RAS Activation

Descripción

Year 2 Fichas sobre RAS Activation, creado por gina_evans0312 el 04/11/2013.
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Fichas por gina_evans0312, actualizado hace más de 1 año
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Creado por gina_evans0312 hace alrededor de 11 años
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Resumen del Recurso

Pregunta Respuesta
While inactive, tyrosine kinase exists as A monomer
The binding of the ligand causes the ....?... of tyrosine kinase Dimerisation
The structural change of tyrosine kinase causes Partial activation
What allows tyrosin kinase to become fully active? The phosphorylation of the activation lip
When the protein is fully activation, which protein is phosphorylated? Tyrosine kinase
This phosphorylation provides a docking site for which protein? GRB2
What does the activation of RAS require? A complex of protein linked to tyrosine kinase
Which part of GRB2 binds to GEF? SH3
The SH2 subunit of GRB2 binds where? To the phosphorylated tyrosine kinase
Why doesn't GRB2 bind to GEF all the time? Because only binding to phoshorylated T.K exposes the SH3 domain
Adaptor Protein Allows 2 unlike proteins to bind
What aa-rich domain does the SH3 domain of GRB2 bind to? The proline rich region of GEF
Where is inactive RAS found in a cell? In the membrane bound to GDP
What does GEF stand for? Guanine nucleotide exchange factor
Why does GEF binding activate RAS? It replaces GDP with GTP, activation it
What is the main function of active RAS? Downstream cascade causing cell division
How is RAS deactivated? GAP- GTPase activating protein causes GTP hydrolysis
How long is RAS active for (in general) One minute
How does GEF activate RAS? Inserts an alpha helix to open the switches, so GDP is replaced by GTP
Name the two aa that hold the RAS switch closed Gly60 & Thr 35
What is the first protein RAS activates? RAF
14-3-3 protein Binds to RAF and holds it in a folded state
Phosphorylation of what aa holds RAF inactive? Serine
The binding of RAS to RAF causes what to be displaced? 14-3-3 protein
RAS binding causes what to be phosphorylated? Kinase domain
RAS binding causes what to be dephosphorylated? The N terminus
Once RAF has been unfolded, it phosphorylates MEK
MAPK is phosphorylated by MEK
MEK is unusual because It can phosphorylate Ser, Thr & Tyr
Function of MPK Phosphorylation of nuclear transcription factors
How is MAPK activated? Phosphorylation of the activation lip
Name the P protein activated by MAPK p90-rsk
p90rsk activates PIPG
MEK stands for... MAP Kinase Erk Kinase
MEK stands for MAPK Erk Kianse
How does MEK phosphorylated MAPK? Tyrosine residue on activation lip, then the newly exposed Threonine residue
p90rsk is sensitive to... Insulin
PP1G stands for Protein phosphatase-1-G
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