Questão 1
Questão
Obligate intracellular parasite
Responda
-
Organisms that can only grow inside another living host.
-
Viruses that infect bacteria.
-
Viral replication cycle that ends in the death of the host cell.
Questão 2
Responda
-
Viruses that infect bacteria.
-
Organisms that can only grow inside another living host cell.
-
Viral replication cycle that ends in death of its host cell.
Questão 3
Responda
-
Viral replication cycle that ends in death of its host cell.
-
Organisms that can only grow inside another living host cell.
-
Viruses that infect bacteria.
Questão 4
Questão
How does a medium used for bacterial growth differ from media used for bacteriophage growth?
Responda
-
Use ordinary media to grow bacteria but, a bacteriophage needs a host cell.
-
Use ordinary media to grow bacteriophages but, bacteria needs a host cell.
Questão 5
Questão
What is the purpose of making serial dilutions of the T-4 phage?
Responda
-
To obtain a countable plate of 30 - 300 plaques.
-
To obtain a countable plate of 30 - 300 colonies.
-
To obtain a countable plate of 30 - 300 individual viruses.
Questão 6
Questão
What is a viral plaque and what does it represent?
Responda
-
An area of clearing on an agar representing a spot where a virus landed, infected a bacteria and lysed it.
-
An area on an agar representing a spot where a bacteria landed, infected a virus and lysed it.
-
An area on an agar representing a spot where a virus landed, infect a yeast and lysed it.
Questão 7
Questão
What purpose does the E. coli serve in this procedure?
Responda
-
It is the host cell for the T4 bacteriophage
-
It is the host cell of the T4 bacteria.
-
It is the host cell of the T4 fungus.
Questão 8
Questão
Explain why Coccidioides immitis is considered to be a dimorphic fungus.
Responda
-
It can grow as a yeast and as a mold.
-
It can grow as a yeast and a virus.
-
It can grow as a mold and a virus.
Questão 9
Questão
What are the growth differences between fungus and bacteria?
Responda
-
Fungi grow faster, at a lower temperature and at a lower pH than bacteria.
-
Fungi grow faster, at a higher temperature and at a higher pH than bacteria.
-
Fungi grow slower, at a lower temperature and at a lower pH than bacteria.
-
Fungi grow slower, at a higher temperature and at a higher pH than bacteria.
Questão 10
Questão
Staphylococcus and Micrococcus are"
Responda
-
Gram (+) cocci in clusters
-
Gram (-) cocci in clusters
-
Staphylococcus is gram (+) in clusters and Micrococcus is gram (-) in pairs
-
Staphylococcus is gram (-) in clusters and Micrococcus is gram (+) in pairs
Questão 11
Questão
Where are Staphylococcus and Micrococcus normally found in the body?
Responda
-
Skin and mucus membranes
-
Intestines
-
Gastrointestinal tract
-
Skin and hair
Questão 12
Questão
Where are Staphylococcus and Micrococcus normally found in the body?
Responda
-
Skin and mucus membranes
-
Intestines
-
Gastrointestinal tract
-
Skin and hair
Questão 13
Questão
People that harbor the pathogen but exhibit no symptoms.
Responda
-
Carrier
-
MRSA
-
Nosocomial
-
Giver
Questão 14
Questão
Methicillin-resistant Staphylococcus aureus
Questão 15
Questão
Hospital acquired
Responda
-
Nosocomial
-
Carrier
-
Giver
-
Catalase
Questão 16
Questão
Which substances that Staphylococcus aureus produce to cause disease
Responda
-
Catalase
-
Coagulase
-
Hyaluronidase
-
Carilase
Questão 17
Questão
When do staphylococcus epidermidis and Micrococcus cause disease?
Questão 18
Questão
Differentiate the colony appearance of Staphylococcus and Streptococcus
Responda
-
Staph are larger, convex, opague and pigmented, Strep are smaller, translucent to slightly opaque.
-
Strep are larger, convex, opague and pigmented, Straph are smaller, translucent to slightly opaque.
-
Staph are smaller, convex, opague and pigmented, Strep are larger, translucent to slightly opaque.
Questão 19
Questão
Which enzyme helps differentiate all staphylococci from streptococci?
Responda
-
Catalase
-
Streptocase
-
Staphoolase
Questão 20
Questão
How did Lancefield divide the beta-hemolytic streptococci into groups?
Responda
-
C polysaccharide extracted from the cell walls
-
Peptidoglycan extracted from the cell walls
-
Lipids extracted from the cell walls
Questão 21
Questão
What is the species name of Lancefield Group A streptococci.
