Microbiology Lab Practical

Question

What method of staining was used to create this slide?

Answer 1

Acid-fast Stain

Answer 2

Endospore Stain

Answer 3

Gram Stain

Answer 4

Negative Stain

Answer 5

Negative Stain

Answer 6

Endospore Stain

Answer 7

Gram Stain

Answer 8

Acid-fast Stain

Answer 9

Endospores

Answer 10

Mycobacterium

Answer 11

On the shelf under the lab bench.

Answer 12

On the floor next to your seat.

Answer 13

On the lab bench.

Answer 14

Safety Glasses

Answer 15

Closed toe shoes

Answer 16

Shoe covers

Answer 17

Disinfect on the way into the lab.

Answer 18

Disinfect on the way out of the lab.

Answer 19

Paper towels go in the regular trash.

Answer 20

Paper towels go in the autoclave trash.

Answer 21

Disinfect between each step of the lab process.

Answer 22

Disinfect your lab bench, take care of your need and then disinfect your lab bench, again.

Answer 23

Go to the corner of the lab room and take care of your need.

Answer 24

Alert the professor

Answer 25

Get the fire extinguisher near the front door

Answer 26

Get the fire extinguisher from the back of the room

Answer 27

Get deionized water from the back of the room and pour it on the fire

Answer 28

Alert the professor

Answer 29

Spray disinfectant on the spill

Answer 30

Spray disinfectant on a paper towel and place on the spill

Answer 31

Keep spraying disinfectant on the paper towel

Answer 32

Wait 10 minutes

Answer 33

Wait 15 minutes

Answer 34

Dispose in autoclave trash

Answer 35

Dispose in regular trash

Answer 36

Clean with dust pan and broom next to the fridge.

Answer 37

Clean with dust pan and broom near the front door.

Answer 38

Dispose of in the broken glass disposal box.

Answer 39

Dispose of in the autoclave trash.

Answer 40

Dispose of in the regular trash.

Answer 41

Use the eyewash located near the front door.

Answer 42

Use the eyewash located at the back of the room.

Answer 43

Use the eyewash next to the fridge.

Answer 44

Use the eyewash near back of the room.

Answer 45

In the autoclave trash

Answer 46

In the regular trash

Answer 47

In the used glove disposal bin

Answer 48

Compound and Electron.

Answer 49

Compound uses light and Electron uses electrons.

Answer 50

Compound and Bright Field.

Answer 51

Compound uses electrons and Bright Field uses light.

Answer 52

Compound uses light and Bright Field uses electrons.

Answer 53

Phase/Contrast Microscope

Answer 54

Bright Field Microscope

Answer 55

Dark Field Microscope

Answer 56

Fluorescent Microscope

Answer 57

Scanning Microscope

Answer 58

Objective Microscope

Answer 59

Magnification

Answer 60

Resolving power.

Answer 61

Refractive index.

Answer 62

Resolution.

Answer 63

Resolution

Answer 64

Refractive index

Answer 65

Resolving power

Answer 66

Magnification

Answer 67

Refractive index.

Answer 68

Resolution

Answer 69

Resolving power

Answer 70

Magnification

Answer 71

Numerical aperture

Answer 72

Wavelength of light

Answer 73

Diopter ring

Answer 74

Iris diaphragm

Answer 75

Diopter ring

Answer 76

Refractive index

Answer 77

Iris diaphragm

Answer 78

Diopter ring

Answer 79

Diopter ring

Answer 80

Diaphragm ring

Answer 81

Iris diaphragm

Answer 82

Condenser raised to the highest position

Answer 83

Iris and base diaphragm are open

Answer 84

Rheostat is turned up

Answer 85

Condenser is lowered to the lowest position

Answer 86

Numerical aperture

Answer 87

Wavelength of light

Answer 88

Rheostat level

Answer 89

Refractive index

Answer 90

Pure culture

Answer 91

Pure culture

Answer 92

Pure culture

Answer 93

Pure culture

Answer 94

Pure culture

Answer 95

Label a piece of labeling tape with your name and the name of the bacteria and place on test tube.

