Copy and Edit

MCB Midterm Summary

Description

Quiz on MCB Midterm Summary, created by Chau Tran on 03/12/2019.
Chau Tran
Quiz by Chau Tran, updated more than 1 year ago
Chau Tran
Created by Chau Tran almost 5 years ago
1
0
0

Resource summary

Question 1

Question
A/an _____________ vector quantifies the expression of a gene to measure the activity of regulatory elements.
Answer
  • Reporter
  • Expression
  • Cloning

Question 2

Question
An expression vector is used to _________.
Answer
  • Study the effect of overexpression of a gene on cell functions
  • Measure activities of regulatory sequences
  • Quantify the expression of a gene downstream of the regulatory sequence of interest
  • Amplify and isolate the product of a gene

Question 3

Question
Which of the following is NOT an essential component of a plasmid?
Answer
  • Ori
  • Antibiotic resistance gene
  • Multiple cloning site
  • lac operon

Question 4

Question
What would not be found in a eukaryotic expression vector?
Answer
  • Bacterial Ori
  • Ampicillin selectable marker
  • Eukaryotic promoter
  • MCS
  • PolyA signal
  • Neomycin - eukaryotic selectable marker
  • Transfection factor
  • luciferase gene

Question 5

Question
The expression of the _______ gene is quantified to study the activity of a regulatory sequence. In which type of vector would you find this gene?
Answer
  • luciferase - expression vector
  • luciferase - reporter vector
  • lacZ - expression vector
  • lacZ - reporter vector

Question 6

Question
Which is NOT involved in the construction of cDNA libraries?
Answer
  • reverse transcriptase
  • oligo dT primer
  • ribonuclease H
  • DNA polymerase
  • DNA ligase

Question 7

Question
What is the role of ribonuclease H in the synthesis of cDNA?
Answer
  • Degrade the mRNA template
  • Synthesize the mRNA template
  • LIgate the 2 strands of newly-synthesized DNA
  • Synthesize the first strand of cDNA from mRNA template

Question 8

Question
Which type of vector is pBluescript?
Answer
  • Expression because it tests for the expression of lacZ gene
  • Cloning because it is used to isolate DNA sequences with a built-in mechanism to select for recombinant plasmids
  • Reporter because it quantifies lacZ expression

Question 9

Question
Which outcome indicates that a colony on the pBluescript agar contains the recombinant plasmid?
Answer
  • Colony is blue
  • Colony is white
  • There is a clearing zone around the colony

Question 10

Question
Which is NOT true about enhancers sequences?
Answer
  • Always located upstream of a gene
  • Function in both 5'-3' and 3'-5' directions
  • Influence expression of genes that are physically distant from the enhancer
  • Function is not affected if location is moved

Question 11

Question
Which of the following are DNA binding motifs?
Answer
  • Helix-turn-helix
  • Zinc finger
  • Leucine zipper
  • Helix-loop-helix

Question 12

Question
_______ hormones bind hormone response elements (HRE) in the nucleus to upregulate target genes.
Answer
  • Steroid
  • Peptide

Question 13

Question
Epigenetics (changes in chromatin structure) is heritable and irreversible.
Answer
  • True
  • False

Question 14

Question
Position-effect variegation is the change in gene expression caused by the translocation of genes between heterochromatin and euchromatin regions.
Answer
  • True
  • False

Question 15

Question
Which does NOT result from histone acetyltransferases (HATs) activity?
Answer
  • Loosening of DNA packaging
  • Decreased gene expression
  • Easier transcriptional factor binding
  • More open chromatin

Question 16

Question
Which would lead to a decrease in gene expression?
Answer
  • HAT
  • HDAC
  • DNA methyltransferases
  • SWI/SNF

Question 17

Question
What is the difference in dosage compensation of Drosophila and C. elegans?
Answer
  • Drosophila: hyperactivation of male X; C. elegans: hypoactivations of both female X'es
  • Drosophila: hypoactivations of both female X'es; C. elegans: hyperactivation of male X
  • Drosophila: inactivation of a female X; C. elegans: hyperactivation of male X
  • Drosophila: hyperactivation of male X; C. elegans: inactivation of a female X

Question 18

Question
Imprinted gene is the inheritance of parental acetylation pattern.
Answer
  • True
  • False

Question 19

Question
Which does NOT result in increased mRNA stability?
Answer
  • elF4E
  • PABPI
  • Deadenylase
  • Long poly-A tail

Question 20

Question
______ removes the hairpin loop of the Pre-miRNA to form ds siRNA.
Answer
  • Drosha
  • Dicer
  • Exp5
  • Ago2

Question 21

Question
G1/S checkpoint is controlled by [blank_start]Rb[blank_end]. DNA damage checkpoint is controlled by [blank_start]p53[blank_end].
Answer
  • Rb
  • p53
  • p21
  • Ras

Question 22

Question
Which is NOT correct regarding cell cycle control?
Answer
  • Phosphorylation of Rb leads to growth factor independent G1/S progression.
  • A loss-of-function mutation in the p53 gene leads to a rapid accumulation of pro-cancer mutations.
  • Cyclin D is an inhibitor of Rb, which in turns is an inhibitor of E2F.
  • p53 upregulates BAX which activates the extrinsic apoptotic pathway.

Question 23

Question
The p53 gene is mutated in >50% cancers.
Answer
  • True
  • False

Question 24

Question
Which gene is the most closely-associated with chronic myelogenous leukemia?
Answer
  • bcr-abl
  • c-myc
  • Ras
  • HER2

Question 25

Question
In Burkitt's lymphoma, ______ gene is translocated to a region controlled by a strong enhancer.
Answer
  • c-myc
  • bcr-abl
  • HER2
  • Ras

Question 26

Question
Which does NOT result in growth factor independent G1/S transition?
Answer
  • Hyperphosphorylated Rb
  • Loss-of-function mutation of Rb
  • Mutation in E2F which prevents Rb binding
  • Low levels of cyclin D leading up to DNA duplication

Question 27

Question
Which of the following cellular properties is the LEAST likely to cause cancer?
Answer
  • Mutations in signalling proteins and receptors
  • Insensitive to anti-growth signals
  • Overexpression of pro-apoptotic proteins
  • Overexpression of telomerase
  • Upregulation of VEGF
  • Ability to metastasize and invade other tissues

Question 28

Question
[blank_start]Ras[blank_end] hyperactivation results from a point mutation to Valine and GTP-independent activity. [blank_start]bcr-abl[blank_end] fusion protein is closely associated with chronic myelogenous leukaemia. [blank_start]HER2[blank_end] is an epidermal growth factor receptor, whose oncogenic properties arises from gene amplification. In Burkitt's lymphoma, [blank_start]c-myc[blank_end] gene is translocated to a region controlled by a strong enhancer. It is also amplified in childhood neuroblastomas.
Answer
  • Ras
  • bcr-abl
  • HER2
  • c-myc
Show full summary Hide full summary

Want to create your own Quizzes for free with GoConqr? Learn more.

Similar

Atoms and Reactions
siobhan.quirk
Silas Marner notes
mehxinee
adjectives and adverbs
kiejrys
IMAGS Employment Examination for Applicants
mike_101290
MCAT Physics - Dynamics
Jen Molte
HSC Chemistry Multiple Choice Quiz
megclark
All Edexcel GCSE PE key terms
Millie Berrett
Physics Unit 2 - Force, Acceleration And Terminal Velocity
Ryan Storey
chemistry: c2
kristy baker
Medical Terminology
khachoe_pema
Power and Conflict Ozymandias
Sophia Hinchliffe
Browse Library