Questão 1
Questão
Name the components of nutrient media
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Glucose Broth
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Meat-Peptone Broth
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Meat-Peptone agar
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Blood Agar
Questão 2
Questão
Most of the bacterial colonies are visualized after:
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20 minutes
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2 hours
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1-2 days
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1 week
Questão 3
Questão
According to their cell wall structures bacteria divide into
Questão 4
Questão
Extra chromosomal genetic elements in bacteria are
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Mesosomes
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Phagosomes
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Transposons
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Plasmids
Questão 5
Questão
How many phases have growth curve of bacteria?
Ans: [blank_start]4[blank_end]
Name them:
a/ [blank_start]Lag[blank_end] Phase
b/ [blank_start]Log[blank_end] Phase
c/ [blank_start]Stationary[blank_end] Phase
d/ [blank_start]Decline[blank_end] Phase
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4
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Lag
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Log
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Stationary
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Decline
Questão 6
Questão
Sex-pili of bacteria helps for their
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Movement
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Conjugation
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Nutrition
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Protection
Questão 7
Questão
Name the three shapes of bacteria:
a/ [blank_start]Round[blank_end] ([blank_start]Cocci[blank_end])
b/ [blank_start]Rods[blank_end]
c/ [blank_start]Spiral[blank_end] ([blank_start]Spirilli[blank_end])
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Round
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Cocci
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Rods
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Spiral
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Spirilli
Questão 8
Questão
What is the most resistant to an unappropiate conditions bacterial form?
Questão 9
Questão
Which of the following bacterial structures prevents phagocytosis?
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Cytoplasm
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Peptidoglycan
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Capsule
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Nuclues
Questão 10
Questão
Are flagellas an obligatory structure of bacterium?
Questão 11
Questão
What chemical is responsible for the acid-fast property of mycobacteria?
Ans: [blank_start]Mycolic acids[blank_end]
Questão 12
Questão
Name two bacteria that have capsules:
a/ [blank_start]E. coli[blank_end]
b/ [blank_start]Salmonella[blank_end]
Questão 13
Questão
In microbiology what is the most commonly used objective
Ans: [blank_start]Oil[blank_end]
Explain:
This is because [blank_start]oil[blank_end] unlike other liquids like water has very [blank_start]little light refraction[blank_end]
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Oil
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oil
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little light refraction
Questão 14
Questão
What is the name of staining method for Corynebacterium diphtheriae?
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Gram
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Moller
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Zhiehl-Neelsen
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Neisser
Questão 15
Questão
Gram (+) bacteria are stained in [blank_start]blue[blank_end] colour and Gram (-) in [blank_start]red[blank_end] colour
Questão 16
Questão
The main taxonomic category for bacteria is
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Genus
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Species
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Family
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Ordo
Questão 17
Questão
According Gram, the Gram (-) bacteria are stained in [blank_start]red[blank_end] colour
Questão 18
Questão
The unit for bacterial size is:
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milimeter
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micrometer
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nanometer
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angstrom
Questão 19
Questão
Vibrions are curved bacteria with
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4-6 curves
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many curves
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one curve
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two curves
Questão 20
Questão
Ziehl-Neelsen is a staining procedure in:
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encapsulated bacteria
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spore-forming bacteria
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acid-fast bacteria
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motile bacteria
Questão 21
Questão
Bacterial cytoplasm contains:
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capsule
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ribosomes
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nucleotide
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peptidoglycan
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pili
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inclusions
Questão 22
Questão
Name the purposes of simple staining:
a/ Determine cell [blank_start]shape[blank_end]
b/ Determine cell [blank_start]size[blank_end]
c/ Determine cell [blank_start]arrangement[blank_end]
Questão 23
Questão
The main staining procedure that detect morphology of bacteria is:
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Loeffler
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Neisser
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Gram
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Pfeiffer
Questão 24
Questão
The name Baccilus antracis defines certain bacterial
Questão 25
Questão
Is it true: Bacterial spores are formed in unfavorable conditions and are means of multiplying
Questão 26
Questão
Staining according Victoria blau is a [blank_start]simple[blank_end] method
Questão 27
Questão
Which of the following organelles are essential for bacteria?
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cytoplasm membrane
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pili
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capsule
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flagella
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nucleoid
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ribosomes
Questão 28
Questão
What are the curved bacteria?
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staphylococci
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helicobacter
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vibrions
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bacilli
Questão 29
Questão
Diplococci are [blank_start]round[blank_end] shaped bacteria that are arranged in [blank_start]pairs[blank_end]
Questão 30
Questão
Staphylococci are round shaped bacteria arranged in [blank_start]clusters[blank_end]
Questão 31
Questão
The volutine bodies are inclusions in the cytoplasm of:
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menigococci
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diptheria bacteria
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genococci
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pneumococci
Questão 32
Questão
Where is lipopolysaccaride situated?
