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Microbiology Lab Practical

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MCC Microbiology Lab Practical 2
Elle Ashe
Test por Elle Ashe, actualizado hace más de 1 año
Elle Ashe
Creado por Elle Ashe hace más de 10 años
1802
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Resumen del Recurso

Pregunta 1

Pregunta
What method of staining was used to create this slide?
Image:
2_d7085 (image/jpg)
Respuesta
  • Acid-fast Stain
  • Endospore Stain
  • Gram Stain
  • Negative Stain

Pregunta 2

Pregunta
What kind of staining process was used to make this slide?
Image:
2_Micro_Bacteria_Capsule_Stain (image/jpg)
Respuesta
  • Negative Stain
  • Endospore Stain
  • Gram Stain
  • Acid-fast Stain

Pregunta 3

Pregunta
What kind of microbes are present on this slide?
Image:
2_Gram_negative_cocci (image/jpg)
Respuesta
  • Gram (-)
  • Endospores
  • Nocardia
  • Mycobacterium

Pregunta 4

Pregunta
Where should your backpacks and purses be stored during the lab period?
Respuesta
  • On the shelf under the lab bench.
  • On the floor next to your seat.
  • On the lab bench.

Pregunta 5

Pregunta
What items should you wear to protect yourself in the lab.
Respuesta
  • Lab coat
  • Safety Glasses
  • Gloves
  • Closed toe shoes
  • Hair net
  • Shoe covers

Pregunta 6

Pregunta
When do you disinfect your lab table and where do you discard the used paper towels?
Respuesta
  • Disinfect on the way into the lab.
  • Disinfect on the way out of the lab.
  • Paper towels go in the regular trash.
  • Paper towels go in the autoclave trash.
  • Disinfect between each step of the lab process.

Pregunta 7

Pregunta
You are dying of thirst (hunger, for smoke, chapped lips, bad breath) during the lab period. What do you do to alleviate your problem?
Respuesta
  • Leave
  • Disinfect your lab bench, take care of your need and then disinfect your lab bench, again.
  • Go to the corner of the lab room and take care of your need.

Pregunta 8

Pregunta
You notice your lab partner has caught the lab manual on fire. What should you do?
Respuesta
  • Alert the professor
  • Get the fire extinguisher near the front door
  • Get the fire extinguisher from the back of the room
  • Get deionized water from the back of the room and pour it on the fire

Pregunta 9

Pregunta
Your lab partner has managed to spill microorganisms all over the lab table. What should be done to clean up the spill?
Respuesta
  • Alert the professor
  • Spray disinfectant on the spill
  • Spray disinfectant on a paper towel and place on the spill
  • Keep spraying disinfectant on the paper towel
  • Wait 10 minutes
  • Wait 15 minutes
  • Dispose in autoclave trash
  • Dispose in regular trash

Pregunta 10

Pregunta
A student broke an empty glass test tube. What should be done to clean up the glass and where should the pieces of glass be disposed of?
Respuesta
  • Clean with dust pan and broom next to the fridge.
  • Clean with dust pan and broom near the front door.
  • Dispose of in the broken glass disposal box.
  • Dispose of in the autoclave trash.
  • Dispose of in the regular trash.

Pregunta 11

Pregunta
The instructor tripped on a backpack on the floor knocking her safety goggles off and splashing HCl in her eyes. What should she do?
Respuesta
  • Use the eyewash located near the front door.
  • Use the eyewash located at the back of the room.
  • Use the eyewash next to the fridge.
  • Use the eyewash near back of the room.

Pregunta 12

Pregunta
After surviving the lab period you remove your gloves by turning them inside out and then where do you toss them?
Respuesta
  • In the autoclave trash
  • In the regular trash
  • In the used glove disposal bin

Pregunta 13

Pregunta
Choose the two major types of microscopes that have been developed and the difference between them
Respuesta
  • Compound and Electron.
  • Compound uses light and Electron uses electrons.
  • Compound and Bright Field.
  • Compound uses electrons and Bright Field uses light.
  • Compound uses light and Bright Field uses electrons.

Pregunta 14

Pregunta
Which microscopes are compound microscopes?
Respuesta
  • Phase/Contrast Microscope
  • Bright Field Microscope
  • Dark Field Microscope
  • Fluorescent Microscope
  • Scanning Microscope
  • Objective Microscope

Pregunta 15

Pregunta
The ability of the microscope to enlarge an object.
Respuesta
  • Magnification
  • Resolving power.
  • Refractive index.
  • Resolution.