Questão 22
Questão
What is the species name of Lancefield Group B streptococci?
Responda
-
Streptococcus agalaticae
-
Streptococcus aureus
-
Streptococcus pyogenes
-
Streptococcus cocci
Questão 23
Questão
Where are Enterococcus normally found int he body?
Responda
-
Gastrointestinal tract
-
Skin and mucus
-
Liver
-
Kidneys
Questão 24
Responda
-
Vancomycin resistant enterococcus
-
Vancomycin resistant ebolacoccus
-
Vancomycin resistant enteroboccus
Questão 25
Questão
Viridans streptococci
Responda
-
Alpha hemolytic streptococci other than streptococcus pneumonea
-
Beta hemolytic streptococci other than streptococcus pneumonea
-
Alpha heolytic staphylococci other than staphylococcus pneumonea
-
Beta heolytic staphylococci other than staphylococcus pneumonea
Questão 26
Questão
Group A Strep causes __________________
Questão 27
Questão
Group B Strep causes _____________
Questão 28
Questão
Enterococcus causes _________________
Responda
-
Urinary tract infection
-
Dental cavities
-
Strep throat
-
Neonatal speticemia
Questão 29
Questão
Streptococcus pneumoniae causes _________________
Questão 30
Questão
Viridans streptococci
Responda
-
Dental cavities
-
Strep throat
-
Urinary tract infection
-
Neonatal septicemia
Questão 31
Questão
Why is Coccidioides immitis considered to be a dimorphic fungus?
Questão 32
Questão
What are the growth factors between fungus and bacteria?
Responda
-
Fungi grow slower, at a lower temp and lower pH than bacteria
-
Fungi grow faster, at a higher temp and higher pH than bacteria
-
Fungi grow slower, at a higher temp and lower pH than bacteria
-
Fungi grow faster, at a lower temp and higher pH than bacteria
Questão 33
Questão
Explain the difference between vegetative hyphae and aerial hyphae.
Questão 34
Questão
The difference between sporangiospores and conidiospores:
Questão 35
Questão
How are molds identified in the laboratory?
Responda
-
Macroscopic appearance
-
Microscopic appearance
-
Hyphal structure
Questão 36
Questão
What are the characteristics of Enterobacteriacea?
Questão 37
Questão
What are the characteristics of non-fermenters?
Questão 38
Questão
What are the two major groups of gram (-) rods?
Responda
-
Enterobacteriacaea
-
Non-fermenters
-
Staphylococcus
-
Streptococcus
Questão 39
Questão
Which carbohydrate is used to determine whether a gram negative rod is a "Nonfermenter" or "Enterobacteriacae"?
Questão 40
Questão
What carbohydrate(s) are in the Kigler Iron Agar media?
Responda
-
Glucose
-
Lactose
-
Sucrose
-
Galactose
Questão 41
Questão
What carbohydrate(s) are in the MacConkey agar?
Responda
-
Glucose
-
Lactose
-
Sucrose
-
Galactose
Questão 42
Questão
Why is MacConkey agar considered to be a selective and differential media?
Responda
-
It is selective because it contains bile salts and crystal violet which inhibit the growth of gram (+) organisms. It is differential because it contains lactose making it differential for lactose fermentation.
-
It is selective because it contains bile salts and crystal violet which inhibit the growth of gram (-) organisms. It is differential because it contains lactose making it differential for lactose fermentation.
-
It is differential because it contains bile salts and crystal violet which inhibit the growth of gram (+) organisms. It is selective because it contains lactose making it differential for lactose fermentation.
-
It is differential because it contains bile salts and crystal violet which inhibit the growth of gram (-) organisms. It is selective because it contains lactose making it differential for lactose fermentation.
Questão 43
Questão
How many types of organisms will you identify in the mixed unknown exercise?
Questão 44
Questão
What type of organism grows on MacConkey agar?
Questão 45
Questão
What ingredient on the MacConkey agar will differentiate the organisms growing on the agar?
Questão 46
Questão
What type of organism grows on CNA?
Questão 47
Questão
What ingredient in the CNA media allows differentiation of the organisms growing on the agar?
Questão 48
Questão
Why is CNA media incubated in a candle jar?
Questão 49
Questão
Why is a cotton swab used to obtain the sample from the original broth?
