2061205
Description
Flashcards by Laura Perry, updated more than 1 year ago
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Created by Laura Perry
almost 10 years ago
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| Question | Answer |
| What three things did Schwann and Schleiden say about cells? | -All living things consist of cells -New cells are formed from the division of old cells -Instructions for growth are passed on to new cells |
| What is the magnification and resolution of a light microscope? | x1500 magnification 200nm resolution |
| Which of TEM and SEM has a greater magnification? | TEM |
| What is the resolution of both TEM and SEM? | 0.2nm |
| What is the definition of magnification? | Th number of times greater an image is than the object |
| What is the definition of resolution? | The ability to distinguish two separate points that are distinct from each other. |
| Why is a stain required in light microscopy? | This allows the different parts of a cell to become distinct from each other and creates definition. |
| What stain is used in TEM and what will the image look like? | Electrons pass through the sample- the more dense the sample, the longer it will take. False colour is applied afterwards to define between the different parts and this produces a 2D image |
| Why is a gold stain used in a SEM? | The gold stain allows the electrons fired at the sample to 'bounce' off, creating a 3D image that shows the contours of the specimen. |
| What is the equation for magnification? | Magnification= Image/ Actual |
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