13592079
Descrição
Quiz por Jessica Bulley, atualizado more than 1 year ago
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Criado por Jessica Bulley
mais de 6 anos atrás
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Questão 1
Questão
Define Histopathology
Responda
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the study of changes in tissues caused by disease.
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the study of changes in cells caused by disease.
Questão 2
Questão
Select Three hazards associated with working in a Histopathology laboratory.
Responda
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- Chemical: fixatives, stains, chemicals
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– Biological: cross infection
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– Physical: sharps, radiation
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- Safety: slips, trips, falls
Questão 3
Questão
Explain what a fixative does
Responda
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preservation of tissue specimen
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decontaminates all viruses associated with the tissue specimen
Questão 4
Questão
List Six features of an ideal fixative
Responda
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• Will not swell or shrink tissue
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• Is economical
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• Has convenient shelf life and storage
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• Will be safe for user and environment
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• Will preserve tissue in life like manner
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• Will not add artefact material to tissue
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• Will add artefact material to tissue
Questão 5
Questão
Select Three fixative/s that can be used for Cytology smears?
Responda
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Spray fixed with aerosol of propanol and acetone+ Poly ethylene glycol- a water soluble wax
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Methanol
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Ethanol
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Xylene
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Sodium dihydrogen phosphate monohydrate 4g
Questão 6
Questão
Select Three physical agents that can be used to assist in the fixation of tissues.
Responda
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Microwave
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Heat
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Ultrasound
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Freeze
Questão 7
Questão
True or false: autolysis represents the changes to tissue that occur before fixation due to bacterial contamination.
Responda
- True
- False
Questão 8
Questão
What type of fixative is Neutral Buffered Formalin classed as?
Responda
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Universal fixative
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Fixative used for specific purposes
Questão 9
Questão
Why is Formalin Buffered to a neutral pH?
Responda
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because the further a fixative is from the pH of body tissues the more distortion you can get in the tissue at the cellular level.
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because the further a fixative is from the pH of body tissues the less distortion you can get in the tissue at the cellular level.
Questão 10
Questão
What is the function of xylene in tissue processing?
Responda
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To cut sections, the tissue has to be embedded in paraffin wax, but wax is not soluble in water or alcohol. However, it is soluble in a paraffin solvent called 'xylene'.
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Cleaning up the lab station to prevent contamination of other specimens and users of the facilities
Questão 11
Questão
Why is the histological section cut to approximately 5µm thick?
Responda
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- Sample needs to be thin enough for light microscopy (about 1 cell layer thick) - makes it easier to evaluate tissue and look at its morphology
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- Provides enough thickness to account for cell to cell ratio
Questão 12
Questão
List method/s used for the collection of Cytology samples.
Responda
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- Pap Smears, Endoscopy samples, some FNA (fine needle aspiration) specimens
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- Blood test, saliva test, sputum collection
Questão 13
Questão
Give an example of a Chemical Fixative that belongs to Aldehydes
Responda
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- Formaldehyde, Glutaraldehyde
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- Ethanol, Methanol
Questão 14
Questão
Give an example of a Chemical Fixative that belongs to Oxidising agents
Responda
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- Osmium tetroxide, Potassium Dichromate
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- Ethanol, Methanol
Questão 15
Questão
Give an example of a Chemical Fixative that belongs to Protein coagulants
Responda
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- Ethanol, Methanol
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- Mercuric Chloride, Picric acid
Questão 16
Questão
Give an example of a Chemical Fixative that belongs to Uncertain mechanism
Responda
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- Mercuric Chloride, Picric acid
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- Ethanol, Methanol
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