Answer 96

Use a wax pencil and label the test tube with your name and the name of the bacteria.

Answer 97

Use a marker and label the test tube with your name and the name of the bacteria.

Answer 98

Label the bottom of the media side using a wax pencil or marker with your name and the organism you are transferring.

Answer 99

Label the lid using a wax pencil or marker with your name and the name of the organism you are transferring.

Answer 100

Label a piece of labeling tape with your name and the name of the organism you are transferring and place it on the lid of the Petri plate.

Answer 101

Sterilize inoculating loop in Bacticinerator. Lightly drag the tip of the loop from side to side or back and forth in order to spread the inoculum. Replace lid. Incubate lid side up.

Answer 102

Sterilize inoculating loop in Bacticinerator. Lightly drag the tip of the loop from side to side or back and forth in order to spread the inoculum. Replace lid. Incubate lid side down.

Answer 103

Microorganisms are everywhere

Answer 104

Microorganisms are hard to identify

Answer 105

Microorganisms are difficult to see without a microscope

Answer 106

Microorganisms come in many forms

Answer 107

Aseptic technique

Answer 108

Sterile transfer

Answer 109

Inoculation technique

Answer 110

Ubiquitous

Answer 111

Bacteria have an overall negative charge so stain with positive (basic) dyes. Opposites attract.

Answer 112

Bacteria have an overall positive charge so stain with positive (basic) dyes. Likes attract likes.

Answer 113

Simple stain uses one dye; all bacteria are the same color; shows size, shape and arrangement. Differential stain uses two dyes; different bacteria are stained contrasting colors; shows size, shape, arrangement and something else.

Answer 114

Differential stain uses one dye; all bacteria are the same color; shows size, shape and arrangement. Simple stain uses two dyes; different bacteria are stained contrasting colors for simple identification; shows size, shape, arrangement and something else.

Answer 115

A) Gram negative bacteria B) Gram positive bacteria

Answer 116

A) Gram positive bacteria B) Gram negative bacteria

Answer 117

A) Gram stain B) Acid-fast stain

Answer 118

A) Acid-fast stain B) Gram stain

Answer 119

Insure proper staining

Answer 120

Insure proper decolorizing

Answer 121

The slide is small

Answer 122

Allows you to prepare only one or two bacteria.

Answer 123

Insure the cells stay intact and stain properly.

Answer 124

Insure the cells are dead and stain properly

Answer 125

Insure the cells wont move around during staining

Answer 126

It is the most critical step and there is no hard and fast rule on how long to decolorize.

Answer 127

It is the most critical step and don't want to drop the slides.

Answer 128

It is the most critical step and need to time it exactly.

Answer 129

1) Crystal Violet - Primary stain 2) Iodine - Mordant, combines with the crystal violet in the cell. 3) Acetone or ethanol or combination of 75% ethanol/25% acetone - Decolorizer 4) Safranin - Counterstain or Secondary stain

Answer 130

1) Crystal Violet - Primary stain 2) Safranin - Counterstain or Secondary stain 3) Iodine - Mordant, combines with the crystal violet in the cell. 4) Acetone or ethanol or combination of 75% ethanol/25% acetone - Decolorizer

Answer 131

1) Crystal Violet - Primary stain 2) Acetone or ethanol or combination of 75% ethanol/25% acetone - Decolorizer 3) Iodine - Mordant, combines with the crystal violet in the cell. 4) Safranin - Counterstain or Secondary stain

Answer 132

1) Crystal Violet - Primary Stain 2) Iodine - Mordant, combines with the crystal violet in the cell 3) Safranin - Counterstain or Secondary stain 4) Acetone or ethanol or combination of 75% ethanol/25% acetone - Decolorizer

Answer 133

The high lipid content in gram negative cell walls causes them to dissolve in the ethanol allowing the crystal violet dye to leak out.

Answer 134

The low lipid content in gram negative cell walls causes them to dissolve in the ethanol allowing the crystal violet dye to leak out.

