The presence of 2 sugars on a culture
growth, the preferred sugar consumed
first, which leads to rapid growth,
followed by lag phase, and now the
cellular machinery used to metabolize
second sugar.
Special requirements
Vitamins
Thiamine, biotin
Organic nitrogen
do not fix atmosphere nitrogen
Amino acid transamination
Ammonia/Ammonium
not a good component because its use lowers pH, thereby inhibiting growth
Nitrate
Insufficiencies
Phosphorus
Increased activity of uptake systems
Iron
Essential donor and acceptor of electrons
Often captured by the use of hydroxamate siderophores
as heme
Sulphur
as in cysteine
Nutrient capture
Electrical field involved in nutrient uptake
Exterior apex is more
electronegative than the
exterior subapical
regions
Proton export through ATPase (ion
pump) and uptake of proton
through sitmultaneously transport
amino acid
Symport proteins are
located near the
apical tips, whereas
the ion pumps are in
the subapical regions
Yeast do not utilize insoluble erosion
zone because they may trapped in
their own substrate erosion zone
Enzymes are secreted at the
tips to degrade the polymers
and soluble nutrients are
absorbed supapically.
Antibiotics are subapically released
into the substrate erosion zone to
prevent other organisms from using
the enzyme digesting product.