Questão 1
Questão
What is the main problem of protein translocation?
Responda
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Hydrophillic proteins must transverse two hydrophobic membranes and a hydrophillic space
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Proteins must be unfolded and refolded again
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Proteins must pass through an extremely crowded cytoplasm
Questão 2
Questão
Proteins to be secreted directly after translation have a peptidase sequence at the C-terminus
Questão 3
Questão
Which of the following parts of the peptidase sequence are closest to the N-terminus?
Questão 4
Questão
There are 1-2 basic residues in the signal sequence, but the hydrophobic sequence is variable in length
Questão 5
Questão
When a protein is transcribed with the Peptidase signal site, what protein binds to its hydrophobic region?
Questão 6
Questão
Ftsy transfers the protein into the pore
Questão 7
Questão
Which of the following provide the driving force for protein translocation across the membrane
Responda
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Protonmotive force
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FtsY's GTPase
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Protein translation
Questão 8
Questão
What binds to the nascent polypeptide if Post-Translational Translocation takes place?
Questão 9
Questão
Post-Translational Translocation sequences have a string of hydrophobic amino acids, same as in Translational Translocation
Questão 10
Questão
What is the role of Sec_ ?
Responda
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To keep the protein in solution till it binds to SecA at the pore
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To fold the protein on its way to the periplasmic more
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To stop the protein folding on its way to SecC
Questão 11
Questão
What forces are required to get a Post-Translational Translocation through the pore?
Responda
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GTPase of FtsY
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ATP hydrolysis by SecB
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Protonmotive Force
Questão 12
Questão
Once inside the required membrane, what protein removes the peptide sequence?
Responda
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Signal peptidase
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Signal lyase
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Signal Isomerase
Questão 13
Questão
The protein only folds after the signal sequence is cleaved