17860639
| Questão | Responda |
| Describe Aeromonas properties and characteristics | G + , straight rods. Ferments sugar. Needs 22-28°C for growth. Cat + and Ox +. Motile except A.salmonicida. Recistant to Abs, cold. |
| Describe Aeromonas on blood agar | A.hydrophila: medium/large, smooth, white, entire margin with hemolysis. A.salmonicida: medium, grey, w/o hemolysis. Some strains can have green/brown colouration on MPBA. |
| What does A.hydrophila cause? | ~the water lover~ Found in fresh water fish and amphibians. Causes ulcers and tail rot, septicemia, scale shedding, abdominal swelling in fish and red leg in frogs. Hemorrhages in gills and anal area. Can affect human causing gastroenteritis. |
| What does A.salmonicida cause? | Bloody skin ulcers- furunculus on salmons and gold fish |
| What do they all have in common? | CAT + |
| In what family is aeromonas? | Aeromonadaceae |
| Plesiomonas family | Enterobacteriaceae |
| Vibrio family | Vibrionaceae |
| Describe Vibrio- appearance, tests, toxins | G -, curved rods in chains with one polar flagellum . Halophile- 3.5% NaCl. Causes problems only in human skin and GIT. ~ Has enterotoxins and hemolysins Cat and Ox +, also Lac +. |
| Which plesiomonas strain is of pathogenic importance? | P.shigelloides. Causes gastroenteritis, septicemia, bloody diarrhea (enterotoxins) in fresh water fish, cattle, goat and dog. Not in human. |
| Which one of the 3 is associated with another important pathogenic bacterium and how? 1) A.salmonicida with salmonella 2)V.parahemolyticus with Staph.Hemolyticus 3) P.shigelloides with Shigella | 3) P.shigelloides with shigella sonnei, they share antigens, cross-reactions between them may occur. DIfference is that plesiomonas is Cat + and shigella Cat - |
| What is the species of vibrio that affects poultry? | V. Metchnikovii- gastroenteritis |
| Vibrio causes serious disease in cattle. What is it? | ONLY affects HUMANS, and poultry-1 strain |
| What is important for survival of V.parahemolyticus in environment? | Salt resistance since it's a salt sea pathogen |
| P.parahemolyticus causes | Mainly affects GIT after ingesting raw and infected seafood, incubation is 24h followed by explosive watery diarrhea, nausea and vomiting. Can last from 3-10 days depending on immunityof host. |
| What is cholera? | Infection of small intestine by V.cholerae. Causes diarrhea, dehydration and electrolyte imbalance in few hours. |
| How can you get infected by V.cholerae? | low hygiene, through bad food and WATER. |
| V.cholerae virulence factors | LPS, cholera toxin, vibrio pathogenicity islands with genes involved in toxin-coregulated pilus: Vibrio cholerae uses toxin-coregulated pili (TCP) to colonize the human intestine |
| How do you diagnose and treat cholera? | Stool test-dip stick test, also can be vaccinated, must test for Ab resistance before treatment. |
| What is specific medium for differentiation between the 2 species of vibrio? | TCBS- thio sulfate bile sucrose. V.cholerae: causes large yellow colonies & agar surrounding them V.parahemolyticus: only medium, dark green colonies |
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