1781204
Questão 1
Questão
Which property is not essential for the origin of life?
Responda
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The need to store information
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The need to duplicate information
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The need to change information
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The need to make proteins
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The need to convert into favorable reactions
Questão 2
Questão
Why is it thought that ribozymes are likely to be a legacy from an ancestral RNA world?
Responda
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They are more stable than RNAs
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When they act, they require the presence of protein cofactors
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They act without the participation of protein cofactors.
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They can be made spontaneously without a DNA template.
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They are made by proteins
Questão 3
Questão
What was probably the first carrier of genetic information in primitive organisms?
Responda
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DNA that could self-replicate
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Proteins
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Polypeptides
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RNA that could self-replicate
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Lipids
Questão 4
Questão
________ can be used to purify transcription activator proteins
Responda
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Gel mobility shift assays
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DNA affinity chromatography
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Centrifugation
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DNA footprinting
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ChIP assays
Questão 5
Questão
Sequence specific DNA binding proteins frequently have a region that binds in the major groove because:
Responda
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It is smaller
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It contains sequence specific information
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The minor groove is not available
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It is positively charged
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It is negatively charged
Questão 6
Questão
The following secondary structures are known to specifically interact with DNA (select all that apply):
Responda
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Alpha helix
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Beta sheet
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Unstructured loops
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Double helix
Questão 7
Questão
What happens at the lac operon in the absence of glucose and presence of lactose?
Responda
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No expression
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Low expression
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The lac repressor binds the promoter
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High expression
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The CAP protein doesn't bind to the promoter
Questão 8
Questão
Multiple binding sites for a repressor is advantageous because:
Responda
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There are too many repressors in cells
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DNA must be bent to allow transcription
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Repressors are only active when bound to many sites at once
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This increases the effective concentration of the repressor
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Repressors need multiple binding sites to bind ligands
Questão 9
Questão
An operator is:
Responda
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A protein
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A protein binding site
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The same as a promoter
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A type of repressor
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The start site for RNA synthesis
Questão 10
Questão
Mammalian x-chromosome inactivation:
Responda
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Occurs only in humans
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Prevents females from being mosaics
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Is not necessary
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Is necessary for dosage compensation
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Only occurs in males
Questão 11
Questão
CpG islands are generally found in
Responda
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Inactive genes
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Introns
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Exons
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Promoters of constantly active genes
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Promoters of rarely expressed genes
Questão 12
Questão
Deamination of methyl-cytosine:
Responda
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Does not occur
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Changes methyl-cytosine to thymine
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Only occurs in the sequence CG
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Results in genomic imprinting
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Occurs only in expressed genes
Questão 13
Questão
Sex determination in drosophila is due to:
Responda
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Riboswitches
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Alternative splicing
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Alternative 3' end cleavage
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Localization of mRNA
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Alternative translational initiation
Questão 14
Questão
Mirco RNAs (miRNAs):
Responda
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Are the same as rRNAs
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Are not encoded by the genome
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Are found only in bacteria
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Are involved in gene repression
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Are part of ribosomes
Questão 15
Questão
Attenuation involves regulation of:
Responda
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Translation
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Replication
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Recombination
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Transcription
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Proteolysis
Questão 16
Questão
Ion exchange chromatography separates:
Responda
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Proteins, based on size
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Proteins, based on surface charge
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Proteins, based on source
Questão 17
Questão
FRET is used to study:
Responda
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Protein structure
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Protein molecular weight
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Protein/protein interaction
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Protein sequence
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Protein denaturation
Questão 18
Questão
SDS is used in protein gels to (select all that apply)...
Responda
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Make glassware clean up easier
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Help denature proteins
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To create a uniform mass/charge ratio
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To reduce disulfide bonds
Questão 19
Questão
DNA libraries are used to bank (select all that apply):
Responda
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Genomic DNA
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RNA
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cDNA
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Artificial chromosomes
Questão 20
Questão
Dideoxy nucleotides are used in DNA sequences to:
Responda
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Denature the DNA
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Hybridize the DNA
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Chain terminate the DNA
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Elongate the DNA
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Transcribe the DNA
Questão 21
Questão
Hybridization of nucleic acids refers to:
Responda
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A renaturation technique
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An electrophoretic technique
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A cloning technique
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A forensic technique
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A purification technique
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