2. Type 1 col. molecules are deposited and polymerised on the core
3. Small amounts of Type 2 and 3 col. molecules are incorporated into T1 col. fibrils
Elastic fibres
Accumulated EFs= yellow
Stain: Orcein (red-brown)
Loose CT Proper
Reticular fibres
Type III Col. fibrils embedded in glycoCHON matrix
Supporting network for cells in liver, glands and muscle tissue
Stain: Ag impregnation, PAS run
Reticular tissues- creates storm of hemopoietic organs
Ground Substance
GAGS
Hyaluronic acid
Sulphated GAGs
Proteoglycans
Multiadhesive
glycoproteins
Cartilage
Hyaline
Elastic
Fibrocartilage
Components
GAG
Decorin
Perlecan
Syndecan
Proper CT Cells
Dense collagen-
CT Proper
Aniline blue
T1 collagen form thick bundles in
reticular, skin, sclera and joint fascia
Collagen
Staining: HE= Pink,
Trichromes= named colour
T1: form fibres
T2: cartilage
T3: reticular fibre
Biosynthesis of T1
Anotações:
1. Collagen alpha chains are syn. in RER
2. Post-translational modification take place within the RER cisternae
3. Triple helix is formed with the help of hsp47
4. Intrachain and interchain H and S bonds form --> shape is influenced
5. Procollagen molecules --> Golgi --> Secretory vesicles --> Exocytosis
6. Fibrillogensis occurs