Pregunta 16

Pregunta
How clear your specimen will appear when viewed through the lenses.
Respuesta
  • Resolution
  • Refractive index
  • Resolving power
  • Magnification

Pregunta 17

Pregunta
How well you lens is able to bend light.
Respuesta
  • Refractive index.
  • Resolution
  • Resolving power
  • Magnification

Pregunta 18

Pregunta
What components influence the resolving power of a microscope?
Respuesta
  • Numerical aperture
  • Wavelength of light
  • Condenser
  • Diopter ring

Pregunta 19

Pregunta
Concentrates the light beam and directs light through the specimen.
Respuesta
  • Condenser
  • Iris diaphragm
  • Diopter ring
  • Refractive index

Pregunta 20

Pregunta
Regulates the intensity of the light entering the lens.
Respuesta
  • Iris diaphragm
  • Condenser
  • Diopter ring
  • Rheostat

Pregunta 21

Pregunta
Focuses the individual eye piece to your eye.
Respuesta
  • Diopter ring
  • Rheostat
  • Diaphragm ring
  • Iris diaphragm

Pregunta 22

Pregunta
Steps to achieve adequate lighting when the oil immersion objective is used to view a specimen.
Respuesta
  • Condenser raised to the highest position
  • Iris and base diaphragm are open
  • Rheostat is turned up
  • Condenser is lowered to the lowest position

Pregunta 23

Pregunta
Components that influence the resolving power of a microscope.
Respuesta
  • Numerical aperture
  • Wavelength of light
  • Rheostat level
  • Refractive index

Pregunta 24

Pregunta
The isolation of a single species of microbe.
Respuesta
  • Pure culture
  • Colony
  • Media
  • Inoculum
  • Turbdity

Pregunta 25

Pregunta
Cells from a single asexual parent that are identical.
Respuesta
  • Colony
  • Pure culture
  • Media
  • Inoculum
  • Turbidity

Pregunta 26

Pregunta
Nutrient material suitable for the cultivation of microorganisms.
Respuesta
  • Media
  • Pure culture
  • Colony
  • Turbdity
  • Inoculum

Pregunta 27

Pregunta
Transfer of a pure culture from a slant culture to a new sterile broth. The sampling of a bacterial culture.
Respuesta
  • Inoculum
  • Pure culture
  • Colony
  • Media
  • Turbidity

Pregunta 28

Pregunta
Cloudiness in broth that indicates growth.
Respuesta
  • Turbidity
  • Pure culture
  • Media
  • Colony
  • Inoculum

Pregunta 29

Pregunta
Steps involved in labeling a test tube.
Respuesta
  • Label a piece of labeling tape with your name and the name of the bacteria and place on test tube.
  • Use a wax pencil and label the test tube with your name and the name of the bacteria.
  • Use a marker and label the test tube with your name and the name of the bacteria.

Pregunta 30

Pregunta
What are the steps for labeling a Petri plate?
Respuesta
  • Label the bottom of the media side using a wax pencil or marker with your name and the organism you are transferring.
  • Label the lid using a wax pencil or marker with your name and the name of the organism you are transferring.
  • Label a piece of labeling tape with your name and the name of the organism you are transferring and place it on the lid of the Petri plate.

Pregunta 31

Pregunta
What are the steps for incubating a Petri plate?
Respuesta
  • Sterilize inoculating loop in Bacticinerator. Lightly drag the tip of the loop from side to side or back and forth in order to spread the inoculum. Replace lid. Incubate lid side up.
  • Sterilize inoculating loop in Bacticinerator. Lightly drag the tip of the loop from side to side or back and forth in order to spread the inoculum. Replace lid. Incubate lid side down.

Pregunta 32

Pregunta
What is meant by the statement that microorganisms are ubiquitous?
Respuesta
  • Microorganisms are everywhere
  • Microorganisms are hard to identify
  • Microorganisms are difficult to see without a microscope
  • Microorganisms come in many forms

Pregunta 33

Pregunta
Handling of bacteria in a manner so as to prevent contaminating laboratory media and to prevent infecting ourselves and our classmates.
Respuesta
  • Aseptic technique
  • Sterile transfer
  • Inoculation technique
  • Ubiquitous

Pregunta 34

Pregunta
Why are "basic" dyes used in the Gram stain?
Respuesta
  • Bacteria have an overall negative charge so stain with positive (basic) dyes. Opposites attract.
  • Bacteria have an overall positive charge so stain with positive (basic) dyes. Likes attract likes.

