miRNAs

Question	Answer
miR-132	Scott: Regulates LTD, delays object recognition memory. NMDA -> CREB-P -> miR-132 expression -// p250GAP translation p250GAP inhibits Rac so now Rac in the presence of kalirin can activate PAK to promote polymerisation p250GAP interacts with GluN2B, GluN2BK, and PSD-95
miR-138	Activates APT1 -> depalmitoylation of synaptic proteins e.g. activator of RhoA. Negative regulator of spines Found in dendritic regions, along with miRNA biosynthesis proteins : argonaute 2, dicer, p-body components
miR-3120	Targets dynamin 3 involved in the endocytosis pathway
Alzheimer's Disease	Hsieh: b-CTF to drive AB production. Reduces EPSCs and occludes DHPG LTD. APP overexpression reduces GluA2 surface expression. APPMV doesn't. miRNAs may be involved in the altered processing of APP, leading to altered endocytosis (PICALM, Bin1 (amphiphysins)) proteins shown to be involved in AD.
PICK1	36 miRNAs shown to be involved in regulation of BAR domain needed for endocytosis
Ischaemia	Stress -> changed miRNA expression -> changed receptor expression
miR-16	Binds to arginine rich elements on mRNA 3UTR regions to mediate degradation of mRNA. UTR region same in many tissues
miRNA target identification	Expression profiling: memory task extract proteins, find what miRNAs Stem cells: create miRNA libraries from stem cells and add a gene to see the affect. 3UTR assays to determine miRNA binding to genes. Proteomics. TF OR PROTEIN?

Resource summary

	Created by Madeline Fielder over 10 years ago