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A microbe growing in a functioning refrigerator is probably a:
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Psychrophile
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Mesophile
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Thermophile
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Hyperthermophile
Frage 2
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A microbe isolated from deep in the ocean could best be characterized as a:
Frage 3
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Addition of blood to a culture medium only allows the haemolytic bacteria that grow on the plate to be picked out. This is an example of a:
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Selective medium
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Differential medium
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Complex medium
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Synthetic medium
Frage 4
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All organisms require the following macronutrients:
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Potassium, magnesium, zinc
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Phosphorus, cobalt, manganese
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Calcium, potassium, zinc
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Phosphorus, potassium, magnesium
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Almost all medically important microbes are:
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Chemolithoautotrophs
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Photoorganoheterotrophs
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Photolithoautotrophs
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Chemoorganoheterotrophs
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An extremophilic microbe is one which:
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Can survive dry conditions
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Can grow only when there is a mixture of stress parameters (e.g. high temperatures and low pH)
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Can grow optimally under extreme conditions
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Becomes dormant under extreme conditions
Frage 7
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Biochemical synthesis of new cell material is called:
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Metabolism.
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Anabolism.
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Catabolism.
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Synthatabolism.
Frage 8
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Carl Woese of the University of Illinois and his collaborators identified two major branches of prokaryotic evolution. What was the basis for dividing prokaryotes into two domains?
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Microscopic examination of the staining characteristics of the cell wall
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Metabolic characteristics such as the production of methane gas
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Ecological characteristics such as the ability to survive extreme temperatures
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Molecular characteristics such as ribosomal RNA sequences
Frage 9
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Carrier mediated transport is necessary when:
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Diffusion will not allow adequate amounts of a substance to enter the cell
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Movement into the cell is against a concentration gradient
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The level of nutrients in nature is very low
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All of the above
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Catalase is indispensable to:
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Obligate anaerobes
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Aerotolerant anaerobes
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Aerobes
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All of the above
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Chemical energy is released when organic or inorganic compounds are:
Frage 12
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Denitrification is a process by which:
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Nitrogen gas is fixed by microorganisms
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Nitrates are reduced to nitrogen gas
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Nitrates are reduced to ammonia
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Nitrogen is assimilated into cellular material
Frage 13
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Incorporation of atmospheric N2 to NH4+ occurs via the process of:
Frage 14
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MacConkey agar selects for:
Frage 15
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Modes of obtaining nutrition, used by at least some bacteria, include all of the following EXCEPT:
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Chemolithoautrophy
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Photolithoautrophy
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Chemoheteroautrophy
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Chemoorganoheterotrophy
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Photoautotophs use:
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Light as an energy source and CO2 as a carbon source
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Light as an energy and methane as a carbon source
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CO2 as both an energy source and a carbon source
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All of the above
Frage 17
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Act as a solvent for iron and transport it into the cells
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Are a key component of cytochromes
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Help maintain osmotic balance in seawater microbes
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Develop when errors in membrane synthesis occur
Frage 18
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The cardinal temperatures are the:
Frage 19
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The CORRECT way to write this microbe’s name is
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bacillus subtilis (italics)
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Bacillus subtilis
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Bacillus Subtilis (italics)
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Bacillus subtilis (italics)
Frage 20
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The signature sequence is used extensively in the:
Frage 21
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The size (as determined by the sedimentation factor) of the most useful RNA molecule for prokaryotic evolutionary studies is:
Frage 22
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Which of the following is a macronutrient?
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Manganese
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Magnesium
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Zinc
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Cobalt
Frage 23
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Which of the following is both a selective and a differential medium?
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Nutrient broth.
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MacConkey agar.
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Blood agar.
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Tryptic soya broth.
Frage 24
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Which of the following forms of oxygen are generally toxic to living organisms?
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Superoxide anion
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Hydrogen peroxide
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Hydroxyl radical
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All of the above
Frage 25
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Which of these environmental factors influence microbial growth?
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pH
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Water potential
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Oxygen
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All of the above
Frage 26
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Which prokaryotes should be expected to be most strongly resistant to plasmolysis in hypertonic environments?
