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7328029
Culturing Microorganisms
Description
GCSE CGP Biology Mind Map on Culturing Microorganisms, created by Z S on 07/01/2017.
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cgp biology
gcse
Mind Map by
Z S
, updated more than 1 year ago
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Created by
Z S
over 7 years ago
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Resource summary
Culturing Microorganisms
You can grow bacteria in the lab
Bacteria are cultured in a culture medium
This contains the carbohydrates, minerals, proteins and vitamins they need to grow
Can be a nutrient broth solution or solid agar jelly
Bacteria grown on agar plates will form visible colonies on the surface of the jelly, or will spread out to give an even covering of bacteria
To make an agar plate, hot agar jelly is poured into shallow round plastic dishes called Petri dishes
When the jelly's cooled and set, inoculating loops can be used to transfer microorganisms to the culture medium
The microorganisms then multiply
In the lab at school, cultures of microorganisms are not kept above 25 C
Because harmful pathogens are more likely to grow above this temperature
In industrial conditions, cultures are incubated at higher temperatures so that they can grow a lot faster
You need to use uncontaminated cultures
Contamination by unwanted microorganisms will affect the results
It can also potentially result in the growth of pathogens
The Petri dishes and culture medium must be sterilised before use to kill any unwanted microorganisms that may be lurking on them
If an inoculating loop is used to transfer the bacteria to the culture medium, it should be sterilised first by passing it through a hot flame
After transferring the bacteria, the lid of the Petri dish should be lightly taped on
To stop microorganisms from the air getting in
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