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Microbiology Practical F

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Raymond MCC
Elle Ashe
Quiz por Elle Ashe, atualizado more than 1 year ago
Elle Ashe
Criado por Elle Ashe mais de 10 anos atrás
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Resumo de Recurso

Questão 1

Questão
Obligate intracellular parasite
Responda
  • Organisms that can only grow inside another living host.
  • Viruses that infect bacteria.
  • Viral replication cycle that ends in the death of the host cell.

Questão 2

Questão
Bacteriophage
Responda
  • Viruses that infect bacteria.
  • Organisms that can only grow inside another living host cell.
  • Viral replication cycle that ends in death of its host cell.

Questão 3

Questão
Lytic cycle
Responda
  • Viral replication cycle that ends in death of its host cell.
  • Organisms that can only grow inside another living host cell.
  • Viruses that infect bacteria.

Questão 4

Questão
How does a medium used for bacterial growth differ from media used for bacteriophage growth?
Responda
  • Use ordinary media to grow bacteria but, a bacteriophage needs a host cell.
  • Use ordinary media to grow bacteriophages but, bacteria needs a host cell.

Questão 5

Questão
What is the purpose of making serial dilutions of the T-4 phage?
Responda
  • To obtain a countable plate of 30 - 300 plaques.
  • To obtain a countable plate of 30 - 300 colonies.
  • To obtain a countable plate of 30 - 300 individual viruses.

Questão 6

Questão
What is a viral plaque and what does it represent?
Responda
  • An area of clearing on an agar representing a spot where a virus landed, infected a bacteria and lysed it.
  • An area on an agar representing a spot where a bacteria landed, infected a virus and lysed it.
  • An area on an agar representing a spot where a virus landed, infect a yeast and lysed it.

Questão 7

Questão
What purpose does the E. coli serve in this procedure?
Responda
  • It is the host cell for the T4 bacteriophage
  • It is the host cell of the T4 bacteria.
  • It is the host cell of the T4 fungus.

Questão 8

Questão
Explain why Coccidioides immitis is considered to be a dimorphic fungus.
Responda
  • It can grow as a yeast and as a mold.
  • It can grow as a yeast and a virus.
  • It can grow as a mold and a virus.

Questão 9

Questão
What are the growth differences between fungus and bacteria?
Responda
  • Fungi grow faster, at a lower temperature and at a lower pH than bacteria.
  • Fungi grow faster, at a higher temperature and at a higher pH than bacteria.
  • Fungi grow slower, at a lower temperature and at a lower pH than bacteria.
  • Fungi grow slower, at a higher temperature and at a higher pH than bacteria.

Questão 10

Questão
Staphylococcus and Micrococcus are"
Responda
  • Gram (+) cocci in clusters
  • Gram (-) cocci in clusters
  • Staphylococcus is gram (+) in clusters and Micrococcus is gram (-) in pairs
  • Staphylococcus is gram (-) in clusters and Micrococcus is gram (+) in pairs

Questão 11

Questão
Where are Staphylococcus and Micrococcus normally found in the body?
Responda
  • Skin and mucus membranes
  • Intestines
  • Gastrointestinal tract
  • Skin and hair

Questão 12

Questão
Where are Staphylococcus and Micrococcus normally found in the body?
Responda
  • Skin and mucus membranes
  • Intestines
  • Gastrointestinal tract
  • Skin and hair

Questão 13

Questão
People that harbor the pathogen but exhibit no symptoms.
Responda
  • Carrier
  • MRSA
  • Nosocomial
  • Giver

Questão 14

Questão
Methicillin-resistant Staphylococcus aureus
Responda
  • MRSA
  • Carrier
  • Nosocomial
  • Multiple Resistant Streptococcus Aureus

Questão 15

Questão
Hospital acquired
Responda
  • Nosocomial
  • Carrier
  • Giver
  • Catalase

Questão 16

Questão
Which substances that Staphylococcus aureus produce to cause disease
Responda
  • Catalase
  • Coagulase
  • Hyaluronidase
  • Carilase