Questão 33
Questão
Name at least two rod-shaped bacteria
a/ [blank_start]Bacillus anthracis[blank_end]
b/ [blank_start]Clostridium tetani[blank_end]
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Bacillus anthracis
-
Clostridium tetani
Questão 34
Questão
How many spores form one bacteria?
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one
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two
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three
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more than one
Questão 35
Questão
Loefler technique stain bacteria in [blank_start]blue[blank_end] colour
Questão 36
Questão
The causative agent of tuberculosis is stained in red color, using [blank_start]Ziehl[blank_end] [blank_start]Neelsen[blank_end] (name the staining method)
Questão 37
Questão
What are the two purposes of heat fixation
a/ [blank_start]kill[blank_end] organisms
b/ allows stain to be [blank_start]absorbed[blank_end]
Questão 38
Questão
Each bacteria has two names and they define its
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family and genus
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family and kingdom
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genus and species
Questão 39
Questão 40
Questão
Which of the following is NOT found in the cell wall of gram (-) bacteria?
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Lipid A
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Teichoic acids
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O-antigen
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peptidoglycan
Questão 41
Questão
Name the different types of bacteria pili and what are their function
a/ [blank_start]common[blank_end] and its function is [blank_start]adhesion[blank_end]
b/ [blank_start]sex[blank_end] and its function is [blank_start]conjugation[blank_end]
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common
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adhesion
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sex
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conjugation
Questão 42
Questão
Loeffler stain requires
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Methylene blue
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Ziehl fuschin
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Gentian violet
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Iodine solution
Questão 43
Questão
Which of the following methods of staining are complex
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Pfeiffer
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Neisser
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Klett
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Loeffler
Questão 44
Questão
Bacterium Diphteri and its structure : [blank_start]Volutine granule[blank_end]
Questão 45
Questão
The volutine granules of Bacterium Diphteri are visualized by : [blank_start]Neisser[blank_end] staining
Questão 46
Questão
The following structures are localized in the cytoplasm:
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Spores
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Nucleoids
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Flagellum
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Capsules
Questão 47
Questão
Based on their cellular structure bacteria are divided into:
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Cocci and rods
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Gram + and Gram –
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Spares and spiral
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Aerobes and anaerobe
Questão 48
Questão
Peptides Glycan polymer are found only in :
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Bacterial cell
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Animal cell
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In viruses
Questão 49
Questão
Chemical structure of the capsule is composed of :
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Complex polysaccharides
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Vitamins
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Polypeptides
Questão 50
Questão
Sporulation appears to be :
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An initiation of reproduction of bacterial cell
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A reaction of conditions that are unfavorable for normal growth
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A status to resist desiccation and other harmful conditions
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a & b
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b & c
Questão 51
Questão
The complete killing or removal of all microorganisms including bacteria spores is called :
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Sterilization
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Disinfection
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Antisepsis
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Sepsis
Questão 52
Questão
What is the color of the simple method of Pfeiffer staining : [blank_start]red[blank_end]
Questão 53
Questão
Pfeiffer technical staining makes use of the following :
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Methylene blue
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Carbon Fuchsine
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Neutral red
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Gentian violet
Questão 54
Questão
The cell wall of gram (+) bacteria is composed mainly :
Questão 55
Questão
Which of the following bacteria have peptidoglycan layer:
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Gram (+) cocci
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Gram (-) bacteria
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Both of them
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None above
Questão 56
Questão
Cellular DNA is concentrated in (what place) :
Questão 57
Questão
The spores stain by Moller are :
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Blue color
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Red color
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Black color
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Yellow color
Questão 58
Questão
Which structure is NOT protecting bacteria from phagocytosis from leukocytes:
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Spores
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Capsule
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Flagella
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Ribosome
Questão 59
Questão
Name two simple (basic) stains
a/ [blank_start]Loeffler[blank_end]
b/ [blank_start]Pfeiffer[blank_end]
Questão 60
Questão
The concentration of ethyl alcohol as an antiseptic for disinfection is :
Questão 61
Questão
Bacterial nucleoid consists of :
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No chromosomes
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Only one chromosome
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Several chromosomes
Questão 62
Questão
What is the name of staining procedure for bacterial capsule:
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Gram stain
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Loeffler stain
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Klett stain
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Moller stain
