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Pregunta 1
Pregunta
What is a genome?
Respuesta
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A little creature possessing telepathic powers
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An organism's entire genetic material
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The entire genetic material for one gene
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The nucleotide sequence of the introns
Pregunta 2
Pregunta
Why is it useful to determine the proteomes of simple organisms?
Respuesta
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To create better vaccines
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To allow monitoring of pathogens in outbreaks of disease
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So that we can alter our own genetics to make these proteins
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To learn more about introns
Pregunta 3
Pregunta
Why is it hard to translate the genome of complex organisms into their proteomes?
Respuesta
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They have large sections of non-coding DNA
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Their mRNA is hard to extract
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They contain complex regulatory genes
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Some genes are switched off so not all the possible proteins are made
Pregunta 4
Pregunta
What are the benefits of the new sequencing methods compared to the original method devised by Frederick Sanger?
Respuesta
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They are automated
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They are done on a much smaller scale so they are faster
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They cost less to do
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They involve less chemicals
Pregunta 5
Pregunta
Because DNA is [blank_start]universal[blank_end], the recipient of recombinant DNA does not have to be of the same species as the donor.
Respuesta
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universal
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complementary
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degenerate
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antiparallel
Pregunta 6
Pregunta
Making DNA fragments from mRNA
1. Extract target mRNA molecules from a cell that produces the required protein.
2. Mix the mRNA with free DNA nucleotides and [blank_start]reverse transcriptase[blank_end].
3. The mRNA is used as a template for new strands of [blank_start]cDNA[blank_end] to be synthesised that are complementary to it.
Respuesta
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reverse transcriptase
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a restriction endonuclease
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DNA ligase
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DNA polymerase
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cDNA
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tRNA
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rRNA
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miRNA
Pregunta 7
Pregunta
[blank_start]Restriction Endonucleases[blank_end] digest the DNA at specific [blank_start]recognition[blank_end] sequences. These sites are special because they are [blank_start]palindromic[blank_end], meaning that they consist of [blank_start]antiparallel[blank_end] base pairs.
Some types of this enzyme cut the DNA so that small tails of unpaired bases are created at each end of the fragment. These are called [blank_start]sticky[blank_end] ends and can be used to [blank_start]anneal[blank_end] DNA fragments together as long as the ends are complementary.
Respuesta
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Restriction Endonucleases
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Reverse Transcriptases
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recognition
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palindromic
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antiparallel
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sticky
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blunt
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anneal
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recconect
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digest
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