Answer 135

It distinguishes between two groups of organisms based on the lipid content in their cell walls.

Answer 136

It uses two positively charged dyes.

Answer 137

It uses one positively charged dye and one negatively charged dye.

Answer 138

It tells size, shape, arrangement and something else.

Answer 139

It tells size, shape and arrangement.

Answer 140

Acid fast uses a solution of 3% HCl and 95% ethanol. Gram stain uses just ethanol.

Answer 141

Acid fast uses a solution of 95% HCl and 3% ethanol. Gram stain uses just ethanol.

Answer 142

Ziehl-Neelsen uses steam to penetrate the mycolic acid in cell walls. Kinyoun uses concentrated dye, hard heat fixing and longer exposure time.

Answer 143

Kinyoun uses steam to penetrate the mycolic acid in cell walls. Ziehl-Neelsen uses concentrated dye, hard heat fixing and longer exposure time.

Answer 144

Ziehl-Neelsen uses hard heat fixing to penetrate the mycolic acid in cell walls. Kinyoun uses concentrated dye, steam and longer exposure time.

Answer 145

Kinyoun uses hard heat fixing to penetrate the mycolic acid in cell walls. Ziehl-Neelsen uses concentrated dye, steam and longer exposure time.

Answer 146

Mycobacterium tuberculosis Disease: Tuberculosis

Answer 147

Mycobacterium leprae Disease: Leprosy or Hansen's disease

Answer 148

Nocardia asteroides Disease: Nocardiosis

Answer 149

Cryptosporidium Disease: Cryptosporidiosis

Answer 150

Mycosporidium Disease: Mycardiosis

Answer 151

Nocardia tuberculosis Disease: Tuberculosis

Answer 152

1) Carbolfuchsin 2) Acid-alcohol 3) Loeffler's methylene blue

Answer 153

1) Loeffler's methylene blue 2) Acid-alcohol 3) Carbolfichsin

Answer 154

1) Carbolfuschsin 2) Iodine 3) Acid-alcohol 4) Loeffler's methylene blue

Answer 155

1) Loeffler's methylene blue 2) Iodine 3) Acid-alcohol 4) Carbolfuchsin

Answer 156

Steam for 3-5 minutes

Answer 157

Hard heat fixing then letting the dye remain on the slide for an extended period of time.

Answer 158

Hard heat fixing

Answer 159

Steam then letting the dye remain on teh slide for an extended period of time.

Answer 160

Malachite green

Answer 161

Gram's safranin

Answer 162

Loeffler's methylene blue

Answer 163

Vegetative cell

Answer 164

Germinated cell

Answer 165

Sporogenesis

Answer 166

Decolorization

Answer 167

Germination

Answer 168

Germination

Answer 169

Sporogenesis

Answer 170

Can remain dormant indefinitely

Answer 171

Resistant to antibiotics and most disinfectants

Answer 172

Resistant to radiation

Answer 173

Resistant to boiling

Answer 174

Resistant to drying

Answer 175

Impermeability of spore coat

Answer 176

Bacillus anthracis Disease: anthrax

Answer 177

Clostridium botulinum Disease: Botulism

Answer 178

Clostridium difficile Disease: Toxic enterocolotis

Answer 179

Clostridium perfringens Disease: Gas gangrene, food poisoning

Answer 180

Clostridium tetani Disease: Tetnus, lockjaw

Answer 181

Clostridium anthracis Disease: Anthrax

Answer 182

Clostridium

Answer 183

Streptococcus

Answer 184

Staphylococcus

Answer 185

Stains what you want to see Stains everything but the structure you want to see

Answer 186

Stains everything but the structure you want to see Stains what you want to see

Answer 187

Stains gram (+) bacteria Stains gram (-) bacteria

Answer 188

Stains gram (-) bacteria Stains gram (+) bacteria

Answer 189

They are negatively charged dyes and are repelled by the negative contents of the bacterial cell.

Answer 190

They are positively charged dyes and are repelled by the positive contents of the bacterial cell.

