456291
Descrição
Quiz por gina_evans0312, atualizado more than 1 year ago
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Criado por gina_evans0312
quase 11 anos atrás
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Questão 1
Questão
Gene synthesis can be fully automated cheaply
Responda
- True
- False
Questão 2
Questão
Why is genome synthesis difficult?
Responda
-
Chemical synthesis is limited to 100nt at a time
-
Manipulation of large fragments is difficult
-
Vectors are limited
Questão 3
Questão
Synthesis of a genome must be done in small, overlapping parts
Responda
- True
- False
Questão 4
Questão
How many kbpairs can PCR be used for?
Responda
-
1-5
-
5-10
-
10-15
Questão 5
Questão
What is essential when creating small fragments to be joined together later in clones?
Responda
-
The fragments must overlap
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The fragments MUST be 7kb long
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The fragments cannot contain introns
Questão 6
Questão
When transplacting the synthetic genome between clones, what must not be present?
Responda
-
Proteins
-
DNA
-
RNA
Questão 7
Questão
How is the receptor organism for the synthetic genome made able to receive it?
Responda
-
The cell wall is treated with chemicals to make it permeable
-
The DNA is directly microinjected into the cell
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The cell absorbs the DNA with other nutrients
Questão 8
Questão
Once the synthetic genome has been added to the cell, it divides as it contains two genomes
Responda
- True
- False
Questão 9
Questão
Why synthetic organisms?
Responda
-
To see if we can
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To improve/redesign regulatory networks
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To produce protective genes
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Biofuels/biopharmecuticals
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