4640165
| Pregunta | Respuesta |
| Is Pseudomonas Gram Positive or Gram Negative? | Pseudomonas is GRAM NEGATIVE! |
| Name six features of P.aeruginosa | 1. gram- negative 2. motile 3. catalase- POSITIVE 4. Genetically diverse 5. Metabolically diverse 6. Resistant to many antibiotics |
| Where is this bacteria found? | Ubiquitous in soil and water |
| Who is at RISK for infection? | - RARELY in Healthy people (unless it gets past our innate IMR) -immunocompromised (i.e. AIDS, chemo) -CF patients, burn patients -cause of ventilator associated pneumonia (VAP) |
| What are some virulence factors in P. areuginosa? (Surface) | Pilli-> adherence to surfaces, twitching, movement Flagellum-> Movement |
| What are some virulence factors in P. aeruginosa? (Secreted) | phospholipase-> hydrolyses (Breaks down) host phospholipids elastase-> proteolyses (Breaks down) host proteins alginate-> stops host phagocytosis and helps biofilm formation |
| How do these bacteria encounter hosts? | -The bacteria is in many env'ts, ex. in poorly chlorinated swimming pools/hot tubs |
| How do these bacteria enter hosts? | ADHERE to host cell w/ Type 4 pilli (adhesisns) and flagella |
| How does P. aeruginosa move/ spread? | 3 Main Modes of Motility: -swimming (flagella) -twitching (type 4 pili) -swarming (flagella+ type 4 pili) |
| What state does swimming occur? | LIQUID |
| What state does Twitching occur? | SOLID |
| What State does Swarming Occur? | VISCOUS, Part Liquid/part solid |
| Name 3 factors that contribute to multiplication/ dissemination | 1. Resistance to neutrophil phagocytosis 2. Ability to damage tissue (ex. proteases, T3SS effectors) 3. Quorum sensing |
| What is Quorum sensing? | |
| How can P. be diagnosed? | -It cultured on Pseudomonas isolation agar -the colonies are green and smell like almonds and grapes |
| What Modes of Parthenogenesis Does this Bacteria have? |
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