Pregunta 35

Pregunta
State two differences between a simple stain and a differential stain.
Respuesta
  • Simple stain uses one dye; all bacteria are the same color; shows size, shape and arrangement. Differential stain uses two dyes; different bacteria are stained contrasting colors; shows size, shape, arrangement and something else.
  • Differential stain uses one dye; all bacteria are the same color; shows size, shape and arrangement. Simple stain uses two dyes; different bacteria are stained contrasting colors for simple identification; shows size, shape, arrangement and something else.

Pregunta 36

Pregunta
A) Has a high lipid content and thin layer of tecoic acid underneath and stains pink. B) Has a low lipid content and thick layer containing tecoic acids and stains purple.
Respuesta
  • A) Gram negative bacteria B) Gram positive bacteria
  • A) Gram positive bacteria B) Gram negative bacteria
  • A) Gram stain B) Acid-fast stain
  • A) Acid-fast stain B) Gram stain

Pregunta 37

Pregunta
What us the importance of using only a pinpoint of inoculum for the smear preparation?
Respuesta
  • Insure proper staining
  • Insure proper decolorizing
  • The slide is small
  • Allows you to prepare only one or two bacteria.

Pregunta 38

Pregunta
What is the importance of allowing the smear to air dry?
Respuesta
  • Insure the cells stay intact and stain properly.
  • Insure the cells are dead and stain properly
  • Insure the cells wont move around during staining

Pregunta 39

Pregunta
What is the importance of decolorizing smears one at a time?
Respuesta
  • It is the most critical step and there is no hard and fast rule on how long to decolorize.
  • It is the most critical step and don't want to drop the slides.
  • It is the most critical step and need to time it exactly.

Pregunta 40

Pregunta
List the gram stain reagents in order and state their function.
Respuesta
  • 1) Crystal Violet - Primary stain 2) Iodine - Mordant, combines with the crystal violet in the cell. 3) Acetone or ethanol or combination of 75% ethanol/25% acetone - Decolorizer 4) Safranin - Counterstain or Secondary stain
  • 1) Crystal Violet - Primary stain 2) Safranin - Counterstain or Secondary stain 3) Iodine - Mordant, combines with the crystal violet in the cell. 4) Acetone or ethanol or combination of 75% ethanol/25% acetone - Decolorizer
  • 1) Crystal Violet - Primary stain 2) Acetone or ethanol or combination of 75% ethanol/25% acetone - Decolorizer 3) Iodine - Mordant, combines with the crystal violet in the cell. 4) Safranin - Counterstain or Secondary stain
  • 1) Crystal Violet - Primary Stain 2) Iodine - Mordant, combines with the crystal violet in the cell 3) Safranin - Counterstain or Secondary stain 4) Acetone or ethanol or combination of 75% ethanol/25% acetone - Decolorizer

Pregunta 41

Pregunta
Why do gram negative cells lose the primary dye during decolorization?
Respuesta
  • The high lipid content in gram negative cell walls causes them to dissolve in the ethanol allowing the crystal violet dye to leak out.
  • The low lipid content in gram negative cell walls causes them to dissolve in the ethanol allowing the crystal violet dye to leak out.

Pregunta 42

Pregunta
Why is the Acid-fast stain considered a "differential stain"?
Respuesta
  • It distinguishes between two groups of organisms based on the lipid content in their cell walls.
  • It uses two positively charged dyes.
  • It uses one positively charged dye and one negatively charged dye.
  • It tells size, shape, arrangement and something else.
  • It tells size, shape and arrangement.

Pregunta 43

Pregunta
What is the difference between the decolorizer used in the Gram stain and the decolorizer used in the Acid-fast stain?
Respuesta
  • Acid fast uses a solution of 3% HCl and 95% ethanol. Gram stain uses just ethanol.
  • Acid fast uses a solution of 95% HCl and 3% ethanol. Gram stain uses just ethanol.

Pregunta 44

Pregunta
Lipids in the cell's waxy wall prevent the acid-alcohol from decolorizing the cell.
Respuesta
  • Acid-fast
  • Gram
  • Negative
  • Endospore

Pregunta 45

Pregunta
What is the difference between the Ziehl-Neelsen stain and the Kinyoun stain?
Respuesta
  • Ziehl-Neelsen uses steam to penetrate the mycolic acid in cell walls. Kinyoun uses concentrated dye, hard heat fixing and longer exposure time.
  • Kinyoun uses steam to penetrate the mycolic acid in cell walls. Ziehl-Neelsen uses concentrated dye, hard heat fixing and longer exposure time.
  • Ziehl-Neelsen uses hard heat fixing to penetrate the mycolic acid in cell walls. Kinyoun uses concentrated dye, steam and longer exposure time.
  • Kinyoun uses hard heat fixing to penetrate the mycolic acid in cell walls. Ziehl-Neelsen uses concentrated dye, steam and longer exposure time.