Questão 17

Questão
When do staphylococcus epidermidis and Micrococcus cause disease?
Responda
  • They are opportunistic pathogens.
  • All the time
  • Never

Questão 18

Questão
Differentiate the colony appearance of Staphylococcus and Streptococcus
Responda
  • Staph are larger, convex, opague and pigmented, Strep are smaller, translucent to slightly opaque.
  • Strep are larger, convex, opague and pigmented, Straph are smaller, translucent to slightly opaque.
  • Staph are smaller, convex, opague and pigmented, Strep are larger, translucent to slightly opaque.

Questão 19

Questão
Which enzyme helps differentiate all staphylococci from streptococci?
Responda
  • Catalase
  • Streptocase
  • Staphoolase

Questão 20

Questão
How did Lancefield divide the beta-hemolytic streptococci into groups?
Responda
  • C polysaccharide extracted from the cell walls
  • Peptidoglycan extracted from the cell walls
  • Lipids extracted from the cell walls

Questão 21

Questão
What is the species name of Lancefield Group A streptococci.
Responda
  • Streptococcus pyogenes
  • Streptococcus aureus
  • Streptococcus epidermidis
  • Streptococcus agalaticae

Questão 22

Questão
What is the species name of Lancefield Group B streptococci?
Responda
  • Streptococcus agalaticae
  • Streptococcus aureus
  • Streptococcus pyogenes
  • Streptococcus cocci

Questão 23

Questão
Where are Enterococcus normally found int he body?
Responda
  • Gastrointestinal tract
  • Skin and mucus
  • Liver
  • Kidneys

Questão 24

Questão
VRE?
Responda
  • Vancomycin resistant enterococcus
  • Vancomycin resistant ebolacoccus
  • Vancomycin resistant enteroboccus

Questão 25

Questão
Viridans streptococci
Responda
  • Alpha hemolytic streptococci other than streptococcus pneumonea
  • Beta hemolytic streptococci other than streptococcus pneumonea
  • Alpha heolytic staphylococci other than staphylococcus pneumonea
  • Beta heolytic staphylococci other than staphylococcus pneumonea

Questão 26

Questão
Group A Strep causes __________________
Responda
  • Strep throat
  • Community-acquired pneumonia
  • Neonatal septicemia
  • Urinary tract infection

Questão 27

Questão
Group B Strep causes _____________
Responda
  • Neonatal septicemia
  • Strep throat
  • Urinary tract infection
  • Community-acquired pneumonia

Questão 28

Questão
Enterococcus causes _________________
Responda
  • Urinary tract infection
  • Dental cavities
  • Strep throat
  • Neonatal speticemia

Questão 29

Questão
Streptococcus pneumoniae causes _________________
Responda
  • Community-acquired pneumonia
  • Neonatal septicemia
  • Urinary tract infection
  • Strep throat

Questão 30

Questão
Viridans streptococci
Responda
  • Dental cavities
  • Strep throat
  • Urinary tract infection
  • Neonatal septicemia

Questão 31

Questão
Why is Coccidioides immitis considered to be a dimorphic fungus?
Responda
  • It can grow as a yeast and as a mold
  • It can grow in pairs.
  • It can grow as coccus or bacillus.

Questão 32

Questão
What are the growth factors between fungus and bacteria?
Responda
  • Fungi grow slower, at a lower temp and lower pH than bacteria
  • Fungi grow faster, at a higher temp and higher pH than bacteria
  • Fungi grow slower, at a higher temp and lower pH than bacteria
  • Fungi grow faster, at a lower temp and higher pH than bacteria

Questão 33

Questão
Explain the difference between vegetative hyphae and aerial hyphae.
Responda
  • Vegetative hyphae grow on or down into the agar surface; aerial hyphae grow above the agar surface.
  • Aerial hyphae grow on or down into the agar surface; vegetative hyphae grow above the agar surface.