Answer 191

They are positively charged dyes and are attracted by the negative contents of the bacterial cell.

Answer 192

They are negatively charged dyes and are attracted by the positive contents of the bacterial cell.

Answer 193

Able to see a capsule

Answer 194

Do not heat fix so can see them as they are

Answer 195

Can be used for things that don't stain.

Answer 196

Can see if it produces endospores

Answer 197

Can see if it is gram (-) or gram (+)

Answer 198

Slime layer

Answer 199

Slime layer

Answer 200

Helps the bacteria avoid phagocytosis by white blood cells

Answer 201

Helps the bacteria attach to skin and mucous membranes

Answer 202

Is a source of nutrition for the bacteria when resources are scarce

Answer 203

Contains the pathogenic part of the bacteria

Answer 204

A smear for a capsule stain is not heat fixed.

Answer 205

A spear from a capsule stain is not rinsed with deionized water it is rinsed with copper sulfate

Answer 206

A smear for a capsule stain uses steam

Answer 207

A smear for a Gram stain uses crystal violet and a capsule stain does not

Answer 208

Dark blue Light blue to white

Answer 209

Light blue to white Dark blue

Answer 210

Superoxides

Answer 211

Carbon dioxide

Answer 212

Carbon monoxide

Answer 213

Peroxidase

Answer 214

Superoxide dismutase

Answer 215

The flame burns using up the oxygen and producing CO2

Answer 216

The flame burns up the CO2 and produces oxygen.

Answer 217

The pak contains chemicals that produce H+ and CO2. The H+ combines with the O2 in the jar to produce H2O.

Answer 218

The pak contains chemicals that produce CO2 and destroy O2.

Answer 219

Facultative anaerobes

Answer 220

Microaerophiles

Answer 221

Capnophiles

Answer 222

Aerotolerant anaerobes

Answer 223

The agar prevents diffusion of O2

Answer 224

It contains a reducing agent, sodium thioglycollate, that combines with O2 to produce water

Answer 225

It contains a reducing agent, sodium thioglycollate, that combines with the agar to produce CO2 near the bottom

Answer 226

The agar prevents diffusion of CO2

Answer 227

Strict aerobe

Answer 228

Facultative anaerobe

Answer 229

Only at the top of the thioglycollate broth.

Answer 230

Candle jar

Answer 231

Capnophile

Answer 232

Microaerophile

Answer 233

Facultative anaerobe

Answer 234

Grows in candle jar

Answer 235

Grows at the point where the oxygen level is diminishing in the thio broth.

Answer 236

Grows in the GasPak

Answer 237

Capnophile

Answer 238

Microaerophile

Answer 239

Grows in candle jar

Answer 240

Grows in oxygen

Answer 241

Grows in a GasPak

Answer 242

Facultative anaerobe

Answer 243

Microaerophile

Answer 244

Grows in oxygen

Answer 245

Grows in candle jar

Answer 246

Grows in GasPak

Answer 247

Grows throughout the entire thio broth slant

Answer 248

Aerotolerant anaerobe

Answer 249

Microaerophile

Answer 250

All over the thio broth slant but slightly less at the top

Answer 251

Grows in the GasPak

Answer 252

Capnophile

Answer 253

Strict anaerobe

Answer 254

Grows in GasPak

Answer 255

Grows in candle jar

Answer 256

Grows toward the bottom of the thio broth

Answer 257

Grows toward the top of the thio broth

Answer 258

Green fluorescent protein

Answer 259

Fluorescent blue plasmid

Answer 260

Competent cells

Answer 261

Competent genes

Answer 262

Competent proteins

Answer 263

Mixing cells with calcium chloride during growth phase

Answer 264

Exposing cells to extreme temperature changes during the proceduree

Answer 265

Mixing cells with with heated calcium hydroxide

Answer 266

Freezing cells

Answer 267

A gene is a piece of DNA that provides the instructions for making a protein

Answer 268

A protein is a piece of DNA that provides the instructions for making a gene

Answer 269

DNA is a piece of a protein that provides the instructions for making a gene

Answer 270

Carries genes needed for the hereditary characteristics essential for bacterial growth and reproduction. Small pieces of circular DNA that have genes for one or more traits that may be beneficial but are not essential for bacterial survival.

