Questão 1
Questão
What is the main role of cytochrome aa3?
Responda
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Takes e- from ubiquinol and reduces oxygen to water
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Takes e- from ubiquinol and reduces oxygen to peroxide
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Takes e- from cytochrome c and reduces oxygen to peroxide
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Takes e- from cytochrome c and reduces oxygen to water
Questão 2
Questão
Complex IV contains how many subunits?
Questão 3
Questão
Complex IV doesn't transfer protons across the inner mitochondrial membrane
Questão 4
Questão
Which ions does it contain, and how many of each?
Responda
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2 Fe (in haeme groups) and 3 Cu (2 as a Cu-Cu binuclear centre, 1 as a binuclear Fe-Cu centre)
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2 Cu (in haeme groups) and 3 Fe (2 as a Fe-Fe binuclear centre, 1 as a binuclear Fe-Cu centre)
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2 Mg (in haeme groups) and 3 Cu (2 as a Cu-Cu binuclear centre, 1 as a binuclear Fe-Mg centre)
Questão 5
Questão
Cytochrome aa3 complex consists almost entirely of alpha helices
Questão 6
Questão
Put the following in order
A- Cyt c
B- CuA centre
C- Cyt a
D- Cyt a3-CuB centre
E - Water
•Transfer from cyt a3-CuB centre to oxygen
Responda
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A-B-C-D-E
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A-C-D-B-E
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A-C-B-D-E
Questão 7
Questão
How many protons are pumped by complex 4, and how many are used to make water (per Oxygen molecule)?
Questão 8
Questão
Which conformation of Complex IV has the low affinity for protons?
Questão 9
Questão
Protons bind to Complex IV during Conformation B
Questão 10
Questão
What happens during conformation B that lowers the Complex IV's affinity for protons?
Questão 11
Questão
No question, just look at it and learn it
Questão 12
Questão
ATP synthase is found on the inner mitochondrial membrane
Questão 13
Questão
ATP synthase is divided into two sections, F1 & F0
Which is peripheral and which is integral?
Questão 14
Questão
Name the function of ATP synthase
Responda
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ATP synthesis
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ATP hydrolysis
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ATP translocation
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Proton translocation
Questão 15
Questão
If ATP is high, and the proton gradient is low, what will Complex V do instead?
Responda
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Hydrolyse ATP
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Synthesise GTP
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Break itself down
Questão 16
Questão
Protons flow through F1 to power the ATP synthesis in F0
Questão 17
Questão
Name the subunits of F1
Questão 18
Questão
Which of the following subunit(s) are the largest in F1?
Responda
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Alpha & Beta
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Gamma & Delta
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Beta and Gamma
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Alpha and Epsilon
Questão 19
Questão
What is the role of the delta subunit in F1?
Questão 20
Questão
The epsilon subunit of F0 connects the gamma to the C subunit
Questão 21
Questão
F0 contains how many subunits?
Responda
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2- A & B
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3- A, B & C
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2- Alpha & Beta
Questão 22
Questão
The F1 subunit is...
Responda
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8nm long
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18nm long
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12nm long
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90nm wide
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19nm wide
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9nm wide
Questão 23
Questão
The Y subunit of F1 is made of 2 beta sheets that have been bent, but are bound at the base
Questão 24
Questão
When the gamma subunit is in contact with a beta subunit, the ATP catalysis site is in it's _ formation
Questão 25
Questão
A subunit contains ADP + Pi- what conformation is it in?
Questão 26
Questão
Whilst the subunit is replacing ATP with ADP + Pi, the gamma subunit remains in plance
Questão 27
Questão
What order do the three conformations work in?
Assume you start with the Loose formation
Responda
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Loose-Tight-Open
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Loose-Open-Tight
Questão 28
Questão
State the characteristics of the C subunit of F0
Responda
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Small
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Large
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Hydrophobic
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Hydrophilic
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Integral
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Peripheral
Questão 29
Questão
How many monomers make up the C subunit?
Questão 30
Questão
The C subunit contains 3 rings of monomer
Questão 31
Questão
Why is there a variance across species of the number of monomers in the C subunit?
Responda
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The more complex an organism, the more efficient the subunit
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The more complex an organism, the less efficient the subunit
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The larger the organism, the more subunits are required
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Mammals have 8, birds have 12, all lesser animals and prokaryotes have 14 or more
Questão 32
Questão
More subunits require more protons to move them, so more subunits is less efficient
Questão 33
Questão
How is the A subunit described?
Responda
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A proton half channel
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A proton channel
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A proton quarter channel
Questão 34
Questão
Protons passing through the A subunit...
Questão 35
Questão
Each subunit can hold 2 protons
Questão 36
Questão
The momevent of the protons around the C subunit powers what?
Responda
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The movement of the gamma subunit
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The conformational changes of the beta subunits
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The attachment of the alpha subunits
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The rotation of the delta subunit
Questão 37
Questão
The rotation of the F1 has been proved by attaching a microtubule filament to the F1 head and examining it under the microscope
Questão 38
Questão
Only the gamma subunit of the F1 complex rotates
Questão 39
Questão
How is the Alpha/Beta spheroid of F1 held in place?
Responda
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B1 subunit
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B2 subunit
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Epsilon subunit
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Delta subunit
Questão 40
Questão
For every _degree rotation, 1 molecule of ATP is produced
Questão 41
Questão
The mechanism of the gamma subunit causes an affinity change for ATP/ADP + Pi, driving the cycle