1835758
Questão 1
Questão
What type of chromosome does G-banding "line-up"?
Responda
-
Metaphase
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Anaphase
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Telophase
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Prophase
Questão 2
Questão
What stain does G-banding use?
Responda
-
Giemsa
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Chrome red
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Methyl-blue
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Green
Questão 3
Questão
G-banding can only detect large abnormalities. True or false?
Responda
- True
- False
Questão 4
Questão
What does FISH stand for?
Responda
-
Fluorescent in situ hybridization
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Fluorescent in site hydrolysis
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Familial in situ hybridization
-
Familial in situ hydrolysis
Questão 5
Questão
You don't need to know what abnormality you are looking for when using FISH. True or false?
Responda
- True
- False
Questão 6
Questão
Why do you need to know what abnormality you are looking for when using FISH?
Responda
-
Because it uses fluorescent probes to bind to specific sequences
-
Because it used restriction enzymes marked with fluorescent probes to cut DNA at a specific sequence
Questão 7
Questão
How can you tell if there is an abnormality using the FISH technique?
Responda
-
Because some of the markers will not light up as they have not been able to anneal to bits of DNA sequence as they are missing
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Because all of the markers will light up because there are specific sequences of DNA that cause genetic disorders
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Because if there were no abnormalities the markers would not show up at all
Questão 8
Questão
FISH can show slightly smaller abnormalities than G-banding, but they still have to fairly large. True or false?
Responda
- True
- False
Questão 9
Questão
What does QF-PCR stand for?
Responda
-
Quantitative fluorescence Polymerase Chain Reaction
-
Qualitative fluorescence polymerase chain reaction
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Quantitative familial polymerase chain reaction
-
Qualitative familial polymerase chain reaction
Questão 10
Questão
What is QF-PCR used to detect?
Responda
-
Trisomy syndromes
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Abnormalities in chromosome size
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Deletion syndromes
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Abnormalities in chromosome banding
Questão 11
Questão
What is the name for trisomy 13?
Responda
-
Pattau's syndrome
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Edward's syndrome
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Down's syndrome
Questão 12
Questão
What is the name for trisomy 18?
Responda
-
Pattau's syndrome
-
Edward's syndrome
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Down's syndrome
Questão 13
Questão
What is the name for trisomy 21?
Responda
-
Pattau's syndrome
-
Edward's syndrome
-
Down's syndrome
Questão 14
Questão
Which biological technique is quickest?
Responda
-
FISH
-
G-banding
-
QF-PCR
Questão 15
Questão
You have to know what abnormality you are looking for when using QF-PCR. True or false?
Responda
- True
- False
Questão 16
Questão
What sort of abnormalities does array-CGH detect?
Responda
-
Larger abnormalities
-
Smaller abnormalities
Questão 17
Questão
What is DNA labelled with in array-CGH?
Responda
-
Fluorescent dye
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Coloured markers
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Radiation
Questão 18
Questão
What does the CGH in array-CGH stand for?
Responda
-
Comparative genomic hybridization
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Comparative genomic hydrolysis
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Comparative genetic hybridization
-
Comparative genetic hydrolysis
Questão 19
Questão
How can you tell if there is an abnormality in the DNA when using array-CGH?
Responda
-
The relative fluorescence of the sample and control strand will be different
-
The relative radiation of the sample and control strand will be different
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The length of the sample and control strand will be different when run through gel electrophoresis
Questão 20
Questão
Which two molecular biological techniques look at the actual base sequence when looking for genetic abnormalities?
Responda
-
G-banding
-
FISH
-
QF-PCR
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Array-CGH
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Sanger sequencing
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Next generation sequencing
Questão 21
Questão
Which of the following is cheaper and less time consuming?
Responda
-
Sanger sequencing
-
Next generation sequencing
Questão 22
Questão
Why do we only sequence exons when using sanger sequencing?
Responda
-
Because the introns do not code for proteins so are not important to us
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Because it is time consuming and expensive
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Because introns rarely have mutations that cause genetic diseases
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Because we are unable to isolate the introns to sequence them
Questão 23
Questão
What machine is used in sanger sequencing to read the sequence of bases?
Responda
-
An electropheragram
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A Geiger counter
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A microscope
Questão 24
Questão
Not all changes to the base sequence cause disease. True or false?
Responda
- True
- False
Questão 25
Questão
Why is it better to use next generation sequencing when you don't know what you are looking for?
Responda
-
Because it is easy to sequence a whole genome
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Because sanger sequencing would take too long
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Because next generation sequencing is more accurate
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Because next generation sequencing is less expensive
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