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Jm's project

Description

개인과제 management
jeongminha90
Mind Map by jeongminha90, updated more than 1 year ago
jeongminha90
Created by jeongminha90 about 9 years ago
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Resource summary

Jm's project
  1. Rep.MECH
    1. Microarray
      1. Rep. specific candidates
        1. iPSCR
          1. PDR
            1. intermediate
              1. LCE3 family
            2. GO analysis
              1. GSEA
                1. Panther pie chart
              2. VDR ligands
                1. qRT-PCR confirm
                  1. Rpl7, Rsp14
                  2. Over during Rep. (2'MEF)
                    1. AP staining
                      1. ICC
                        1. qRT-PCR
                      2. 2'MEF prep.
                        1. Puro. selection
                          1. Stock freezing
                            1. Rep. Test
                          2. Rep2hiDP-AV
                            1. hSTEMCCA-AV
                              1. Amplification (293T)
                                1. Ultra centrifuge
                                2. Working test (CRL2097)
                                  1. Rep. efficiency
                                    1. ICC
                                    2. Protein synthesis
                                      1. Western Blot
                                  2. hOK/SM-AV
                                  3. ICTR-rep.
                                    1. Reference searching
                                    2. iPSeval
                                      1. EB diff.
                                        1. HSP0076 characterization
                                        2. Immunogenicity
                                          1. Monkey Fib culture
                                            1. Monkey iPSC generation
                                              1. Sendai virus
                                                1. Episomal vector
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