Creating an E.Coli Library

Descrição

Year 2 Quiz sobre Creating an E.Coli Library, criado por gina_evans0312 em 29-12-2013.
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Quiz por gina_evans0312, atualizado more than 1 year ago
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Criado por gina_evans0312 quase 11 anos atrás
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Resumo de Recurso

Questão 1

Questão
Electroporations- passing electricity through a mix of DNA and bacteria causes DNA to be taken up
Responda
  • True
  • False

Questão 2

Questão
What does N stand for?
Responda
  • The no of clones needed
  • P of getting a clone with the desired gene
  • Genome size

Questão 3

Questão
What are the meanings of i and g?
Responda
  • i = Library size g= probability of getting desired gene
  • i = avg insert size g = genome size
  • i = probability of getting desired gene g = genome size

Questão 4

Questão
The larger the genome, the larger the library required to replicate it
Responda
  • True
  • False

Questão 5

Questão
When is cDNA used?
Responda
  • When an organism has a lot of non-coding DNA
  • When an organism is very small
  • When the DNA is degraded

Questão 6

Questão
How is cDNA made?
Responda
  • Converted from mRNA
  • Converted from snoRNA
  • Converted from siRNA

Questão 7

Questão
Less than 1% of the genome is mRNA, but it's exclusively expressible genes
Responda
  • True
  • False

Questão 8

Questão
What is required to make the first RNA-DNA hybrid?
Responda
  • dNTP's
  • Primer
  • Reverse Transcriptase

Questão 9

Questão
What are the two methods of degrading the RNA part?
Responda
  • Alkylisation (RNA is less stable)
  • RNA digestion through RNAase H
  • Boiling
  • Restriction enzymes

Questão 10

Questão
Which method was used to get the following DNA strand?
Responda
  • Alkali degrading
  • Boiling
  • Shaking
  • RNAase H

Questão 11

Questão
What is required to get from the former to the latter?
Responda
  • dNTP's
  • DNA Polymerase
  • Primer
  • Reverse transcriptase

Questão 12

Questão
What method was used to degrade the RNA into the following?
Responda
  • RNAase H
  • Alkylation
  • Boiling
  • Sonication

Questão 13

Questão
What is needed to get from the former to the latter?
Responda
  • dNTP's
  • DNA Polymerase H
  • RNAase
  • rNTP's

Questão 14

Questão
When creating cDNA, a little information is always lost from the 3' end
Responda
  • True
  • False

Questão 15

Questão
How do we prevent this loss of information?
Responda
  • After degrading the RNA, add a homopolymer (polyC) tail to the 3 end
  • Using Terminal Transferase
  • Before degrading the DNA, degrade the Poly A tail

Questão 16

Questão
Then in second strand synthesis add ...
Responda
  • dNTP's
  • DNA polymerase
  • Oligo-dG primer

Questão 17

Questão
Enriching desired DNA means creating/finding more of it
Responda
  • True
  • False

Questão 18

Questão
Selective Hybridisation removes cDNA's common to more than one tissue
Responda
  • True
  • False

Questão 19

Questão
Tissue Specificity is useful if the gene is only expressed in a certain tissue
Responda
  • True
  • False

Questão 20

Questão
What is Normalisation?
Responda
  • Melt, anneal and isolate ssDNA
  • Use restriction enzymes to repeatdely cut and ligate DNA
  • Use DNA helicase to continually replicate DNA

Questão 21

Questão
How does Normalisation work?
Responda
  • Repetitive DNA binds to other repetitive DNA
  • Leaving only unique DNA single stranded

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