Questão 34

Questão
The difference between sporangiospores and conidiospores:
Responda
  • Sporangipospores are inside of a sac and condidiospores are the end of hyphae.
  • Condidiospores are inside of a sac and sporangipospores are the end of hyphae.

Questão 35

Questão
How are molds identified in the laboratory?
Responda
  • Macroscopic appearance
  • Microscopic appearance
  • Hyphal structure

Questão 36

Questão
What are the characteristics of Enterobacteriacea?
Responda
  • All are gram (-) rods
  • All are gram (+) cocci
  • All ferment glucose
  • All reduce nitrate to nitrite
  • They are oxidase (-)
  • They are oxidase (+)
  • They do not ferment glucose

Questão 37

Questão
What are the characteristics of non-fermenters?
Responda
  • All are gram (-)
  • All ferment glucose
  • They do not ferment glucose
  • They are all oxidase (-)
  • Many are oxidase (+)
  • All are gram (+)

Questão 38

Questão
What are the two major groups of gram (-) rods?
Responda
  • Enterobacteriacaea
  • Non-fermenters
  • Staphylococcus
  • Streptococcus

Questão 39

Questão
Which carbohydrate is used to determine whether a gram negative rod is a "Nonfermenter" or "Enterobacteriacae"?
Responda
  • Glucose
  • Lactose

Questão 40

Questão
What carbohydrate(s) are in the Kigler Iron Agar media?
Responda
  • Glucose
  • Lactose
  • Sucrose
  • Galactose

Questão 41

Questão
What carbohydrate(s) are in the MacConkey agar?
Responda
  • Glucose
  • Lactose
  • Sucrose
  • Galactose

Questão 42

Questão
Why is MacConkey agar considered to be a selective and differential media?
Responda
  • It is selective because it contains bile salts and crystal violet which inhibit the growth of gram (+) organisms. It is differential because it contains lactose making it differential for lactose fermentation.
  • It is selective because it contains bile salts and crystal violet which inhibit the growth of gram (-) organisms. It is differential because it contains lactose making it differential for lactose fermentation.
  • It is differential because it contains bile salts and crystal violet which inhibit the growth of gram (+) organisms. It is selective because it contains lactose making it differential for lactose fermentation.
  • It is differential because it contains bile salts and crystal violet which inhibit the growth of gram (-) organisms. It is selective because it contains lactose making it differential for lactose fermentation.

Questão 43

Questão
How many types of organisms will you identify in the mixed unknown exercise?
Responda
  • 1
  • 2
  • 4

Questão 44

Questão
What type of organism grows on MacConkey agar?
Responda
  • Gram (-)
  • Gram (+)

Questão 45

Questão
What ingredient on the MacConkey agar will differentiate the organisms growing on the agar?
Responda
  • Lactose
  • Bile salts and crystal violet
  • Blood

Questão 46

Questão
What type of organism grows on CNA?
Responda
  • Gram (+)
  • Gram (-)

Questão 47

Questão
What ingredient in the CNA media allows differentiation of the organisms growing on the agar?
Responda
  • Bile salts and crystal violet
  • Blood
  • Lactose

Questão 48

Questão
Why is CNA media incubated in a candle jar?
Responda
  • Streptococcus are microaerophiles
  • Streptococcus are anaerobes

Questão 49

Questão
Why is a cotton swab used to obtain the sample from the original broth?
Responda
  • To make sure we get both organisms from the mixed culture
  • To make sure enough sample is collected
  • To make it easier to inoculate the media

Questão 50

Questão
Why is T. soy agar used to grow the "stock cultures"?
Responda
  • It's a neutral media that all bacteria like
  • It is a selective media that only gram (-) bacteria like.
  • It is a selective media that only gram (+) bacteria like.

Questão 51

Questão
Why do you need to develop two flow charts in order to identify your unknown organisms?
Responda
  • You have to develop a dichotomous key for each of your unknown organisms and we have two organisms.
  • You have to develop a dichotomous key for each depending on the outcome of growth on the selective media.