Frage 27
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A Wild-type bacterial strain is:
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A strain with a limited number of mutations
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An arbitrarily chosen strain
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A strain without plasmids
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A strain which is sensitive to antibiotics
Frage 28
Antworten
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Are stretches of double stranded RNA
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Have introns
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Are often organised into operons
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Are always present in two copies
Frage 29
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Bacterial Genomics is:
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A study of the entire bacterial genome
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Sequencing of the bacterial chromosome
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A study of essential genes and genetic elements
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Sequencing of DNA samples obtained from the environment
Frage 30
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Genetic recombination is a:
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Change of DNA structure caused by radiation
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Amplification of DNA molecules followed by cell division
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Physical exchange between DNA molecules
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Process resulting in protein synthesis
Frage 31
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Chemical modifications of DNA or RNA molecules
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Any changes of bacterial genome as compared with a wild type
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Events resulting from genetic exchange between bacterial plasmids
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Sequencing errors
Frage 32
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Post-genomic techniques include:
Frage 33
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The bacterial genome is:
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Mostly diploid but includes several haploid regions
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A single circular DNA molecule
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Mostly haploid
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A double stranded RNA molecule
Frage 34
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Which one of the following sentences correctly describes the science of Metagenomics?
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Metagenomics is a genome level study of the whole microbial community
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Metagenomics is a genomics of industrially and medically important bacteria
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Metagenomics is a study of gene expression
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Metagenomics is a comparative analysis of the sequenced microbial genomes
Frage 35
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With regards to bacterial plasmids which statement is the correct one?
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A plasmid is a regulatory gene
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A plasmid is a short RNA molecule
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A plasmid is an extra chromosomal DNA molecule
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A plasmid is a non coding region found in some bacterial genomes
Frage 36
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A culture of E. coli is diluted by a factor of 10-6 and, after plating out 100 μl on an agar plate, 63 colonies grew after incubation at 37oC overnight. The original culture therefore contained how many viable E coli cells per ml?
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6.3 x 10(7)
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6.3 x 10(8)
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6.3 x 10(6)
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6.3 x 10(5)
Frage 37
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Bacterial flagella bring about cell motion by which mechanism?
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Wave-like movement of the flagellum
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Rotation of the cell
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Rotation of the flagellum in one direction only
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Rotation of the flagellum that is reversible
Frage 38
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Koch’s postulates most CORRECTLY relate to which one of the following statements?
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The isolation of a bacterium in culture from an infection alone indicates that it caused the disease
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Isolation and cultivation of a microorganism from a diseased animal, then inoculation into another healthy animal to cause the same disease, followed by re-isolation of the same microorganism, indicates that it is the cause of the disease
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The observation of virus particles in tissue from a human with disease indicates that it has caused the disease
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Inoculation of a bacterium into an animal and observation of disease alone indicates that it is the cause of the disease observed
Frage 39
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Pasteur is attributed with which one of the following discoveries?
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Was the first to see microorganisms under a microscope
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First to discover and use agar
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That there could be life without air
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The first chemical antibiotics
Frage 40
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The length of a typical E. coli cell is approximately?
Frage 41
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With regard to the differences between bacterial and eukaryotic cells which statement is the correct one?
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Bacterial cells do not contain mitochondria
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Bacterial cells are usually larger than eukaryote cells
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Bacteria do not contain a chromosome
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Bacterial cells are not sensitive to β-lactam antibiotics
Frage 42
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With respect to differences between members of the archaeal and bacterial domains/kingdoms, which is the CORRECT statement?
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Both have peptidoglycan in their cell walls
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Only some Archaea are capable of methanogenesis
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Archaea are sensitive to β-lactam antibiotics
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Bacteria have similar RNA polymerase to that of Archaea
Frage 43
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Which statement is correct with respect to the E.coli chromosome?
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It is negatively supercoiled
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It is positively supercoiled
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There are typically several origins of replication
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It consists of over 50 million base pairs
Frage 44
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Which statement is correct with respect to Lipopolysaccharide (LPS)?
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LPS is found in Gram positive cells
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LPS is found in Gram negative cells in the cytoplasmic membrane
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LPS is found in the outer-membrane of Gram negative cells
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LPS is associated with all bacterial cells
Frage 45
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Which statement is correct with respect to peptidoglycan?