Questão 63
Questão
Lipopolysaccharide can be found in large amount :
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Gram (+)
-
Gram (-)
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Both of them
-
None of above
Questão 64
Questão
Complex method for bacterial staining are :
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Loeffler
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Pfeiffer
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Gram
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Ziehl-Neelsen
Questão 65
Questão
Bacterial antibiotic resistance is frequently covered by :
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Tempered bacteriophage
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R-plasmids
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Lipid bacteriophage
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Intron
Questão 66
Questão
What constitutes a bacterial culture :
a/ [blank_start]Solute[blank_end]
b/ dry [blank_start]agar[blank_end]
c/ [blank_start]broth[blank_end]
Questão 67
Questão
Which one is a liquid medium for bacterial culture:
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Blood agar
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Glucose broth
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Both of them
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None above
Questão 68
Questão
Anaerobes are bacteria that can tolerate high oxygen concentration
Questão 69
Questão
What bacterial physiologist studies :
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Morphology of bacteria
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Bacterial structure
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Bacterial growth
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Bacterial nutrient
Questão 70
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Sterilizing agent
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Antiseptic
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Antibiotic
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None is true
Questão 71
Questão
Which of the following is most important structure related to microbial attachment to cells :
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Flagellum
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Plasmid
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Peptidoglycan
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Ordinary Pilli
Questão 72
Questão
Which statement about genetic transfer among bacteria requires cell to cell contact:
Questão 73
Questão
Which of the following related to endotoxin is not true:
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Endotoxins are secreted from living cells
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Endotoxins are part of cellular gram (-) bacteria
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They are secreted only when the cell is disrupted
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They can be linked to meningococcemia
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They are produced by gram(-) microorganisms
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They cause fever
Questão 74
Questão
What cause (hint) to be pink :
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Carbon Fuchsin
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Iodine solution
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Crystal violet
-
Neisser A
Questão 75
Questão
Gram (-) bacteria differ from Gram (+) ones because the posses :
Questão 76
Questão
The auto-sterilisation requires the following :
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1:30 hours at 160 C
-
134 C for 10-15 minutes
Questão 77
Questão
The chlorine is :
Questão 78
Questão
Concerning bacterial cell division everything is true except that :
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All bacteria double their numbers every 10 hours
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Bacterial generation times are influenced by environmental conditions
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Two bacterial cells following division are genetically identical and they are once
Questão 79
Questão
How does high temperature cause cell death?
Questão 80
Questão
How are the actions of phenols and alcohols similar :
Questão 81
Questão
What is pasteurization :
Questão 82
Questão
Bacterial flagella are :
Questão 83
Questão
Name the complex nutrient media :
Responda
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Glucose broth
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Mid peptone broth
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Mid peptone agar
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Blood agar
Questão 84
Questão
Extra chromosomal genetic elements in bacteria are :
Questão 85
Questão
What part of the bacterial cell is most involved with Gram staining and why?
Ans: [blank_start]Cell wall[blank_end]
Explanation: Gram [blank_start]positive[blank_end] bacteria have a [blank_start]thick[blank_end] peptidoglycan cell [blank_start]wall[blank_end] that holds the stain [blank_start]better[blank_end] than the [blank_start]thin[blank_end] peptidoglycan gram [blank_start]negative[blank_end] cell [blank_start]wall[blank_end]
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positive
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thick
-
wall
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better
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thin
-
negative
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wall
-
Cell wall
Questão 86
Questão
What is the purpose of heat during the acid-fast staining procedure?
The heat [blank_start]drives[blank_end] the stain [blank_start]into[blank_end] the cells
Questão 87
Questão
Name two curved bacteria:
a. [blank_start]Vibrio Cholerae[blank_end]
b. [blank_start]Helicobacteria[blank_end]
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Vibrio Cholerae
-
Helicobacteria
Questão 88
Questão
Name the reagent used and state the purpose of each of the following
a/ mordant: Reagent- [blank_start]Iodine[blank_end] solution, Function: form a [blank_start]complex[blank_end] between the crystal violet and [blank_start]iodine[blank_end]
b/ primary stain: Reagent- [blank_start]Gentian Violet[blank_end], Function: increases the [blank_start]contrast[blank_end] of both gram-negative and gram-positive bacteria
c/ decolorizer: Reagent- [blank_start]Alcohol[blank_end], Function: [blank_start]dehydrates[blank_end] the peptidoglycan layer
d/ counterstain: Reagent- [blank_start]carbol fuschin[blank_end], Function: stains solution light [blank_start]red[blank_end] allowing Gram [blank_start]negative[blank_end] to be seen
Responda
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Alcohol
-
Iodine
-
complex
-
iodine
-
Gentian Violet
-
contrast
-
dehydrates
-
carbol fuschin
-
red
-
negative
Questão 89
Questão
Round bacteria in chains are named [blank_start]streptococci[blank_end]
Questão 90
Questão
What suggestions about endotoxins are true?
a/ they are typical for [blank_start]Gram (-)[blank_end]
b/ they act [blank_start]non-specific[blank_end]
c/ their chemical structure is [blank_start]lipopolysaccarides[blank_end]
d/ they are heat [blank_start]stable[blank_end]
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Gram (-)
-
Gram (+)
-
non-specific
-
specific
-
lipopolysaccarides
-
proteins
-
stable
-
labile