Disaggregation of Explant

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• DISAGGREGATION  OF  EXPLANTS

• Primary explant technique

• Mechanical diasaggregation

• Dissection of explant

• Finely chopped

• Washed by setting

• Transfer in last wash

• REmove BSS

• Add thin flim of medium

• Explant out growth

• Incubate 2 to 3 weeks

• TISSUE SAMPLE

• CELL+DEBRIS

• MORE CELLS+DEBRIS

• VIABEL CELLS+DEBRIS

• DILUTE THE CELLS

• INCUBATE

• Enzymatic disaggregation

• Biopsy in medium+antibiotic

• Dissection in BSS+antibiotic

• Chopped tissue

• Complete medium+ collagenase

• Dissagregated tissue+ tissue residues ​​

• Dispersed cells+residual culture of epithelial cells​​​​​​

• Dispersed cell suspension contains enriched fibrobiasts

• Enriched epithelial fraction

• Primary cell cul​​​​​​ture