Answer 271

Small pieces of circular DNA that have genes for one or more traits that may be beneficial but are not essential for bacterial survival. Carries genes needed for the hereditary characteristics essential for bacterial growth and reproduction.

Answer 272

Carries genes needed for the hereditary characteristics that may be beneficial but are not essential for bacterial growth and reproduction. Small pieces of circular DNA that have genes for one or more traits that are essential for bacterial survival.

Answer 273

The Ara-C gene controls expression of GFP via the sugar arabinose

Answer 274

The Ara-B gene controls expression of GFP via the sugar arabinose

Answer 275

The Ara-C gene controls expression of GFP via the sugar glucose

Answer 276

Antibiotic

Answer 277

Antibiotic

Answer 278

Antibiotic

Answer 279

Contributes to escalating drug resistance

Answer 280

Wipes out a person's normal flora resulting in superinfections

Answer 281

Only works on weak bacteria

Answer 282

An organism is susceptible to drug concentrations obtained in the body using normal therapeutic doses.

Answer 283

The antibiotic is likely to be effective against the pathogen

Answer 284

The antibiotic is not likely to work.

Answer 285

Stability of the antibiotic

Answer 286

Rate of diffusion of the antibiotic

Answer 287

pH of the culture medium

Answer 288

Depth of the culture medium

Answer 289

Inoculum density

Answer 290

Incubation time

Answer 291

Incubation temperature

Answer 292

Concentration of antibiotic

Answer 293

Power switch

Answer 294

Coarse focus knob

Answer 295

Power switch

Answer 296

Coarse focus knob

Answer 297

Power switch

Answer 298

Coarse focus knob

Answer 299

Fine focus knob

Answer 300

Coarse focus knob

Answer 301

Fine focus knob

Answer 302

Power switch

Answer 303

Fine focus knob

Answer 304

Coarse focus knob

Answer 305

Power switch

Answer 306

Diaphragm ring

Answer 307

Fine focus knob

Answer 308

Mechanical stage

Answer 309

Slide stage

Answer 310

Ocular stage

Answer 311

Fine focus knob

Answer 312

Coarse focus knob

Answer 313

Mechanical stage

Answer 314

Mechanical base

Answer 315

A - Objective lens B - Iris diaphragm lever C - Condenser centering screw D - Base diaphragm E - Mechanical stage knob

Answer 316

A - Ocular lens B - Iris diaphragm lever C - Condenser centering screw D - Base diaphragm E - Mechanical stage knob

Answer 317

A - Ocular lens B - Condenser centering screw C - Iris diaphragm lever D - Base diaphragm E - Mechanical stage knob

Answer 318

A - Ocular lens B - Iris diaphragm lever C - Mechanical stage knob D - Base diaphragm E - Condenser centering screw

Answer 319

A - Iris diaphragm lever B - Condenser centering screw C - Base diaphragm

Answer 320

A - Condenser centering screw B - Iris diaphragm lever C - Base diaphragm

Answer 321

A - Iris diopter screw B - Iris diaphragm lever C - Base diaphragm

Answer 322

A - Iris diopter lever B - Condenser centering screw C - Base diaphragm

Answer 323

A - Revolving nose piece B - Mechanical stage clip

Answer 324

A - Objective ring B - Mechanical stage clip

Answer 325

A - Diopter ring B - Mechanical stage clip

Answer 326

A - Condenser knob B - Condenser C - Base diaphragm ring

Answer 327

A - Condenser knob B - Condenser ring C - Base

Answer 328

A - Condenser ring B - Condenser C - Base

Answer 329

Microaerophile

Answer 330

Obligate/Strict aerobe

Answer 331

Facultative anaerobe

Answer 332

Obigate/strict anaerbe

Answer 333

Microaerophile

Answer 334

Obligate/Strict aerobe

Answer 335

Facultative anaerobe

Answer 336

Obligate anaerobe

Answer 337

Microaerophile

Answer 338

Obligate/strict aerobe

Answer 339

Facultative anaerobe

Answer 340

Obligate/strict anaerobe

Answer 341

Microaerophile

Answer 342

Obligate/strict aerobe

Answer 343

Facultative anaerobe

Answer 344

Obligate/strict anaerobe

Answer 345

Because E.coli are sensitive to Ampicillin

Answer 346

Because E. coli are resistant to Ampicillin

Answer 347

Because luria does not contain the nutrients necessary for growth.

