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457038
Creating an E.Coli Library
Descrição
Year 2 Quiz sobre Creating an E.Coli Library, criado por gina_evans0312 em 29-12-2013.
Sem etiquetas
genetics and genomics
recombinant dna
year 2
Quiz por
gina_evans0312
, atualizado more than 1 year ago
Mais
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Criado por
gina_evans0312
quase 11 anos atrás
478
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Resumo de Recurso
Questão 1
Questão
Electroporations- passing electricity through a mix of DNA and bacteria causes DNA to be taken up
Responda
True
False
Questão 2
Questão
What does N stand for?
Image:
Library_Size (image/png)
Responda
The no of clones needed
P of getting a clone with the desired gene
Genome size
Questão 3
Questão
What are the meanings of i and g?
Image:
Library_Size (image/png)
Responda
i = Library size g= probability of getting desired gene
i = avg insert size g = genome size
i = probability of getting desired gene g = genome size
Questão 4
Questão
The larger the genome, the larger the library required to replicate it
Responda
True
False
Questão 5
Questão
When is cDNA used?
Responda
When an organism has a lot of non-coding DNA
When an organism is very small
When the DNA is degraded
Questão 6
Questão
How is cDNA made?
Responda
Converted from mRNA
Converted from snoRNA
Converted from siRNA
Questão 7
Questão
Less than 1% of the genome is mRNA, but it's exclusively expressible genes
Responda
True
False
Questão 8
Questão
What is required to make the first RNA-DNA hybrid?
Image:
Hybrid (image/png)
Responda
dNTP's
Primer
Reverse Transcriptase
Questão 9
Questão
What are the two methods of degrading the RNA part?
Responda
Alkylisation (RNA is less stable)
RNA digestion through RNAase H
Boiling
Restriction enzymes
Questão 10
Questão
Which method was used to get the following DNA strand?
Image:
Alkali_Degrading (image/png)
Responda
Alkali degrading
Boiling
Shaking
RNAase H
Questão 11
Questão
What is required to get from the former to the latter?
Image:
Alkali_Degrading (image/png)
Responda
dNTP's
DNA Polymerase
Primer
Reverse transcriptase
Questão 12
Questão
What method was used to degrade the RNA into the following?
Image:
RNAase_H (image/png)
Responda
RNAase H
Alkylation
Boiling
Sonication
Questão 13
Questão
What is needed to get from the former to the latter?
Image:
Final_Product (image/png)
Responda
dNTP's
DNA Polymerase H
RNAase
rNTP's
Questão 14
Questão
When creating cDNA, a little information is always lost from the 3' end
Responda
True
False
Questão 15
Questão
How do we prevent this loss of information?
Responda
After degrading the RNA, add a homopolymer (polyC) tail to the 3 end
Using Terminal Transferase
Before degrading the DNA, degrade the Poly A tail
Questão 16
Questão
Then in second strand synthesis add ...
Responda
dNTP's
DNA polymerase
Oligo-dG primer
Questão 17
Questão
Enriching desired DNA means creating/finding more of it
Responda
True
False
Questão 18
Questão
Selective Hybridisation removes cDNA's common to more than one tissue
Responda
True
False
Questão 19
Questão
Tissue Specificity is useful if the gene is only expressed in a certain tissue
Responda
True
False
Questão 20
Questão
What is Normalisation?
Responda
Melt, anneal and isolate ssDNA
Use restriction enzymes to repeatdely cut and ligate DNA
Use DNA helicase to continually replicate DNA
Questão 21
Questão
How does Normalisation work?
Responda
Repetitive DNA binds to other repetitive DNA
Leaving only unique DNA single stranded
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