Proteomics (2)

Proteomics (2)

1. PANCREATIC DUCTAL ADENOCARCINOMA (PDA)

   • intro
     • PDA accounts for 90% of all pancreatic cancers
     • Pancreatic cancer is the 4th most common cause of cancer-related deaths
     • 5-year survival is only 10-24%
     • PDA arises from ductal cells of the pancreas
   • Method of detection
     • Laser capture microscopy
       • use a laser to accurately cut out only the part of the tissue sample that is to be analysed. e.g. te ductal cells and discard the remaining tissue around it
       • Analyse the different spots observed between diseased samples and healthy controls using tissue array. e.g. Western blot
       • Identify the spots found only in disease tissues using mass spectroscopy
       • Validate the process
         • tissue arrays
         • linkeage analysis
   • S100A6 (Calcylin) identified as being associated with poor survival in PDA

2. PROSTATE CANCER

   • intro
     • Most common male cancer in the Western world
     • 3rd leading cause of cancer deaths among men
     • currently the diagnosis involves
       • PSA measurements
       • digital rectal exams
       • transrectal ultrasound guided sonography
     • over-diagnosis and over-treatment
     • treatment based on whether disease is organ confined
   • Detection
     • literature review
     • sample collection and preparation
     • protein separation by 2-DE DIGE
     • gel image analysis to look for any spots in diseased gel not in healthy control gel
     • protein identification using MS
     • Validation using MRM techniques
     • Enter text here