Why should null alleles be used for epistatic analysis (i.e how to find out the order of gene function in a pathway)
Answer
The masking of alleles downstream becomes obvious
They are easier to make than hypomorphic alleles
They can be used for both Substrate Dependent and Switch pathways
Question 12
Question
How do we identify that the pathway effected by the (epistatic) mutation is a Switch Regulatory pathway?
Answer
If the mutation prevents the production of the final product
If the phenotype of a double mutant is different to the phenotype of either single mutant
Question 13
Question
The Rif1 & Rif2 strains are both causes lengthened telomeres, as shown in the diagram. Based on the Rif1/Rif2 double mutation, which of the two availible pathways (shown) is likely to be the one used?