457038
Descrição
Quiz por gina_evans0312, atualizado more than 1 year ago
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Criado por gina_evans0312
mais de 10 anos atrás
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Questão 1
Questão
Electroporations- passing electricity through a mix of DNA and bacteria causes DNA to be taken up
Responda
- True
- False
Questão 2
Responda
-
The no of clones needed
-
P of getting a clone with the desired gene
-
Genome size
Questão 3
Responda
-
i = Library size g= probability of getting desired gene
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i = avg insert size g = genome size
-
i = probability of getting desired gene g = genome size
Questão 4
Questão
The larger the genome, the larger the library required to replicate it
Responda
- True
- False
Questão 5
Questão
When is cDNA used?
Responda
-
When an organism has a lot of non-coding DNA
-
When an organism is very small
-
When the DNA is degraded
Questão 6
Questão
How is cDNA made?
Responda
-
Converted from mRNA
-
Converted from snoRNA
-
Converted from siRNA
Questão 7
Questão
Less than 1% of the genome is mRNA, but it's exclusively expressible genes
Responda
- True
- False
Questão 8
Responda
-
dNTP's
-
Primer
-
Reverse Transcriptase
Questão 9
Questão
What are the two methods of degrading the RNA part?
Responda
-
Alkylisation (RNA is less stable)
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RNA digestion through RNAase H
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Boiling
-
Restriction enzymes
Questão 10
Responda
-
Alkali degrading
-
Boiling
-
Shaking
-
RNAase H
Questão 11
Responda
-
dNTP's
-
DNA Polymerase
-
Primer
-
Reverse transcriptase
Questão 12
Responda
-
RNAase H
-
Alkylation
-
Boiling
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Sonication
Questão 13
Responda
-
dNTP's
-
DNA Polymerase H
-
RNAase
-
rNTP's
Questão 14
Questão
When creating cDNA, a little information is always lost from the 3' end
Responda
- True
- False
Questão 15
Questão
How do we prevent this loss of information?
Responda
-
After degrading the RNA, add a homopolymer (polyC) tail to the 3 end
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Using Terminal Transferase
-
Before degrading the DNA, degrade the Poly A tail
Questão 16
Questão
Then in second strand synthesis add ...
Responda
-
dNTP's
-
DNA polymerase
-
Oligo-dG primer
Questão 17
Questão
Enriching desired DNA means creating/finding more of it
Responda
- True
- False
Questão 18
Questão
Selective Hybridisation removes cDNA's common to more than one tissue
Responda
- True
- False
Questão 19
Questão
Tissue Specificity is useful if the gene is only expressed in a certain tissue
Responda
- True
- False
Questão 20
Questão
What is Normalisation?
Responda
-
Melt, anneal and isolate ssDNA
-
Use restriction enzymes to repeatdely cut and ligate DNA
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Use DNA helicase to continually replicate DNA
Questão 21
Questão
How does Normalisation work?
Responda
-
Repetitive DNA binds to other repetitive DNA
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Leaving only unique DNA single stranded
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