Created by Jumael Zafar
over 6 years ago
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Question | Answer |
Why do you need an ELISA test? | To test for whether a patient has an antibody or an antigen to a particular pathogen, or an allergy. |
What does ELISA stand for? | Enzyme-Linked Immunosorbent Assay |
What are the two types of ELISA? | Direct and indirect |
Direct ELISA is faster and easier | You need an antibody which is complementary to the antigen being tested for. There must be an enzyme attached. |
Step 1: | Add enzyme attached antibodies to a solution of patient's antigens. |
Step 2: | If they are complementary, they will bind forming an antigen-antibody complex. |
Step 3: | Wash the plate to remove any unbound antibodies. |
Step 4: | Add substrate solution which complementary to the enzyme, on the antibody. |
Step 5: | If any is present, it will form a enzyme substrate complex, changing color, so the result is positive. |
What is indirect ELISA? | It is very similar to direct but you need two antibodies, a primary and secondary. |
Whhaaaaaaaaaaat? | The primary antibody should be complementary to the tested antigen and the secondary antibody should have an enzyme attached and be able to bind to the primary. |
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