BIOL2022 L22 nMDS and hypothesis testing

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Module 3, Lecture 4 Headings: • Hypothesis testing • nMDS • ANOSIM • SIMPER
Michael Jardine
Quiz by Michael Jardine, updated more than 1 year ago
Michael Jardine
Created by Michael Jardine over 6 years ago
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Resource summary

Question 1

Question
Label the steps appropriately (please).
Answer
  • (dis)similarity matrix
  • nMDS
  • ANOSIM
  • SIMPER
  • ANOVA
  • PERMANOVA

Question 2

Question
Species diversity indexes: Different diversity indices emphasise different things. Two examples are the following. Which emphasises what?
Answer
  • Emphasises common species
  • Emphasises rare species
  • Emphasises non-zero correlations
  • Emphasises zero-correlations
  • Emphasises similarities
  • Emphasises dissimilarities

Question 3

Question
In either the Simpson’s index or the Shannon-Weiner index, despite their differences, they both have high index values if:
Answer
  • Evenness is high
  • Evenness is low
  • There are few species
  • There are many species
  • None of the above

Question 4

Question
In a(n) [blank_start]____________[blank_end], a [blank_start]________[blank_end] stress value means the model is good.
Answer
  • Low
  • High
  • nMDS
  • ANOSIM
  • PERMANOVA
  • SIMPER
  • Bray-Curtis dissimilarity matrix

Question 5

Question
In a Bray-Curtis dissimilarity matrix, a low number means:
Answer
  • High dissimilarity
  • Low dissimilarity
  • High similarity
  • Low similarity

Question 6

Question
ANOSIM is ANalysis Of SIMilarities. Retains philosophy underpinning [blank_start]________[blank_end], driven by [blank_start]________[blank_end]. Hands [blank_start]________[blank_end] designs best. [blank_start]____________[blank_end] permutation test combined with randomisation approach to generate significance levels (Monte Carlo tests) Null – no difference in assemblage composition [blank_start]________[blank_end] sites.
Answer
  • nMDS
  • ANOVA
  • PERMANOVA
  • SIMPER
  • Information theory
  • Hypothesis testing
  • Simple
  • Complex
  • Non-parametric
  • Parametric
  • Metric
  • Non-metric
  • Within
  • Between

Question 7

Question
Steps for ANOSIM: 1. Test statistic – reflects difference among sites to differences within sites. Based on rank similarities in the underlying triangular matrix, generates [blank_start]____________[blank_end]. 2. Recompute [above] under permutations of sample labels. If null is true there should be little change to [above] if labels arbitrarily assigned. 3. Significance value – refer the observed value of [above] to permutation distribution. If it is unlikely (<5%) to fall in this distribution, reject null H
Answer
  • Global R
  • Stress value
  • P value
  • Pearson's correlation

Question 8

Question
Which statistical test works with any distance measure that is appropriate to the data, and uses permutations to make it distribution free?
Answer
  • PERMANOVA
  • ANOSIM
  • ANOVA
  • SIMPER

Question 9

Question
Which statistical test calculates % contribution of each species to the dissimilarities between all pairs of sampling units in different groups and % contribution to similarities between all pairs within groups?
Answer
  • SIMPER
  • PERMANOVA
  • ANOVA
  • ANOVASIM

Question 10

Question
Which one is capable of working with more complex designs?
Answer
  • PERMANOVA
  • ANOSIM
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