457002
Descrição
Quiz por gina_evans0312, atualizado more than 1 year ago
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Criado por gina_evans0312
quase 11 anos atrás
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Questão 1
Questão
Name the two methods of physical fragmentation
Responda
-
Shearing- filtering through a fine needed
-
Sonication
-
Acidification
-
Boiling
Questão 2
Questão
Non-random shearing involves the use of helicases
Responda
- True
- False
Questão 3
Questão
Which of the following cuts most frequently?
Responda
-
4 cutters
-
6 cutters
-
8 cutters
Questão 4
Questão
A 4 cutter cuts a 4 bp sequence, and so on
Responda
- True
- False
Questão 5
Questão
The staggered ends of two different enzymes can be ligated together, but afterwards cannot be cut by the same enzymes
Responda
- True
- False
Questão 6
Questão
When dealing with DNA-cutting enzymes, what does the word degenerate mean?
Responda
-
The ends they produce don't last long
-
They can cut more than one sequence
-
Where in the sequence they cut changes
Questão 7
Questão
What conditions are necessary for a partial digest?
Responda
-
Not enough time
-
Less enzyme
-
Wrong enzyme conditions
-
A four cutter
Questão 8
Questão
Why could a partial digest be done?
Responda
-
To get longer fragments and more likely preserve the gene
-
To save time
-
To prevent the DNA from degrading
Questão 9
Questão
Why is E.Coli most often used as a cloning vector?
Responda
-
It has a rapid generation time
-
Its DNA is easy to manipulate
-
Its DNA is easy to get hold of
-
They don't mutate much
Questão 10
Questão
Bacteriophages can be used to transplant vectors into E.Coli
Responda
- True
- False
Questão 11
Questão
How does M13 infect the male E.Coli?
Responda
-
Binds to pilus surface
-
Enters through bacterial pores
-
Direct injection of DNA
Questão 12
Questão
Once inside the cell, the ssDONA of M13...
Responda
-
Is convered to dsDNA that acts like a plasmid
-
Is integrated into the bacterial chromosome
-
Is cut up by restriction enzymes ans integrated into the bacterial chromosome
Questão 13
Questão
TEVEN and Lambda enter the bacteria directly through the pore
Responda
- True
- False
Questão 14
Questão
What is the point of the resistance genes in this Generation 1 plasmid
Image:
Gen_1_Plasmid (image/jpg)
Responda
-
Tetracycline is interrupted- E.Coli with no resistance have had the recombinant plasmid inserted
-
The recombinant DNA is inserted at the EcrR1 site
-
E.Coli with ampicillin resistance have taken up a version of the plasmid
Questão 15
Responda
-
The small subunit of LacZ
-
The large subunit of LacZ
-
Ampicillin
-
Tetracycline
Questão 16
Questão
Once the LacZ subunit has been assembled, what form does LacZ take?
Image:
Lac_Z (image/png)
Responda
-
Monomer
-
Dimer
-
Tetramer
-
Hexamer
Questão 17
Questão
For Gen 2 plasmid testing to work, the LacZ gene must be removed from the E.Coli, and a plasmid for the large LacZ subunit added
Responda
- True
- False
Questão 18
Questão
Why is the dish impregnated with X-Gal?
Responda
-
It goes from blue to white in the presence of LacZ
-
It goes from white to blue in the presence of LacZ
-
It goes from white to blue in the presence of the small subunit of LacZ
Questão 19
Questão
If the gene is successfully inserted, the colony will remain white
Responda
- True
- False
Questão 20
Questão
A Phagemid is virally inserted DNA in plasmid form
Responda
- True
- False
Questão 21
Questão
Why would a vector be given an M13 origin and inserted with M13?
Responda
-
The M13 phage will treat the plasmid as its own DNA and replicate it for secretion
-
The phage will insert the vector into the chromosome
-
The phage will prevent the bacteria from destroying the plasmid
Questão 22
Questão
Cosmids are plasmids with...?
Responda
-
Cos lambda sites
-
Cos beta sites
-
Cos gamma sites
Questão 23
Questão
Which of the following has the largest limit?
Responda
-
BAC's
-
YAC's
-
MAC's
Questão 24
Questão
Why do you remove a phosphate from the ends of bacterial DNA using a phosphatase?
Image:
Remove_P (image/png)
Responda
-
To prevent it self ligating
-
To prevent it degrading
-
To prevent it getting destroyed
Questão 25
Questão
Why mix enzyme T4 and ATP together with the DNA to be inserted?
Responda
-
Adding AMP to ligated ends gives easily ligable high energy form
-
ATP & T4 remove the phosphates from the ends of the DNA to prevent self annealing
-
T4 can only ligate DNA in the presence of ATP
Questão 26
Questão
A vector must be used because E.Coli cannot take up linear DNA
Responda
- True
- False
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