Questão 52

Questão
What is an "enteric" organism?
Responda
  • Part of the normal flora of the intestinal tract
  • Bacteria that live inside another organism.

Questão 53

Questão
How are culture media designed to detect if a bacteria can produce a specific enzyme?
Responda
  • Substrates are incorporated into the culture media that the enzymes act upon and that along with indicator system lets you see the products.
  • Enzymes are incorporated into the media and an indicator systems lets you see if they match the organism.

Questão 54

Questão
What is the purpose of the Durham tube in the carbohydrate fermentation tubes?
Responda
  • Collects gas
  • Releases gas
  • Keeps tube from exploding

Questão 55

Questão
Name the specific tests that are part of the IMViC test
Responda
  • Indole
  • Methyl red
  • Voges-Proskauer
  • Citrate
  • Urease

Questão 56

Questão
Name 3 Enterobacteriaceae normally found in the gastrointestinal tract.
Responda
  • E. coli
  • Enterobacter aerogenes
  • Proteus vulgaris
  • Salmonella
  • Klebsiella pneumoniae
  • Shigella

Questão 57

Questão
Name two Enterobacteriaceae that are major pathogens and state the disease they cause.
Responda
  • Salmonella - Gastroenteritis, typhoid fever
  • Shigella - Bacterial dysentery
  • E. coli - Gastroenteritis, typhoid fever
  • Proteus vulgaris - Bacterial dysentery

Questão 58

Questão
Which commercial test systems are used to identify gram (-) rods in clinical labs?
Responda
  • Entero tube
  • API
  • 20E
  • Micro ID
  • Micro tube
  • APE

Questão 59

Questão
Why do you use the streak-stab method for blood agar inoculations?
Responda
  • So you can see the hemolysis better
  • To see if the bacteria like to grow in or on the agar

Questão 60

Questão
Where do you grow streptococcus and why?
Responda
  • In a candle jar because it's a microaerophile
  • In the air because it's an aerobe

Questão 61

Questão
Which bacteria are alpha and optochin sensitive?
Responda
  • Streptococcus pneumoniae
  • Streptococcus mitis
  • Streptococcus pyogenes
  • Group C Streptococcus
  • Enterococcis faecalis

Questão 62

Questão
Which bacteria are and optochin resistant?
Responda
  • Streptococcus mitis
  • Streptococcus pneumoniae
  • Streptococcus pyogenes
  • Group C Streptococcus
  • Enterococcus faecalis

Questão 63

Questão
Which bacteria are beta and bacitracin sensitive?
Responda
  • Streptococcus pyogenes
  • Streptococcus pneumoniae
  • Streptococcus mitis
  • Group C Streptococcus
  • Enterococcus faecalis

Questão 64

Questão
Which bacteria are beta and bacitracin resistant?
Responda
  • Group C Streptococcus
  • Streptococcus mitis
  • Streptococcus pneumoniae
  • Enterococcus faecalis
  • Streptococcus pyogenes

Questão 65

Questão
How do you tell the difference between the alpha hemolytic Streptococcus and and Enterococcus faecalis?
Responda
  • The alpha Streptococcus are bile esculin (-) and the Enterococcus faecalis is bile esculin (+)
  • The alpha Streptococcus are bile esculin (+) and the Enterococcus faecalis is bile esculin (-)

Questão 66

Questão
How does the catalase test differentiate between Staphylococcus and Streptococcus?
Responda
  • Staphylococcus is catalase (+) and Streptococcus is catalase (-)
  • Staphylococcus is catalase (-) and Streptococcus is catalase (+)

Questão 67

Questão
What kind of hemolysis is shown on this plate?
Image:
3_Alpha_hemolysis_Strep_mitis_CNA (image/jpg)
Responda
  • Alpha hemolyisis
  • Beta Hemolysis
  • Gamma Hemolysis

Questão 68

Questão
What is the mold pictured?
Image:
3a_Aspergillus_Septate_hyphae (image/jpg)
Responda
  • Aspergillis
  • Penicillium
  • Rhizopus