Pregunta 46

Pregunta
Which are acid-fast organisms and what disease do they cause?
Respuesta
  • Mycobacterium tuberculosis Disease: Tuberculosis
  • Mycobacterium leprae Disease: Leprosy or Hansen's disease
  • Nocardia asteroides Disease: Nocardiosis
  • Cryptosporidium Disease: Cryptosporidiosis
  • Mycosporidium Disease: Mycardiosis
  • Nocardia tuberculosis Disease: Tuberculosis

Pregunta 47

Pregunta
What are the reactants in the acid-fast stain, in order?
Respuesta
  • 1) Carbolfuchsin 2) Acid-alcohol 3) Loeffler's methylene blue
  • 1) Loeffler's methylene blue 2) Acid-alcohol 3) Carbolfichsin
  • 1) Carbolfuschsin 2) Iodine 3) Acid-alcohol 4) Loeffler's methylene blue
  • 1) Loeffler's methylene blue 2) Iodine 3) Acid-alcohol 4) Carbolfuchsin

Pregunta 48

Pregunta
Two methods used to force the dye into an endospore.
Respuesta
  • Steam for 3-5 minutes
  • Hard heat fixing then letting the dye remain on the slide for an extended period of time.
  • Hard heat fixing
  • Steam then letting the dye remain on teh slide for an extended period of time.

Pregunta 49

Pregunta
What dyes are used in the Schaffer-Fulton spore stain?
Respuesta
  • Malachite green
  • Gram's safranin
  • Iodine
  • Loeffler's methylene blue

Pregunta 50

Pregunta
In the "Christmas tree" stain, the endospores are colored _________________ and the vegetative cells are colored ____________________.
Respuesta
  • Green Red
  • Red Green

Pregunta 51

Pregunta
Metabolically active, replicating cell.
Respuesta
  • Vegetative cell
  • Endospore
  • Germinated cell

Pregunta 52

Pregunta
The formation of endospores within a vegetative cell
Respuesta
  • Sporogenesis
  • Decolorization
  • Germination

Pregunta 53

Pregunta
When an endospore reverts to a vegetative state.
Respuesta
  • Germination
  • Sporogenesis
  • Growth

Pregunta 54

Pregunta
Why are endospores considered "the most resistant life form known"?
Respuesta
  • Can remain dormant indefinitely
  • Resistant to antibiotics and most disinfectants
  • Resistant to radiation
  • Resistant to boiling
  • Resistant to drying
  • Impermeability of spore coat

Pregunta 55

Pregunta
What are the medically important endospore-producing bacteria and their associated disease?
Respuesta
  • Bacillus anthracis Disease: anthrax
  • Clostridium botulinum Disease: Botulism
  • Clostridium difficile Disease: Toxic enterocolotis
  • Clostridium perfringens Disease: Gas gangrene, food poisoning
  • Clostridium tetani Disease: Tetnus, lockjaw
  • Clostridium anthracis Disease: Anthrax

Pregunta 56

Pregunta
Which medically important genera of bacteria produce endospores?
Respuesta
  • Bacillus
  • Clostridium
  • Streptococcus
  • Staphylococcus

Pregunta 57

Pregunta
A positive stain _____________________________ and a negative stain ______________________________.
Respuesta
  • Stains what you want to see Stains everything but the structure you want to see
  • Stains everything but the structure you want to see Stains what you want to see
  • Stains gram (+) bacteria Stains gram (-) bacteria
  • Stains gram (-) bacteria Stains gram (+) bacteria

Pregunta 58

Pregunta
Why are Nigrosin and India ink commonly used negative stains?
Respuesta
  • They are negatively charged dyes and are repelled by the negative contents of the bacterial cell.
  • They are positively charged dyes and are repelled by the positive contents of the bacterial cell.
  • They are positively charged dyes and are attracted by the negative contents of the bacterial cell.
  • They are negatively charged dyes and are attracted by the positive contents of the bacterial cell.

