Exam 2

Description

this is for MLT diagnostic microbiology
J P
Quiz by J P, updated more than 1 year ago
J P
Created by J P about 2 years ago
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Resource summary

Question 1

Question
Air flow in a microbiology laboratory should be:
Answer
  • from lower to higher risk areas
  • minimized as much as possible
  • optimized to prevent the settling of dangerous aerosols
  • well-filtered by recirculating it through numerous filters before recycling

Question 2

Question
Procedures such as grinding, mincing, vortexing, and preparing smears:
Answer
  • are hazardous and should be performed while wearing gloves and gown protection.
  • create aerosol droplets and should be performed only in an approved biosafety cabinet.
  • should be avoided with specimens known to contain infectious agents
  • produce a spill hazard and should be performed over an absorptive mat.

Question 3

Question
Class I biological safety cabinets:
Answer
  • sterilize the air as it passes over the material in the cabinet
  • protect the environment from contamination by biological agents by filtering the air as it is exhausted from the cabinet.
  • are completely enclosed and have gloves that enable the worker to manipulate the material in the cabinet.
  • are often called laminar flow hoods.

Question 4

Question
Optimal protection of specimen, personnel, and environment is accomplished with which class of biological safety cabinet?
Answer
  • Class I
  • Class II
  • Class III
  • Class IV

Question 5

Question
The method of choice for sterilizing items such as antibiotic solutions, chemicals, radioisotopes, and vaccines that are heat-sensitive is:
Answer
  • dry, low temperature heat.
  • moist, low temperature heat.
  • filtration
  • chemical

Question 6

Question
To be sterilized in an autoclave, infectious medical waste should be:
Answer
  • packed tightly to avoid dangerous pressure pockets.
  • processed for 15 to 20 minutes at 121 C and 30 pounds per square inch (psi) pressure.
  • processed for 30 to 60 minutes at 132C and 15 psi pressure
  • processed for at least 1 hour at 121 C and 15 psi pressure.

Question 7

Question
The autoclave sterilizes material by:
Answer
  • applying dry heat at high temperatures and pressure.
  • infusing gaseous chemicals that kill organisms.
  • applying moist heat at high temperatures and increased pressure.
  • cleaving protein bonds found in most biological agent cell walls.
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