Responda
-
To make sure we get both organisms from the mixed culture
-
To make sure enough sample is collected
-
To make it easier to inoculate the media
Questão 50
Questão
Why is T. soy agar used to grow the "stock cultures"?
Responda
-
It's a neutral media that all bacteria like
-
It is a selective media that only gram (-) bacteria like.
-
It is a selective media that only gram (+) bacteria like.
Questão 51
Questão
Why do you need to develop two flow charts in order to identify your unknown organisms?
Responda
-
You have to develop a dichotomous key for each of your unknown organisms and we have two organisms.
-
You have to develop a dichotomous key for each depending on the outcome of growth on the selective media.
Questão 52
Questão
What is an "enteric" organism?
Questão 53
Questão
How are culture media designed to detect if a bacteria can produce a specific enzyme?
Responda
-
Substrates are incorporated into the culture media that the enzymes act upon and that along with indicator system lets you see the products.
-
Enzymes are incorporated into the media and an indicator systems lets you see if they match the organism.
Questão 54
Questão
What is the purpose of the Durham tube in the carbohydrate fermentation tubes?
Questão 55
Questão
Name the specific tests that are part of the IMViC test
Responda
-
Indole
-
Methyl red
-
Voges-Proskauer
-
Citrate
-
Urease
Questão 56
Questão
Name 3 Enterobacteriaceae normally found in the gastrointestinal tract.
Responda
-
E. coli
-
Enterobacter aerogenes
-
Proteus vulgaris
-
Salmonella
-
Klebsiella pneumoniae
-
Shigella
Questão 57
Questão
Name two Enterobacteriaceae that are major pathogens and state the disease they cause.
Responda
-
Salmonella - Gastroenteritis, typhoid fever
-
Shigella - Bacterial dysentery
-
E. coli - Gastroenteritis, typhoid fever
-
Proteus vulgaris - Bacterial dysentery
Questão 58
Questão
Which commercial test systems are used to identify gram (-) rods in clinical labs?
Responda
-
Entero tube
-
API
-
20E
-
Micro ID
-
Micro tube
-
APE
Questão 59
Questão
Why do you use the streak-stab method for blood agar inoculations?
Questão 60
Questão
Where do you grow streptococcus and why?
Questão 61
Questão
Which bacteria are alpha and optochin sensitive?
Responda
-
Streptococcus pneumoniae
-
Streptococcus mitis
-
Streptococcus pyogenes
-
Group C Streptococcus
-
Enterococcis faecalis
Questão 62
Questão
Which bacteria are and optochin resistant?
Responda
-
Streptococcus mitis
-
Streptococcus pneumoniae
-
Streptococcus pyogenes
-
Group C Streptococcus
-
Enterococcus faecalis
Questão 63
Questão
Which bacteria are beta and bacitracin sensitive?
Responda
-
Streptococcus pyogenes
-
Streptococcus pneumoniae
-
Streptococcus mitis
-
Group C Streptococcus
-
Enterococcus faecalis
Questão 64
Questão
Which bacteria are beta and bacitracin resistant?
Responda
-
Group C Streptococcus
-
Streptococcus mitis
-
Streptococcus pneumoniae
-
Enterococcus faecalis
-
Streptococcus pyogenes
Questão 65
Questão
How do you tell the difference between the alpha hemolytic Streptococcus and and Enterococcus faecalis?
Questão 66
Questão
How does the catalase test differentiate between Staphylococcus and Streptococcus?
Questão 67
Questão
What kind of hemolysis is shown on this plate?
Responda
-
Alpha hemolyisis
-
Beta Hemolysis
-
Gamma Hemolysis
Questão 68
Questão
What is the mold pictured?
Responda
-
Aspergillis
-
Penicillium
-
Rhizopus
Questão 69
Questão
What is the mold pictured?
Responda
-
Aspergillus
-
Penicillium
-
Rhizopus
Questão 70
Questão
What is the mold pictured?
Responda
-
Aspergillus
-
Penicillium
-
Rhizopus
Questão 71
Questão
What is the mold pictured?
Responda
-
Aspergillus
-
Penicillium
-
Rhuzopus
Questão 72
Questão
What is the mold pictured?
Responda
-
Aspergillus
-
Penicillium
-
Rhizopus
Questão 73
Questão
What is the mold pictured?
Responda
-
Aspergillus
-
Penicillium
-
Rhizopus
Questão 74
Questão
What is the mold pictured?
Responda
-
Aspergillus
-
Penicillium
-
Rhizopus
Questão 75
Questão
What mold is pictured?