Pregunta 59

Pregunta
What are the advantages of using a negative stain versus a positive stain?
Respuesta
  • Able to see a capsule
  • Do not heat fix so can see them as they are
  • Can be used for things that don't stain.
  • Can see if it produces endospores
  • Can see if it is gram (-) or gram (+)

Pregunta 60

Pregunta
Sticky substance that is tightly bound, highly organized and generally round or oval.
Respuesta
  • Capsule
  • Slime layer
  • Endospore

Pregunta 61

Pregunta
Sticky substance is loosely bound and somewhat irregularly shaped.
Respuesta
  • Capsule
  • Slime layer
  • Endospore

Pregunta 62

Pregunta
How does a capsule contribute to the virulence of a bacteria?
Respuesta
  • Helps the bacteria avoid phagocytosis by white blood cells
  • Helps the bacteria attach to skin and mucous membranes
  • Is a source of nutrition for the bacteria when resources are scarce
  • Contains the pathogenic part of the bacteria

Pregunta 63

Pregunta
What are the difference(s) between the smear preparation for a Gram stain and a capsule stain?
Respuesta
  • A smear for a capsule stain is not heat fixed.
  • A spear from a capsule stain is not rinsed with deionized water it is rinsed with copper sulfate
  • A smear for a capsule stain uses steam
  • A smear for a Gram stain uses crystal violet and a capsule stain does not

Pregunta 64

Pregunta
Using Anthony's stain, the background will be colored ____________________ and the capsule will be colored _________________________.
Respuesta
  • Dark blue Light blue to white
  • Light blue to white Dark blue

Pregunta 65

Pregunta
What are the damaging by-products of oxygen during cellular respiration?
Respuesta
  • Superoxides
  • Peroxides
  • Carbon dioxide
  • Carbon monoxide

Pregunta 66

Pregunta
What enzymes neutralize the damaging effects of the by-products of oxygen during cellular respiration?
Respuesta
  • Catalase
  • Peroxidase
  • Superoxide dismutase
  • Carbonase

Pregunta 67

Pregunta
Uses oxygen as the final member of the ETC and has enzymes to break down the toxic by-products of oxygen.
Respuesta
  • Aerobe
  • Anerobe

Pregunta 68

Pregunta
Does not use oxygen as the final member of the ETC and lacks the enzymes to breakdown the toxic by-products of oxygen.
Respuesta
  • Aerobe
  • Anaerobe

Pregunta 69

Pregunta
How does a candle jar achieve an atmosphere to grow microaerophilic and capnophilic organisms?
Respuesta
  • The flame burns using up the oxygen and producing CO2
  • The flame burns up the CO2 and produces oxygen.

Pregunta 70

Pregunta
How does a Gas Pak jar achieve an anaerobic atmosphere?
Respuesta
  • The pak contains chemicals that produce H+ and CO2. The H+ combines with the O2 in the jar to produce H2O.
  • The pak contains chemicals that produce CO2 and destroy O2.

Pregunta 71

Pregunta
Which type of organism grows equally well in all types of oxygen concentrations?
Respuesta
  • Facultative anaerobes
  • Microaerophiles
  • Capnophiles
  • Aerotolerant anaerobes

Pregunta 72

Pregunta
How does thioglycolate broth grow anaerobic bacteria as well as aerobic bacteria?
Respuesta
  • The agar prevents diffusion of O2
  • It contains a reducing agent, sodium thioglycollate, that combines with O2 to produce water
  • It contains a reducing agent, sodium thioglycollate, that combines with the agar to produce CO2 near the bottom
  • The agar prevents diffusion of CO2

Pregunta 73

Pregunta
Organisms that require around 20% atmospheric oxygen. (Check the name of the organism and the media on which it will grow.)
Respuesta
  • Strict aerobe
  • Facultative anaerobe
  • Only at the top of the thioglycollate broth.
  • Candle jar
  • Gas pak
  • Capnophile

Pregunta 74

Pregunta
Organisms that require between 5-15% atmospheric oxygen for growth (Check the name of the organism and the media on which it will grow.)
Respuesta
  • Microaerophile
  • Facultative anaerobe
  • Grows in candle jar
  • Grows at the point where the oxygen level is diminishing in the thio broth.
  • Grows in the GasPak

Pregunta 75

Pregunta
Organisms that require between 5-15% atmospheric oxygen and increased (10%) carbon dioxide. (Check the name of the organism and the media on which it will grow.)
Respuesta
  • Capnophile
  • Microaerophile
  • Grows in candle jar
  • Grows in oxygen
  • Grows in a GasPak

Pregunta 76

Pregunta
Organisms that will use a fermentative metabolic pathway in anaerobic conditions yet can convert to aerobic cellular respiration when atmospheric oxygen is present. They grow equally well in aerobic anaerobic environments.
Respuesta
  • Facultative anaerobe
  • Microaerophile
  • Grows in oxygen
  • Grows in candle jar
  • Grows in GasPak
  • Grows throughout the entire thio broth slant

