To ensure that AHG tests with negative results are not the false negatives
To ensure that washing removed all unbound antibody
To ensure that AHG was not omitted or inactivated
All of the above
Question 2
Question
What is a positive DAT?
Answer
In vitro sensitization of RBC with antigen
In vitro sensitization of RBC with antibody
In vivo sensitization of RBC with antibody
In vivo sensitization of RBC with antigen
Question 3
Question
A positive autocontrol in antibody detection procedures is usually indicative of:
Answer
Inadequate washing
Positive DAT
Positive IAT
two of the above
Question 4
Question
Why is it important to match the lot number on the panel cells?
Answer
The ABO group will change from lot to lot
Pattern of reactions will change from lot to lot
It is a requirement of the FDA
All of the above
Question 5
Question
In interpreting an antibody screen, which of the following questions might be asked to decipher the class of antibody?
Answer
Is the autologous control positive or negative?
Is hemolysis present?
In what phase did the reaction occur?
Is rouleaux present?
Question 6
Question
What is an elution?
Answer
a technique used to dissociate IgM antibodies from sensitized RBCs
a technique used to dissociate IgG antibodies from sensitized RBCs
a technique used to reduce the zeta potential enhancing antigen binding
none of the above
Question 7
Question
What is tested in an antibody screen?
Answer
Patient red cells are tested against group O reagent screening cells
Patient serum is tested against group AB reagent screening cells
Patient serum is tested against group O reagent screening cells
Patient serum is tested against group A reagent screening cells
Question 8
Question
What might a positive antibody screen and a negative autocontrol indicate?
Answer
An alloantibody is coating donor cells after a transfusion
An autoantibody has been detected
An alloantibody has been detected
Drug-induced antibody reacting with patient cells.
Question 9
Question
When might you suspect multiple antibodies in a patient's serum?
Answer
Pattern of reactivity not fitting a single antibody
Variation in phase of reactivity
Variation in antibody reactivity strength
All of the above
Question 10
Question
One drop of Coombs control cells was added to a negative antibody screen. No agglutination was observed after centrifugation. What course of action is taken?
Answer
Report negative result
Report inconclusive result
Repeat the test
Add one more drop of check cells and recentrifuge
Question 11
Question
What is the shipping temp requirement for plasma?
Answer
1 to 6 degree F
1 to 6 degree C
-18 or higher degree F
-18 or lower degree C
Question 12
Question
Which of the following may not be used as a patient identifier?
Answer
Patient's full name
Patients date of birth
Patients medical record number
Patients room number
Question 13
Question
Which of the following is not an enhancement media?
Answer
Albumin
Low Ionic Strength Solution (LISS)
Normal Saline
Polyethylene glycol
Question 14
Question
Additive solutions are approved for storage of red blood cells for how many days?
Answer
21
42
35
7
Question 15
Question
What is the current storage time and storage temp for platelet concentrates and apheresis platelet components?
Answer
5 days at 1 to 6 degrees C
5 days at 24 to 27 degrees C
5 days at 20 to 24 degrees C
7 days at 22 to 24 degrees C
Question 16
Question
Whole blood and RBC units are stored at what temperature?