Questão 69

Questão
What is the mold pictured?
Image:
3a_Aspergillus__10x__Lactophenol_cotton_blue (image/jpg)
Responda
  • Aspergillus
  • Penicillium
  • Rhizopus

Questão 70

Questão
What is the mold pictured?
Image:
3a_Aspergillus__10x__Lactophenol_cotton_blue (image/jpg)
Responda
  • Aspergillus
  • Penicillium
  • Rhizopus

Questão 71

Questão
What is the mold pictured?
Image:
3a_Aspergillus__400x (image/jpg)
Responda
  • Aspergillus
  • Penicillium
  • Rhuzopus

Questão 72

Questão
What is the mold pictured?
Image:
3a_Aspergillus__400x (image/jpg)
Responda
  • Aspergillus
  • Penicillium
  • Rhizopus

Questão 73

Questão
What is the mold pictured?
Image:
3a_Aspergillus_niger (image/jpg)
Responda
  • Aspergillus
  • Penicillium
  • Rhizopus

Questão 74

Questão
What is the mold pictured?
Image:
3a_Penicillium_Culture (image/jpg)
Responda
  • Aspergillus
  • Penicillium
  • Rhizopus

Questão 75

Questão
What mold is pictured?
Image:
3a_Fungi_Apergillus_slide_culture (image/jpg)
Responda
  • Aspergillus
  • Penicillium
  • Rhizopus

Questão 76

Questão
What mold is pictured?
Image:
3a_Micro_Fungi_Micro_Fungi_032 (image/jpg)
Responda
  • Aspergillus
  • Penicillium
  • Rhizopus

Questão 77

Questão
What mold is pictured?
Image:
3a_Penicillium_Slide_Culture (image/jpg)
Responda
  • Aspergillus
  • Penicillium
  • Rhizopus

Questão 78

Questão
What mold is pictured?
Image:
3a_Penicillium__Lactophenol_cotton_blue__40x (image/jpg)
Responda
  • Aspergillus
  • Penicillium
  • Rhizopus

Questão 79

Questão
What mold is pictured?
Image:
3a_Rhizopu_on_a_plate (image/jpg)
Responda
  • Aspergillus
  • Penicillium
  • Rhizopus

Questão 80

Questão
What mold is pictured?
Image:
3a_Rhizopus_zygospore__40x (image/jpg)
Responda
  • Aspergillus
  • Penicillium
  • Rhizopus

Questão 81

Questão
What mold is pictured?
Image:
3a_Rhizopus__40x (image/jpg)
Responda
  • Aspergillus
  • Penicillium
  • Rhizopus

Questão 82

Questão
What mold is pictured?
Image:
3a_rhizopus (image/jpg)
Responda
  • Aspergillus
  • Penicillium
  • Rhizopus

Questão 83

Questão
Is this result positive or negative for beta lactamase?
Image:
3aBeta_lactamase_test_positive_A (image/jpg)
Responda
  • Positive
  • Negative

Questão 84

Questão
Is this result positive or negative for beta lactamase?
Image:
3a_Beta_lactamase_test_negative_A (image/jpg)
Responda
  • Positive
  • Negative

Questão 85

Questão
What are the results of the bile esculin test pictured?
Image:
3_Bile (image/jpg)
Responda
  • A = negative, B = positive
  • A = positive, B = negative

Questão 86

Questão
What are the results of the carbohydrate fermentation pictured?
Image:
3_Carbohydrate_fermentation.JPG (image/JPG)
Responda
  • A = negative, B = positive, C = positive + gas
  • A = positive, B = negative, C = negative +gas

Questão 87

Questão
What are the results of the citrate test pictured?
Image:
3_Citrate.JPG (image/JPG)
Responda
  • A = negative, B = positive
  • A = positive, B = negative

Questão 88

Questão
What are the results of the indole test pictured?
Image:
3_INDOLE.JPG (image/JPG)
Responda
  • A = negative, B = positive
  • A = positive, B = negative

