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Description
Flashcards by Emily Rowland-Rawson, updated more than 1 year ago
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Created by Emily Rowland-Rawson
about 6 years ago
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| Question | Answer |
| features of a compound light microscope | • two lenses, objective and eyepiece (both magnify) • the objective/eyepiece lens configuration allows for much higher magnification and reduced chromatic aberration compared to a simple light microscope |
| sample preparation: dry mount | solid specimens are viewed whole or sectioned into thin slices and placed under a cover slip |
| wet mount | specimens are suspended in a liquid and a cover slip is placed on at a angle living organisms can be viewed this way |
| squash slides | a wet mount is prepared then the cover slip is gently pressed down, as it can break this is good for soft samples |
| smear slides | the edge of a slide is used to smear a thin even coating of the sample onto another slide, then a cover slip is placed on top (good for blood) |
| transmission electron microscope | • a beam of electrons transmitted through a specimen and focused to produce an image • resolving power of 0.5 nm |
| scanning electron microscope | • a beam of electrons is transmitted across the surface of a specimen and the reflected electrons are collected • resolving power 3-10 nm (not as good as transmission) • produces 3D images |
| nucleolus | • an area within the nucleus that produces ribosomes. made of proteins and RNA. • RNA is used to make ribosomal RNA (rRNA) which then combines with proteins to form necessary ribosomes for protein synthesis. |
| mitochondria | • the site of the final stages of cellular respiration • the number of mitochondria in a cell indicates how much energy it uses |
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