Pregunta 77

Pregunta
Organism that can exist in the presence of oxygen, but does not use oxygen for metabolism.
Respuesta
  • Aerotolerant anaerobe
  • Microaerophile
  • All over the thio broth slant but slightly less at the top
  • Grows in the GasPak
  • Capnophile

Pregunta 78

Pregunta
Organisms that die in the presence of atmospheric oxygen because they lack the enzymes needed to breakdown the toxic by-products.
Respuesta
  • Strict anaerobe
  • Grows in GasPak
  • Grows in candle jar
  • Grows toward the bottom of the thio broth
  • Grows toward the top of the thio broth

Pregunta 79

Pregunta
Which gene allows jellyfish to glow in the dark?
Respuesta
  • Green fluorescent protein
  • Fluorescent blue plasmid
  • GFP
  • FBP

Pregunta 80

Pregunta
Bacteria cells able to take up the plasmid/DNA.
Respuesta
  • Competent cells
  • Competent genes
  • Competent proteins

Pregunta 81

Pregunta
What laboratory procedures are done to encourage cells to become competent?
Respuesta
  • Mixing cells with calcium chloride during growth phase
  • Exposing cells to extreme temperature changes during the proceduree
  • Mixing cells with with heated calcium hydroxide
  • Freezing cells

Pregunta 82

Pregunta
What is the relationship between a gene and a protein?
Respuesta
  • A gene is a piece of DNA that provides the instructions for making a protein
  • A protein is a piece of DNA that provides the instructions for making a gene
  • DNA is a piece of a protein that provides the instructions for making a gene

Pregunta 83

Pregunta
Chromosomal DNA ___________________________________ and plasmids _________________________________________.
Respuesta
  • Carries genes needed for the hereditary characteristics essential for bacterial growth and reproduction. Small pieces of circular DNA that have genes for one or more traits that may be beneficial but are not essential for bacterial survival.
  • Small pieces of circular DNA that have genes for one or more traits that may be beneficial but are not essential for bacterial survival. Carries genes needed for the hereditary characteristics essential for bacterial growth and reproduction.
  • Carries genes needed for the hereditary characteristics that may be beneficial but are not essential for bacterial growth and reproduction. Small pieces of circular DNA that have genes for one or more traits that are essential for bacterial survival.

Pregunta 84

Pregunta
Which genes are contained in the pGLO plasmid?
Respuesta
  • GFP
  • BLA
  • Ara-C
  • Ara-B

Pregunta 85

Pregunta
How is expression of the Green Fluorescent Protein gene regulated?
Respuesta
  • The Ara-C gene controls expression of GFP via the sugar arabinose
  • The Ara-B gene controls expression of GFP via the sugar arabinose
  • The Ara-C gene controls expression of GFP via the sugar glucose

Pregunta 86

Pregunta
Chemicals produced by microorganisms that in small quantities can inhibit the growth of other microorganisms
Respuesta
  • Antibiotic
  • MIC
  • E-test

Pregunta 87

Pregunta
Determines the lowest concentration of an antibiotic that is able to inhibit the growth of a test organism.
Respuesta
  • Antibiotic
  • MIC
  • E-test

Pregunta 88

Pregunta
A plastic coated strip that contains a gradient
Respuesta
  • Antibiotic
  • MIC
  • E-test

Pregunta 89

Pregunta
What are the disadvantages of using a broad spectrum antibiotic?
Respuesta
  • Contributes to escalating drug resistance
  • Wipes out a person's normal flora resulting in superinfections
  • Only works on weak bacteria

Pregunta 90

Pregunta
What does the antibiotic test result "sensitive" indicate about the test organism?
Respuesta
  • An organism is susceptible to drug concentrations obtained in the body using normal therapeutic doses.
  • The antibiotic is likely to be effective against the pathogen
  • The antibiotic is not likely to work.

Pregunta 91

Pregunta
What factors must be controlled in order to standardize the Kirby-Bauer test?
Respuesta
  • Stability of the antibiotic
  • Rate of diffusion of the antibiotic
  • pH of the culture medium
  • Depth of the culture medium
  • Inoculum density
  • Incubation time
  • Incubation temperature
  • Concentration of antibiotic

Pregunta 92

Pregunta
A?
Image:
Scope_1_A (image/jpg)
Respuesta
  • Arm
  • Rheostat
  • Power switch
  • Coarse focus knob

Pregunta 93

Pregunta
B?
Image:
Scope_1_A (image/jpg)
Respuesta
  • Rheostat
  • Power switch
  • Coarse focus knob
  • Arm

Pregunta 94

Pregunta
C?
Image:
Scope_1_A (image/jpg)
Respuesta
  • Rheostat
  • Power switch
  • Coarse focus knob
  • Fine focus knob