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There are multiple molecules of peptidoglycan in bacterial cell walls
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Only Gram positive cells possess peptidoglycan
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There is only a single molecule of peptidoglycan in a bacterial cell wall
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Peptidoglycan is only found in Gram negative cell walls
Frage 46
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Anthrophilic dermatophytes (fungi causing cutaneous infections of humans) are primarily associated with:
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Soil
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Animals
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Plants
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Humans
Frage 47
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Asexual reproduction in the Mastigomycotina (e.g. Phytophthora spp.) results in the formation of:
Antworten
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Sporangia
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Oospores
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Zygospores
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Conidia
Frage 48
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Black sigatoka is a:
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Fungal root disease of bananas
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Fungal foliar disease of bananas
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Fungal fruit disease of apples
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Fungal foliar disease of potatoes
Frage 49
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Fungal growth and cell division is NOT normally accompanied by:
Frage 50
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Fungi primarily contribute to soil and plant health by:
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Degrading sand particles to release Si
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Facilitating desiccation of water-logged soils
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Degrading organic matter and releasing nutrients
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Preventing bacterial growth
Frage 51
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Fungi tend to live at interfaces. Which of these statements is untrue:
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This can be where nutrients are most concentrated
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Solid surfaces may provide anchorage
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Other microbes, that leak useful metabolites, may inhabit such niches
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There is typically less water on physical surfaces and fungi avoid water
Frage 52
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In the Earth’s biosphere most fungal biomass and diversity is found:
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In the oceans
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In soils
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Under glaciers
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In the atmosphere
Frage 53
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One factor that is NOT used to prevent or reduce fungal spoilage of processed foods is:
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Addition of benzene
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Low water-activity
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Refrigeration
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Heat-sterilization
Frage 54
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Organisms belonging to the genus Phytophthora are:
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True fungi
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Fungus-like organisms
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Slime moulds
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Rusts
Frage 55
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Phytophthora infestans is a fungus-like organism which:
Frage 56
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Phytophthora ramorum is the causal pathogen of:
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Potato blight
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Sudden Oak Death
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Coffee rust
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Ash dieback
Frage 57
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‘Necrosis’ is a symptom of infection of a plant with a pathogenic organism. Necrosis is:
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Yellowing of infected or surrounding tissue
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Uncontrolled cell division resulting in the formation of a gall
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Death of infected or surrounding cells
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Premature leaf fall
Frage 58
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Saprophytic activity is essential for the health of the biosphere because:
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It facilitates nutrient cycling and waste degradation
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It kills host organisms and thereby averts overpopulation
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It facilitates species evolution via endosymbiosis
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It involves mineralization of inorganic rocks in the lithosphere
Frage 59
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Secondary symptoms of infections of plants by pathogens:
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Occur at some distance from the point of infection
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Normally occur close to the site of active infection
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Are an ideal place to isolate the causal organism
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Never occur at the same time as primary symptoms
Frage 60
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Systems biology approaches to the study of fungal cells do NOT necessarily involve:
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Generation of data from biological experiments
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Mathematical quantification of changes in cell components
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Creation of a model of the cellular system in silico
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Creation of a conceptual model based on philosophical techniques
Frage 61
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The symptom ‘chlorosis’ which develops when some fungi infect plants is due to:
Antworten
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Cell death
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Nutrient imbalance
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Impaired photosynthesis
-
Excess plant hormones
Frage 62
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The symptom ‘hyperplasia’ in plants caused by a fungal infection is due to:
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Abnormal cell enlargement
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Downward growth of petioles
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Uncontrolled cell division
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Restriction of water transport
Frage 63
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Which of the following is NOT a correct statement about true fungi
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True fungi are eukaryotic
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All true fungi are multi-cellular
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True fungi are heterotrophic
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Most true fungi have chitin cell walls
Frage 64
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Which of the following is NOT an alternative name for cutaneous fungal infections of humans:
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Superficial mycoses
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Dermatophytes
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Mycetoma
-
Ringworms
Frage 65
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Which of the following is NOT part of the plant disease triangle:
Antworten
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Susceptible host
-
Climate change
-
Active pathogen
-
Suitable environment
Frage 66
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Which of the following organisms is NOT a true fungus (i.e. it is classified in the Kingdom Protoctista):
Antworten
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Phytophthora infestans
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Botrytis cinerea
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Agaricus bisporus
-
Taphrina deformans
Frage 67
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Which of the following statements is NOT true about rust fungi?
