5893960
Description
Mind Map by manura_roy_vargh, updated more than 1 year ago
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Created by manura_roy_vargh
over 8 years ago
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Genetic
Engineering
- Procedure
- RFLP
- Applications
- DNA fingerprinting
- Disease Detection
- Genomic Mapping
- DNA fingerprinting
- PCR
- Step 2: Annealing of DNA Primers @ 55C
- Step 1:Denaturation @ 95C
- Step 3: Elongation of DNA @ 75C
- Step 2: Annealing of DNA Primers @ 55C
- Gel Electrophoresis
- Nucleic Acid Hybridisation
- RFLP
- Human Genome Project
- Goals
- Identify 25000 genes in human DNA
- Store the information on DNA seq in publicly available
domains
- Identify 25000 genes in human DNA
- Benefits
- Genetic Testing
- Pharmacogenomics
- Genetic Testing
- Ethical Implications
- Discrimination
- COmercialisation and Patenting
- Relaibility of Genetic Tests
- Discrimination
- Goals
- Tools
- DNA ligase
- Restriction Enzymes
- Vector
- DNA Library
- cDNA library
- Features
- Synthesis
- Features
- Genome Library
- Features
- Synthesis
- Features
- cDNA library
- DNA ligase
- Protein Production
- Process
- Step 5: Extraction & Purification
- Step 1: Isolation & Preparation of GOI
- Step 2: Construction of recombinant DNA
- Step 3: Transformation
- Step 4: Selection and Identification
- Blue/White colony screening
- Replica plating
- Blue/White colony screening
- Step 5: Extraction & Purification
- Human Growth Hormone
- Insulin
- Process
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