Pregunta 95

Pregunta
D?
Image:
Scope_1_A (image/jpg)
Respuesta
  • Coarse focus knob
  • Fine focus knob
  • RHeostat
  • Power switch

Pregunta 96

Pregunta
E?
Image:
Scope_1_A (image/jpg)
Respuesta
  • Fine focus knob
  • Coarse focus knob
  • Rheostat
  • Power switch

Pregunta 97

Pregunta
A?
Image:
Scope_2_A (image/jpg)
Respuesta
  • Ocular
  • Diopter
  • Diaphragm ring
  • Fine focus knob

Pregunta 98

Pregunta
B?
Image:
Scope_2_A (image/jpg)
Respuesta
  • Mechanical stage
  • Base
  • Slide stage
  • Ocular stage

Pregunta 99

Pregunta
C?
Image:
Scope_2_A (image/jpg)
Respuesta
  • Fine focus knob
  • Coarse focus knob

Pregunta 100

Pregunta
D?
Image:
Scope_2_A (image/jpg)
Respuesta
  • Base
  • Mechanical stage
  • Mechanical base

Pregunta 101

Pregunta
A B C D E
Image:
Scope_3_A (image/jpg)
Respuesta
  • A - Objective lens B - Iris diaphragm lever C - Condenser centering screw D - Base diaphragm E - Mechanical stage knob
  • A - Ocular lens B - Iris diaphragm lever C - Condenser centering screw D - Base diaphragm E - Mechanical stage knob
  • A - Ocular lens B - Condenser centering screw C - Iris diaphragm lever D - Base diaphragm E - Mechanical stage knob
  • A - Ocular lens B - Iris diaphragm lever C - Mechanical stage knob D - Base diaphragm E - Condenser centering screw

Pregunta 102

Pregunta
A B C
Image:
Scope_4_A (image/jpg)
Respuesta
  • A - Iris diaphragm lever B - Condenser centering screw C - Base diaphragm
  • A - Condenser centering screw B - Iris diaphragm lever C - Base diaphragm
  • A - Iris diopter screw B - Iris diaphragm lever C - Base diaphragm
  • A - Iris diopter lever B - Condenser centering screw C - Base diaphragm

Pregunta 103

Pregunta
A B
Image:
Scope_5_A (image/jpg)
Respuesta
  • A - Revolving nose piece B - Mechanical stage clip
  • A - Objective ring B - Mechanical stage clip
  • A - Diopter ring B - Mechanical stage clip

Pregunta 104

Pregunta
A B C
Respuesta
  • A - Condenser knob B - Condenser C - Base diaphragm ring
  • A - Condenser knob B - Condenser ring C - Base
  • A - Condenser ring B - Condenser C - Base

Pregunta 105

Pregunta
In the thioglycollate test tube A, what kind of organism does the growth represent?
Image:
thioglycollate_A (image/jpg)
Respuesta
  • Microaerophile
  • Obligate/Strict aerobe
  • Facultative anaerobe
  • Obigate/strict anaerbe

Pregunta 106

Pregunta
In the thioglycollate test tube B, what kind of organism does the growth represent?
Image:
thioglycollate_A (image/jpg)
Respuesta
  • Microaerophile
  • Obligate/Strict aerobe
  • Facultative anaerobe
  • Obligate anaerobe

Pregunta 107

Pregunta
In the thioglycollate test tube C, what kind of organism does the growth represent?
Image:
thioglycollate_A (image/jpg)
Respuesta
  • Microaerophile
  • Obligate/strict aerobe
  • Facultative anaerobe
  • Obligate/strict anaerobe

Pregunta 108

Pregunta
In the thioglycollate test tube D, what kind of organism does the growth represent?
Image:
thioglycollate_A (image/jpg)
Respuesta
  • Microaerophile
  • Obligate/strict aerobe
  • Facultative anaerobe
  • Obligate/strict anaerobe

Pregunta 109

Pregunta
On a luria plate with Ampicillin, why is there no E. coli growth?
Respuesta
  • Because E.coli are sensitive to Ampicillin
  • Because E. coli are resistant to Ampicillin
  • Because luria does not contain the nutrients necessary for growth.