Questão 89

Questão
What is the result of the lactose fermentation pictured?
Image:
3_macconkey (image/jpg)
Responda
  • A = negative, B = positive, C = positive
  • A = negative, B = negative, C = positive
  • A = positive, B = negative, C = negative
  • A = positive, B = positive, C = negative

Questão 90

Questão
What are the results of the motility test pictured?
Image:
3_motilitylabeled (image/jpg)
Responda
  • A = non-motile, B = motile, C = motile
  • A = non-motile, B = non-motile, C = motile
  • A = motile, B =motile, C = non-motile
  • A = motile, B = non-motile, C = non-motile

Questão 91

Questão
What are the results of the sensitivity test pictured?
Image:
3_opto_srpn-srvi (image/jpg)
Responda
  • A = sensitive, B = resistant
  • A = resistant, B = sensitive

Questão 92

Questão
How do you calculate the titer of phage in the original sample given the number of plaques and the dilution of the plate?
Responda
  • # of plaques x dilution x 10^-1 = _________ PFU/mL
  • # of plaques x dilution x 10^1= __________ PFU/mL

Questão 93

Questão
What kind of hemolysis is pictured?
Image:
3_Gamma_hemolysis_M_luteus_Blood_Agar (image/jpg)
Responda
  • Gamma
  • Alpha
  • Beta

Questão 94

Questão
Which bacteria is cogulase positive and manitol slat agar positive?
Responda
  • Staphylococcus aureus
  • Staphylococcus epiderminis
  • Micrococcus roseus
  • Micrococcus luteus

Questão 95

Questão
Which bacteria is coagulase negative and manitol salt agar negative?
Responda
  • Staphylococcus epidermidis
  • Stapylococcus aureus
  • Micrococcus roseus
  • Micrococcus luteus

Questão 96

Questão
What bacteria is positive for nitrate reduction and rose for pigment production?
Responda
  • Micrococcus roseus
  • Micrococcus luteus
  • Staphylococcus aureus
  • Staphylococcus epidermidis

Questão 97

Questão
What bacteria is negative for nitrate reduction and yellow for pigment production?
Responda
  • Micrococcus luteus
  • Micrococcus roseus
  • Staphylococcus aureus
  • Staphylococcus epidermidis

Questão 98

Questão
How is Enterococcus faecalis differentiated from other alpha hemolytic streptococcus?
Responda
  • It is positive for bile esculin
  • It is negative for bile esculin

Questão 99

Questão
Which Streptococcus bacteria is optochin resistant?
Responda
  • Streptococcus mitis
  • Streptococcus pneumoniae
  • Streptococcus pyogenes
  • Group C streptococcus

Questão 100

Questão
Which streptococcus bacteria is optochin sensitive?
Responda
  • Streptococcus pneumoniae
  • Streptococcus mitis
  • Streptococcus pyogenes
  • Group C Streptococcus

Questão 101

Questão
Which bacteria is bacitration resistant?
Responda
  • Group C Streptococcus
  • Streptococcus pyogenes
  • Streptococcus pneumoniae
  • Streptococcus mitis

Questão 102

Questão
Which bacteria is bacitracin sensitive?
Responda
  • Streptococcus pyogenes
  • Streptococcus mitis
  • Streptococcus pneumoniae
  • Group C Streptococcus

Questão 103

Questão
Which bacteria are alpha hemolytic?
Responda
  • Streptococcus mitis
  • Streptococcus pneumoniae
  • Streptococcus pyogenes
  • Group C Streptococcus

Questão 104

Questão
Which bacteria are beta hemolytic?
Responda
  • Streptococcus mitis
  • Streptococcus pneumoniae
  • Streptococcus pyogenes
  • Group C Streptococcus

Questão 105

Questão
What hemolysis is pictured?
Image:
Staph_hemolysis_Blood_Agar (image/jpg)
Responda
  • A= alpha, B = beta, C = gamma
  • A = beta, B = alpha, C = gamma
  • A = gamma, B = alpha, C = beta

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