169cdb4d23 (image/png)
12af9207-ff61-4f54-bf1d-ede51fb00873 (image/png)
add2217c-1a84-481f-90a4-065d3ebc9f55 (image/png)
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