Responda
-
Aspergillus
-
Penicillium
-
Rhizopus
Questão 76
Questão
What mold is pictured?
Responda
-
Aspergillus
-
Penicillium
-
Rhizopus
Questão 77
Questão
What mold is pictured?
Responda
-
Aspergillus
-
Penicillium
-
Rhizopus
Questão 78
Questão
What mold is pictured?
Responda
-
Aspergillus
-
Penicillium
-
Rhizopus
Questão 79
Questão
What mold is pictured?
Responda
-
Aspergillus
-
Penicillium
-
Rhizopus
Questão 80
Questão
What mold is pictured?
Responda
-
Aspergillus
-
Penicillium
-
Rhizopus
Questão 81
Questão
What mold is pictured?
Responda
-
Aspergillus
-
Penicillium
-
Rhizopus
Questão 82
Questão
What mold is pictured?
Responda
-
Aspergillus
-
Penicillium
-
Rhizopus
Questão 83
Questão
Is this result positive or negative for beta lactamase?
Questão 84
Questão
Is this result positive or negative for beta lactamase?
Questão 85
Questão
What are the results of the bile esculin test pictured?
Responda
-
A = negative, B = positive
-
A = positive, B = negative
Questão 86
Questão
What are the results of the carbohydrate fermentation pictured?
Responda
-
A = negative, B = positive, C = positive + gas
-
A = positive, B = negative, C = negative +gas
Questão 87
Questão
What are the results of the citrate test pictured?
Responda
-
A = negative, B = positive
-
A = positive, B = negative
Questão 88
Questão
What are the results of the indole test pictured?
Responda
-
A = negative, B = positive
-
A = positive, B = negative
Questão 89
Questão
What is the result of the lactose fermentation pictured?
Responda
-
A = negative, B = positive, C = positive
-
A = negative, B = negative, C = positive
-
A = positive, B = negative, C = negative
-
A = positive, B = positive, C = negative
Questão 90
Questão
What are the results of the motility test pictured?
Responda
-
A = non-motile, B = motile, C = motile
-
A = non-motile, B = non-motile, C = motile
-
A = motile, B =motile, C = non-motile
-
A = motile, B = non-motile, C = non-motile
Questão 91
Questão
What are the results of the sensitivity test pictured?
Responda
-
A = sensitive, B = resistant
-
A = resistant, B = sensitive
Questão 92
Questão
How do you calculate the titer of phage in the original sample given the number of plaques and the dilution of the plate?
Questão 93
Questão
What kind of hemolysis is pictured?
Questão 94
Questão
Which bacteria is cogulase positive and manitol slat agar positive?
Questão 95
Questão
Which bacteria is coagulase negative and manitol salt agar negative?
Questão 96
Questão
What bacteria is positive for nitrate reduction and rose for pigment production?
Questão 97
Questão
What bacteria is negative for nitrate reduction and yellow for pigment production?
Questão 98
Questão
How is Enterococcus faecalis differentiated from other alpha hemolytic streptococcus?
Questão 99
Questão
Which Streptococcus bacteria is optochin resistant?
Responda
-
Streptococcus mitis
-
Streptococcus pneumoniae
-
Streptococcus pyogenes
-
Group C streptococcus
Questão 100
Questão
Which streptococcus bacteria is optochin sensitive?
Responda
-
Streptococcus pneumoniae
-
Streptococcus mitis
-
Streptococcus pyogenes
-
Group C Streptococcus
Questão 101
Questão
Which bacteria is bacitration resistant?
Responda
-
Group C Streptococcus
-
Streptococcus pyogenes
-
Streptococcus pneumoniae
-
Streptococcus mitis
Questão 102
Questão
Which bacteria is bacitracin sensitive?
Responda
-
Streptococcus pyogenes
-
Streptococcus mitis
-
Streptococcus pneumoniae
-
Group C Streptococcus
Questão 103
Questão
Which bacteria are alpha hemolytic?
Responda
-
Streptococcus mitis
-
Streptococcus pneumoniae
-
Streptococcus pyogenes
-
Group C Streptococcus
Questão 104
Questão
Which bacteria are beta hemolytic?
Responda
-
Streptococcus mitis
-
Streptococcus pneumoniae
-
Streptococcus pyogenes
-
Group C Streptococcus
Questão 105
Questão
What hemolysis is pictured?
Responda
-
A= alpha, B = beta, C = gamma
-
A = beta, B = alpha, C = gamma
-
A = gamma, B = alpha, C = beta