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They are obligate pathogens
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They are host specific pathogens
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They have an active saprophytic phase
-
They form basidiospores as part of their life cycle
Frage 68
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Which of these statements is untrue?
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Some species of yeasts are highly tolerant to ethanol stress
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Some species of fungi are extremely xerophilic
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Many fungi are more tolerant to water stress than any bacterial species
-
Fungi can grow over the entire pH range from 0 to 14
Frage 69
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Zoophilic dermatophytes are:
Antworten
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normally associated with animals and humans
-
normally only associated with humans
-
mainly associated with the soil
-
mainly associated with contaminated water
Frage 70
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Modification of RNA polymerase confers bacterial resistance to:
Antworten
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Beta-lactam antibiotics
-
Rifampicin
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Quinolone antibiotics
-
Metronidazole
Frage 71
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The Bacille-Calmette-Guérin (BCG) vaccine is:
Antworten
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Heat-killed Mycobacterium tuberculosis
-
Attenuated live Mycobacterium tuberculosis
-
Mycobacterium bovis
-
Attenuated live Mycobacterium bovis
Frage 72
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The antibiotic rifampicin interferes with which of the following?
Antworten
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Peptidoglycan synthesis
-
DNA gyrase
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Lipid synthesis
-
RNA polymerase
Frage 73
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Which one of the following infectious diseases has been eradicated from the human population?
Antworten
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Plague
-
Leprosy
-
Small pox
-
Polio
Frage 74
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Which of the following is a bacterial infection?
Frage 75
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Which of the following is a host component important in specific immune recognition of extracellular microbes?
Antworten
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Antigen
-
Antibody
-
Phagocyte
-
Lipopolysaccharide
Frage 76
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Which of the following is NOT an acquired mechanism of antibiotic resistance in bacteria?
Antworten
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Beta-lactamase production
-
Efflux pump antibiotic export
-
Lack of peptidoglycan
-
RNA polymerase single nucleotide change
Frage 77
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Which of the following is only transmitted by direct person-to-person contact?
Antworten
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Malaria
-
Plague
-
Gonorrhoea
-
Tuberculosis
Frage 78
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Which of the following is transmitted via the faecal-oral-route?
Antworten
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Malaria
-
Typhoid
-
Plague
-
Meningitis
Frage 79
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Which of the following is primarily a respiratory pathogen?
Frage 80
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Which of the following is transmitted to humans by arthropods?
Antworten
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Tuberculosis
-
Malaria
-
Cholera
-
Typhoid
Frage 81
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Which one of the following diseases is NOT caused by an infectious agent?
Antworten
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Stomach ulcer
-
Cystic fibrosis
-
Cervical cancer
-
Liver cancer
Frage 82
Antworten
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Enter cells by receptor-mediated endocytosis
-
Replicate their genomes in the nucleus of the cell
-
Replicate their genomes in the cytoplasm of the cell
-
Surround their progeny genomes with capsid proteins inside infected cells
Frage 83
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Mature virus particles (Virions) ALWAYS contain:
Antworten
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non-structural proteins
-
a protease enzyme
-
a DNA or RNA genome
-
a reverse transcriptase
Frage 84
Antworten
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Has a double-stranded DNA genome
-
Has a capsid that is composed of 240 copies of a single protein
-
Replicates primarily in the upper respiratory tract
-
Belongs to the enterovirus genus of the picornavirus family
Frage 85
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The genome of measles virus is composed of :
Antworten
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Double stranded DNA
-
Single stranded DNA
-
Single stranded RNA
-
DNA and RNA
Frage 86
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The poliovirus mature virus particle:
Antworten
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Contains 240 copies of a single protein
-
Contains 60 copies of four proteins
-
Has a dsDNA genome
-
Has a single stranded negative–sense genome
Frage 87
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Tobacco mosaic virus has:
Frage 88
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Underline the TRUE statement below:
Antworten
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Viruses do not infect microorganisms
-
All viruses are enveloped
-
All viruses cause disease
-
All viruses are parasites
Frage 89
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Viruses may encode their genomes as
Antworten
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DNA
-
RNA
-
negative strand RNA
-
All of the above
Frage 90
Antworten
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Are always smaller than bacterial cells
-
Can replicate independently of a host cell if provided with a nutrient medium
-
Can use some of the enzymes expressed in the host cell to aid their replication
-
Are always eliminated by the host immune system