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Beschreibung
Karteikarten von Charlotte Hewson, aktualisiert more than 1 year ago
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Erstellt von Charlotte Hewson
vor mehr als 8 Jahre
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| Frage | Antworten |
| what is used to locate specific genes? | DNA Probes and DNA hybridisation |
| what is a DNA Probe? | a short, single stranded section of DNA with some form of label to identify it easily |
| what are the two most common probes? | radioactively labelled probes fluorescently labelled probes |
| what is a radioactive probe made up of? | nucleotides with the isotope 32P |
| how is it identified? | using a photographic plate that is exposed by radioactivity |
| how do you detect a fluorescent probe? | it emits light under certain conditions |
| how are they used? | - probe has complementary bases to sequence of wanted gene, - DNA separated into two strands - separated DNA strands mixed with probe, binds to complementary bases -site of binding can be identified by marker |
| what is it called when the DNA binds with the Probe? | DNA Hybridisation |
| what needs to be done before making the probe? | find the sequence of DNA of the gene you want to locate |
| what method is used? | the sanger method |
| how many test tubes are set up in this method? | 4 |
| what goes in each one? | - single strands of DNA to be sequenced, acts as template for synthesis of complementary strand |
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