457080
Descrição
Quiz por gina_evans0312, atualizado more than 1 year ago
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Criado por gina_evans0312
quase 11 anos atrás
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Questão 1
Questão
A radioactive probe can take what forms?
Responda
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ssDNA
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RNA
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Denatured dsDNA
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Oligonucleotides
Questão 2
Questão
Probes can be
Responda
-
Radioactive
-
Fluorescent
-
Use Ig's
Questão 3
Questão
Name the two ways probes can be designed
Responda
-
Guesswork
-
Codon Bias
Questão 4
Questão
What is codon bias?
Responda
-
Where cells prefer one codon of an aa to another
-
Where cells will reject certain codons due to what they contain
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Where some cells can only make tRNA's for certain codons
Questão 5
Questão
80% of Phe codons are TTC, 20% are TTT
Responda
- True
- False
Questão 6
Questão
If the protein is an enzyme it can be found by adding the substrate
Responda
- True
- False
Questão 7
Questão
State the possible forms of Ig's for protein separation?
Responda
-
Immobalised on a column
-
Removable from solution
Questão 8
Questão
Probes needn't be 100% accurate to work
Responda
- True
- False
Questão 9
Questão
For a Western Blot, how is the DNA stuck to the membrane?
Responda
-
Denatured DNA sticks to membrane
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Bacteria are lysed to release DNA
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DNA washes off
Questão 10
Questão
Once the DNA has been bound, what happens next?
Responda
-
A probe is added
-
The membrane is run through a gel
-
The membrane is stained
Questão 11
Questão
In an expression vector, where is the new gene added?
Responda
-
The Multiple Cloning Site
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The Promotor Region
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Next to the origin
Questão 12
Responda
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Origin
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Antibiotic Resistance
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Promoter
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Terminator
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Multiple Cloning Site
Questão 13
Questão
More compliated vectors have proteins tagged for purification
Responda
- True
- False
Questão 14
Questão
What is involved in His Chain Purification?
Responda
-
The addition of a His chain to the N terminus
-
The removal of a His chain from the protein
-
The use of a His chain to cut unwatned proteins
Questão 15
Questão
His Purification uses Histadine's affinity for what metal?
Responda
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Ni2+
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Fe2+
-
Na+
Questão 16
Questão
His-tagged proteins will bind to Nickel in a column. Others that don't bind will be washed off.
Responda
- True
- False
Questão 17
Questão
The PET system involves using a promoter for the protein that you can control the expression of
Responda
- True
- False
Questão 18
Questão
What promoter is used in the PET system to control protein expression?
Responda
-
T7
-
T8
-
T9
Questão 19
Questão
In the PET system, what promoter is used to control the T_ expression?
Responda
-
Lac promoter
-
Slo Promoter
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Pap promoter
Questão 20
Questão
What is required for a good shuttle vector?
Responda
-
Replicable in both hosts
-
Selectable in both hosts
-
Expressable in both hosts
Questão 21
Questão
Shuttle vectors are usually plasmids with additional sequences
Responda
- True
- False
Questão 22
Questão
Shuttle vectors for yeast/fungi need
Responda
-
Special origin
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Antibiotic/metabolic marker
Questão 23
Questão
A baclovirus would be used as a vector into what?
Responda
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Insects
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Plants
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Mammals
Questão 24
Questão
Secretion of certain proteins can be caused by what?
Responda
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Disulphide bonds
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Acetylation
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Methylation
Questão 25
Responda
-
One for the yeast to use
-
One for the bacteria to use
Questão 26
Responda
-
URA3- allows growth in the absence of Uracil
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TRA3- allows growth in the absence of thymidine
-
GRA3- allows growth in the absence of guanine
Questão 27
Questão
Like bacteria, yeast cannot take up linear DNA
Responda
- True
- False
Questão 28
Questão
What does a YAC contain that a plasmid does not?
Responda
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Non-useful genes
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Yeast centromere
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Yeast telomeres
Questão 29
Questão
Yeast can allow DNA to be directly inserted into its genome
Responda
- True
- False
Questão 30
Questão
How can yeast accept DNA into its genome?
Responda
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Homologous recombination
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Non-homologous end joining
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Direct injection
Questão 31
Questão
In order to directly insert a gene, you must flank the gene with identical stretches of the part of the genome you want to insert it in
Responda
- True
- False
Questão 32
Questão
Plants would use an aglobacterium T1 plasmid
Responda
- True
- False
Questão 33
Questão
How do you replicate transformed plant cells?
Responda
-
Grown them as plantlets
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Clone them
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Asexually reproduce them
Questão 34
Questão
Mammal shuttle vectors are the same as yeast vectors, but the yeast origin is replaced with what?
Responda
-
An SV40 promoter
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A UG35 promoter
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A FT12 promoter
Questão 35
Questão
Retroviruses can also be used to insert transgenes into mammilian genomes by packing the transgene in a retroviral capsule
Responda
- True
- False
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