Pregunta 110

Pregunta
What protein allowed E. coli to grow on the plate with luria, Ampicillin and pGlo?
Respuesta
  • Beta lactamase
  • BLA
  • GFP
  • Green fluorescent protein

Pregunta 111

Pregunta
What gene allowed the E. coli to grow on the plate with luria, Ampicillin and pGlo?
Respuesta
  • BLA
  • Beta lactamase
  • GFP
  • Green fluorescent protein

Pregunta 112

Pregunta
What molecule allowed the E. coli to glow like a jellyfish?
Respuesta
  • Arabinose
  • Beta lactamase
  • Ara-C
  • Green fluorescent protein

Pregunta 113

Pregunta
What media is used for the antibiotic sensitivity test?
Respuesta
  • Mueller hinton
  • Kirby Bauer
  • Luria

Pregunta 114

Pregunta
What is the result of the beta lactamase test pictured?
Image:
Beta_lactamase_test_negative_A (image/jpg)
Respuesta
  • It is pink and therefore, the test is negative and the microbe is sensitive to penicillin
  • it is pink and therefore, the test is positive and the microbe is sensitive to penicillin
  • it is pink and therefore, the test is positive and the microbe is resistant to penicillin
  • It is pink and therefore, the test is negative and the microbe is resistant to penicillin

Pregunta 115

Pregunta
Beta lactamase test
Image:
Beta_lactamase_test_positive_A (image/jpg)
Respuesta
  • It is yellow and therefore, the test is positive and the microbe is resistant to penicillin
  • It is yellow and therefore, the test is negative and the microbe is resistant to penicillin
  • It is yellow and therefore, the test is positive and the microbe is sensitive to penicillin
  • It is yellow and therefore, the test is negative and the microbe is sensitive to penicillin

Pregunta 116

Pregunta
Why us thioglycollate a semi-solid agar?
Respuesta
  • Has agar in it and contains a reducing agent that produces water
  • Has agar and a reducing agent that produces CO2

Pregunta 117

Pregunta
Why are agar plates labeled on the bottom and inverted when incubated?
Respuesta
  • Labeled on the bottom in case you lose the lid and inverted during incubation to prevent condensation from dripping onto the agar surface
  • Labeled on the bottom in case you lose the lid and inverted so you can read the label

Pregunta 118

Pregunta
Why hold the test tube with your little finger while taking your bacterial sample?
Respuesta
  • So you do not contaminate the cap.
  • So the cap doesn't roll away.
  • So you can stretch your fingers.

Pregunta 119

Pregunta
Describe the proteins encoded in the pGLO plasmid.
Respuesta
  • Green fluorescent protein
  • Beta lactamase
  • Ara-C represser protein
  • Beta green protein

Pregunta 120

Pregunta
What is the principal of the beta lactamase test?
Respuesta
  • Does the bacteria have the BLA gene and therefore produce beta lactamase
  • Does the bacteria have the BLA protein and therefore produce the beta lactamase gene

Pregunta 121

Pregunta
Explain the regulation mechanism of the Green Fluorescent Protein gene expression.
Respuesta
  • Arabinose has to move the Ara-C represser protein out of the way so GFP can be transcribed and translated.
  • GFP takes up the arabinose so Ara-C can be transcribed and translated.

Pregunta 122

Pregunta
Define reducing agent
Respuesta
  • Sodium thioglycollate is a reducing agent and it reduces by binding O2 and converting it to water
  • Sodium thioglycollate is a reducing agent that reduces by causing evaporation of the broth

Pregunta 123

Pregunta
What are the criteria used to describe a bacterial colony>
Respuesta
  • Surface
  • Elevation
  • Whole colony
  • Edge
  • Color

Pregunta 124

Pregunta
Locations of items in the lab
Respuesta
  • Near the front door: Fire extinguisher, Eye wash, Shower, Gloves
  • Back of room: Fume hood
  • Lab bench: Disinfectant, Autoclave bags, Paper towels
  • Near Sinks: Bleach, Dyes used for stains, Paper towels (above)
  • Fume hood: Broken glass disposal box (underneath), Autoclave bags
  • Front podium: Phone
  • Back: Test tube racks, MSDS notebooks (back corner),
  • Next to fridge: Dustpan and broom

Pregunta 125

Pregunta
Which items are disposed of in the autoclave trash?
Respuesta
  • Used glass test tubes
  • Used glass slides
  • Broken glass
  • Used latex gloves
  • Paper towels used for bench disinfection
  • Contaminated Petri plates

Pregunta 126

Pregunta
Which items should be disposed of in the disinfectant basin?
Respuesta
  • Used glass slides
  • Used glass test tubes
  • Contaminated Petri plates
  • Broken glass

Pregunta 127

Pregunta
Which itens should be disposed of in the regular trash?
Respuesta
  • Broken glass
  • Paper towels used in bench disinfection
  • Qtip swabs used to clean microscopes
  • Used latex gloves
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