Answer 348

Beta lactamase

Answer 349

Green fluorescent protein

Answer 350

Beta lactamase

Answer 351

Green fluorescent protein

Answer 352

Beta lactamase

Answer 353

Green fluorescent protein

Answer 354

Mueller hinton

Answer 355

Kirby Bauer

Answer 356

It is pink and therefore, the test is negative and the microbe is sensitive to penicillin

Answer 357

it is pink and therefore, the test is positive and the microbe is sensitive to penicillin

Answer 358

it is pink and therefore, the test is positive and the microbe is resistant to penicillin

Answer 359

It is pink and therefore, the test is negative and the microbe is resistant to penicillin

Answer 360

It is yellow and therefore, the test is positive and the microbe is resistant to penicillin

Answer 361

It is yellow and therefore, the test is negative and the microbe is resistant to penicillin

Answer 362

It is yellow and therefore, the test is positive and the microbe is sensitive to penicillin

Answer 363

It is yellow and therefore, the test is negative and the microbe is sensitive to penicillin

Answer 364

Has agar in it and contains a reducing agent that produces water

Answer 365

Has agar and a reducing agent that produces CO2

Answer 366

Labeled on the bottom in case you lose the lid and inverted during incubation to prevent condensation from dripping onto the agar surface

Answer 367

Labeled on the bottom in case you lose the lid and inverted so you can read the label

Answer 368

So you do not contaminate the cap.

Answer 369

So the cap doesn't roll away.

Answer 370

So you can stretch your fingers.

Answer 371

Green fluorescent protein

Answer 372

Beta lactamase

Answer 373

Ara-C represser protein

Answer 374

Beta green protein

Answer 375

Does the bacteria have the BLA gene and therefore produce beta lactamase

Answer 376

Does the bacteria have the BLA protein and therefore produce the beta lactamase gene

Answer 377

Arabinose has to move the Ara-C represser protein out of the way so GFP can be transcribed and translated.

Answer 378

GFP takes up the arabinose so Ara-C can be transcribed and translated.

Answer 379

Sodium thioglycollate is a reducing agent and it reduces by binding O2 and converting it to water

Answer 380

Sodium thioglycollate is a reducing agent that reduces by causing evaporation of the broth

Answer 381

Whole colony

Answer 382

Near the front door: Fire extinguisher, Eye wash, Shower, Gloves

Answer 383

Back of room: Fume hood

Answer 384

Lab bench: Disinfectant, Autoclave bags, Paper towels

Answer 385

Near Sinks: Bleach, Dyes used for stains, Paper towels (above)

Answer 386

Fume hood: Broken glass disposal box (underneath), Autoclave bags

Answer 387

Front podium: Phone

Answer 388

Back: Test tube racks, MSDS notebooks (back corner),

Answer 389

Next to fridge: Dustpan and broom

Answer 390

Used glass test tubes

Answer 391

Used glass slides

Answer 392

Broken glass

Answer 393

Used latex gloves

Answer 394

Paper towels used for bench disinfection

Answer 395

Contaminated Petri plates

Answer 396

Used glass slides

Answer 397

Used glass test tubes

Answer 398

Contaminated Petri plates

Answer 399

Broken glass

Answer 400

Broken glass

Answer 401

Paper towels used in bench disinfection

Answer 402

Qtip swabs used to clean microscopes

Answer 403

Used latex gloves

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Microbiology Lab Practical

Beschreibung

Zusammenfassung der Ressource

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